Strain identifier

BacDive ID: 159881

Type strain: Yes

Species: Pseudaeromonas paramecii

Strain Designation: PCS8

Strain history: <- Mann Kyoon SHIN, Univ. Of Ulsan

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General

@ref: 66555

BacDive-ID: 159881

keywords: 16S sequence, Bacteria, facultative anaerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Pseudaeromonas paramecii PCS8 is a facultative anaerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from monoclonal culture of ciliate protozoan P. caudatum from brackish riverwater.

strain history

@refhistory
67770M. K. Shin; Univ. of Ulsan, South Korea; PCS8.
67771<- Mann Kyoon SHIN, Univ. Of Ulsan

doi: 10.13145/bacdive159881.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Aeromonadales
  • family: Aeromonadaceae
  • genus: Pseudaeromonas
  • species: Pseudaeromonas paramecii
  • full scientific name: Pseudaeromonas paramecii Akter and Shin 2018

@ref: 66555

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Aeromonadales

family: Aeromonadaceae

genus: Pseudoaeromonas

species: Pseudoaeromonas paramecii

strain designation: PCS8

type strain: yes

Morphology

cell morphology

  • @ref: 66555
  • gram stain: negative
  • cell length: 1.5-2.8 µm
  • cell width: 0.5-1.0 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: monotrichous, polar

colony morphology

@refcolony colorcolony shapeincubation periodmedium used
66555shiny whitecircular1 dayNutrient agar (NA)
66555pinkMacConkey
66555dark-centered with green metallic sheeneosin methylene blue agar

Culture and growth conditions

culture medium

@refnamegrowth
66555eosin methylene blueyes
66555eosin methylene blue agaryes
66555MacConkeyyes
66555MHAyes
66555Nutrient agar (NA)yes
66555tryptic soy agar (TSA)yes

culture temp

@refgrowthtypetemperaturerange
66555positivegrowth12-44
66555positiveoptimum36-37mesophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
66555positiveoptimum7
66555positivegrowth6-10alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
66555facultative anaerobe
67771aerobe

spore formation

  • @ref: 66555
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentrationhalophily level
66555NaClpositivegrowth3 %
66555NaClpositiveoptimum1-2 %slightly halophilic

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6655517019(R)-amygdalin-builds acid from
6655528053melibiose-builds acid from
6655512936D-galactose+builds acid from
6655516024D-mannose+builds acid from
6655516899D-mannitol+builds acid from
6655517057cellobiose+builds acid from
6655517234glucose+builds acid from
6655517268myo-inositol+builds acid from
6655517306maltose+builds acid from
6655517716lactose+builds acid from
6655517754glycerol+builds acid from
6655517924D-sorbitol+builds acid from
6655517992sucrose+builds acid from
6655528757fructose+builds acid from
6655530849L-arabinose+builds acid from
6655562345L-rhamnose+/-builds acid from
6655517019(R)-amygdalin-carbon source
6655528053melibiose-carbon source
6655553258citric acid-carbon source
6655512936D-galactose+carbon source
6655516024D-mannose+carbon source
6655516899D-mannitol+carbon source
6655517057cellobiose+carbon source
6655517234glucose+carbon source
6655517268myo-inositol+carbon source
6655517306maltose+carbon source
6655517716lactose+carbon source
6655517754glycerol+carbon source
6655517924D-sorbitol+carbon source
6655517992sucrose+carbon source
6655528757fructose+carbon source
6655530849L-arabinose+carbon source
6655562345L-rhamnose+/-carbon source
6655517234glucose+fermentation
6655553424tween 20-hydrolysis
6655553426tween 80-hydrolysis
66555casein-hydrolysis
665554853esculin+hydrolysis
6655528017starch+hydrolysis
6655517632nitrate-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.
6655573908Vibriostatic agent O/129yesyes150 µg (disc)
6655528971ampicillinyesyes10 µg (disc)
6655517833gentamicinyesyes10 µg (disc)
665556104kanamycinyesyes30 µg (disc)
6655518208penicillin gyesyes10 Unit (disc)
6655517076streptomycinyesyes10 µg (disc)
6655550845doxycyclineyesyes30 µg (disc)
6655527902tetracyclineyesyes3 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
6655515688acetoinno
6655516136hydrogen sulfideno
6655535581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-test
6655517234glucose+
6655515688acetoin-

enzymes

@refvalueactivityec
66555arginine dihydrolase-3.5.3.6
66555catalase-1.11.1.6
66555cytochrome oxidase-1.9.3.1
66555gelatinase-
66555lysine decarboxylase-4.1.1.18
66555ornithine decarboxylase-4.1.1.17
66555tryptophan deaminase-4.1.99.1
66555urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66555C18:01
    66555C12:0 3OH0.1
    66555C16:0 3OH0.1
    66555C10:00.2
    66555C17:00.2
    66555C18:1 w9c0.3
    66555C20:1 w7c0.4
    66555C18:2 w6,9c / anteiso-C18:00.5
    66555C18:1 w7c / C18:1 w6c10.8
    66555C12:03.9
    66555C16:1 w7c / C16:1 w6c35.1
    66555C16:035.8
    66555C14:05.4
    66555C14:0 3OH / iso-C16:1 I / C12:0 aldehyde6.3
  • type of FA analysis: whole cell analysis
  • incubation medium: MHA
  • incubation temperature: 30
  • incubation time: 2
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 2001

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
66555monoclonal culture of ciliate protozoan P. caudatum from brackish riverwaterParamecium caudatumTaehwagang River, UlsanRepublic of KoreaKORAsia35.55129.288TSA2 days37ciliate cells were starved in steriliyed source water for 24 h and washed in source water containing penicillin (5000 U/ml), neomycin sulfate (50 µg/ml) and streptomycin (4000 µg/ml)
67770Monoclonal culture of the ciliate protozoan Paramecium caudatum from the Taehwagang River in UlsanParamecium caudatumRepublic of KoreaKORAsia
67771From Paramecium caudatum, Taehwa River(brackish water)UlsanRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Brackish
#Environmental#Aquatic#River (Creek)
#Host#Microbial

Sequence information

16S sequences

  • @ref: 66555
  • description: Pseudaeromonas paramecii strain PCS8 16S ribosomal RNA gene, partial sequence
  • accession: MF185818
  • length: 1394
  • database: ena
  • NCBI tax ID: 2138166

GC content

  • @ref: 66555
  • GC-content: 66.50
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 66555

culture collection no.: KCTC 62038, JCM 32226

literature

  • topic: Phylogeny
  • Pubmed-ID: 29664361
  • title: Pseudaeromonas paramecii sp. nov., isolated from the ciliate Paramecium caudatum and emendation of the genus Pseudaeromonas.
  • authors: Akter S, Shin MK
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002746
  • year: 2018

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66555Sumi Akter and Mann Kyoon ShinPseudaeromonas paramecii sp. nov., isolated from the ciliate Paramecium caudatum and emendation of the genus Pseudaeromonas10.1099/ijsem.0.002746IJSEM 68: 1845-1850 201829664361
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc