Strain identifier
BacDive ID: 160361
Type strain: 
Species: Lactococcus insecticola
Strain Designation: Hs20BO-1
Strain history: S. Noda; Univ. of Yamanashi, Japan; Hs20B0-1.
NCBI tax ID(s): 2709158 (species)
version 9.1 (current version)
General
@ref: 67267
BacDive-ID: 160361
DSM-Number: 110147
keywords: genome sequence, 16S sequence, Bacteria, Gram-positive
description: Lactococcus insecticola Hs20BO-1 is a Gram-positive bacterium that was isolated from Hodotermopsis sjostedti.
NCBI tax id
- NCBI tax id: 2709158
- Matching level: species
strain history
| @ref | history |
|---|---|
| 67267 | <- M. Sakamoto, RIKEN, Wako, Saitama, Japan; JCM 33485 <- S. Noda, University of Yamanashi, Kofu, Japan; JCM 33485 |
| 67770 | S. Noda; Univ. of Yamanashi, Japan; Hs20B0-1. |
doi: 10.13145/bacdive160361.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Streptococcaceae
- genus: Lactococcus
- species: Lactococcus insecticola
- full scientific name: Lactococcus insecticola Noda et al. 2020
@ref: 67267
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Streptococcaceae
genus: Lactococcus
species: Lactococcus insecticola
full scientific name: Lactococcus insecticola Noda et al. 2020
strain designation: Hs20BO-1
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | no | 95.5 | |
| 69480 | 92.27 | positive |
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 67267 | PYG MEDIUM (MODIFIED) (DSMZ Medium 104) | yes | https://mediadive.dsmz.de/medium/104 | Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water |
| 67267 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 67267 | positive | growth | 30 |
| 67770 | positive | growth | 30 |
Physiology and metabolism
spore formation
- @ref: 69481
- spore formation: no
- confidence: 100
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | host species |
|---|---|---|---|---|---|---|
| 67267 | Hodotermopsis sjostedti | Amami Oshima , Kagoshima Prefecture | Japan | JPN | Asia | |
| 67770 | Gut of the wood-feeding lower termite Hodotermopsis sjostedti | Hodotermopsis sjostedti |
Sequence information
16S sequences
- @ref: 67770
- description: Lactococcus insecticola Hs20B0_1 gene for 16S ribosomal RNA, partial sequence
- accession: LC523829
- length: 1483
- database: nuccore
- NCBI tax ID: 2709158
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Lactococcus sp. Hs20B0-1 | 2709158.3 | wgs | patric | 2709158 |
| 67770 | Lactococcus insecticola Hs20B0-1 | GCA_011170065 | contig | ncbi | 2709158 |
GC content
- @ref: 67770
- GC-content: 42.2
- method: genome sequence analysis
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69481 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 100 | no |
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 92.27 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 80.766 | no |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 84.461 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 98.373 | no |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 96.957 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 95.5 | no |
External links
@ref: 67267
culture collection no.: DSM 110147, JCM 33485
straininfo link
- @ref: 112610
- straininfo: 398969
literature
- topic: Phylogeny
- Pubmed-ID: 32614762
- title: Lactococcus insecticola sp. nov. and Lactococcus hodotermopsidis sp. nov., isolated from the gut of the wood-feeding lower termite Hodotermopsis sjostedti.
- authors: Noda S, Koyama F, Aihara C, Ikeyama N, Yuki M, Ohkuma M, Sakamoto M
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.004309
- year: 2020
- mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gastrointestinal Tract/*microbiology, Genes, Bacterial, Isoptera/*microbiology, Lactococcus/*classification/isolation & purification, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Wood
- topic2: Transcriptome
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 67267 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-110147 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 110147) | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 112610 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID398969.1 |