Strain identifier

BacDive ID: 159844

Type strain: Yes

Species: Marinobacter bohaiensis

Strain Designation: T17

Strain history: <- Dandan Wang, Ocean Univ. of China

NCBI tax ID(s): 2201898 (species)

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General

@ref: 66739

BacDive-ID: 159844

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Marinobacter bohaiensis T17 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from the benthic sediment of Jiaozhou Bay, Bohai Sea.

NCBI tax id

  • NCBI tax id: 2201898
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Dandan Wang, Ocean Univ. of China

doi: 10.13145/bacdive159844.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Alteromonadaceae
  • genus: Marinobacter
  • species: Marinobacter bohaiensis
  • full scientific name: Marinobacter bohaiensis Xu et al. 2018

@ref: 66739

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Alteromonadaceae

genus: Marinobacter

species: Marinobacter bohaiensis

strain designation: T17

type strain: yes

Morphology

cell morphology

  • @ref: 66739
  • gram stain: negative
  • cell length: 1.3-2.2 µm
  • cell width: 0.6-1.2 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: polar

colony morphology

@refcolony colorcolony shapeincubation periodmedium used
66739light yellowcircular4 daysMH medium
66739opaque and creamycircularMarine agar (MA)

Culture and growth conditions

culture medium

@refnamegrowthcomposition
66739Marine agar (MA)yes
66739MH mediumyesATCC medium: 1689 MH medium (containing per litre distilled water: 1 g glucose, 5 g proteose peptone, 5 g yeast extract) and supplemented with 7.5 % (w/v) total salts

culture temp

@refgrowthtypetemperaturerange
66739positiveoptimum35mesophilic
66739positivegrowth15-40
67771positivegrowth30-35mesophilic

culture pH

@refabilitytypepHPH range
66739positivegrowth5-10alkaliphile
66739positiveoptimum7.0-8.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
66739aerobe
67771aerobe

halophily

@refsaltgrowthtested relationconcentration
66739NaClpositivegrowth1.0-15.0 %(w/v)
66739NaClpositiveoptimum6.0-10.0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6673916024D-mannose-assimilation
6673916899D-mannitol-assimilation
6673917128adipate-assimilation
6673917306maltose-assimilation
6673917634D-glucose-assimilation
6673918401phenylacetate-assimilation
6673925115malate-assimilation
6673927689decanoate-assimilation
6673930849L-arabinose-assimilation
6673932032potassium gluconate-assimilation
6673953258sodium citrate-assimilation
6673959640N-acetylglucosamine-assimilation
667396731melezitose-builds acid from
6673912936D-galactose-builds acid from
6673915443inulin-builds acid from
6673915824D-fructose-builds acid from
6673915963ribitol-builds acid from
6673916024D-mannose-builds acid from
6673916443D-tagatose-builds acid from
6673916634raffinose-builds acid from
6673916813galactitol-builds acid from
6673916899D-mannitol-builds acid from
6673916988D-ribose-builds acid from
6673917057cellobiose-builds acid from
6673917108D-arabinose-builds acid from
6673917113erythritol-builds acid from
6673917151xylitol-builds acid from
6673917234glucose-builds acid from
6673917266L-sorbose-builds acid from
6673917268myo-inositol-builds acid from
6673917306maltose-builds acid from
6673917634D-glucose-builds acid from
6673917716lactose-builds acid from
6673917754glycerol-builds acid from
6673917814salicin-builds acid from
6673917924D-sorbitol-builds acid from
6673917992sucrose-builds acid from
6673918287L-fucose-builds acid from
6673918305arbutin-builds acid from
6673918333D-arabitol-builds acid from
6673918403L-arabitol-builds acid from
6673927082trehalose-builds acid from
6673927613amygdalin-builds acid from
6673928017starch-builds acid from
6673928053melibiose-builds acid from
6673928066gentiobiose-builds acid from
6673928087glycogen-builds acid from
6673928847D-fucose-builds acid from
6673930849L-arabinose-builds acid from
6673932032potassium gluconate-builds acid from
6673932528turanose-builds acid from
6673943943methyl alpha-D-mannoside-builds acid from
6673959640N-acetylglucosamine-builds acid from
6673962318D-lyxose-builds acid from
6673962345L-rhamnose-builds acid from
6673965327D-xylose-builds acid from
6673965328L-xylose-builds acid from
6673974863methyl beta-D-xylopyranoside-builds acid from
66739320061methyl alpha-D-glucopyranoside-builds acid from
66739esculin ferric citrate-builds acid from
66739potassium 2-dehydro-D-gluconate-builds acid from
66739potassium 5-dehydro-D-gluconate-builds acid from
6673916411acetic acid-carbon source
6673917268myo-inositol-carbon source
6673917306maltose-carbon source
6673917716lactose-carbon source
6673917924D-sorbitol-carbon source
6673927082trehalose-carbon source
6673916024D-mannose+carbon source
6673916236ethanol+carbon source
6673916899D-mannitol+carbon source
6673917234glucose+carbon source
6673917754glycerol+carbon source
6673923652dextrin+carbon source
6673924265gluconate+carbon source
6673924898isoleucine+carbon source
6673928260galactose+carbon source
6673928757fructose+carbon source
6673930849L-arabinose+carbon source
6673953258citric acid+carbon source
6673917895L-tyrosine-hydrolysis
667394853esculin-hydrolysis
667395291gelatin-hydrolysis
6673916991dna-hydrolysis
66739casein-hydrolysis
6673928017starch+hydrolysis
6673953424tween 20+hydrolysis
6673953425tween 60+hydrolysis
6673953426tween 80+hydrolysis
6673917632nitrate-reduction

metabolite production

@refChebi-IDmetaboliteproduction
6673916136hydrogen sulfideyes
6673935581indoleno

enzymes

@refvalueactivityec
66739alpha-chymotrypsin-3.4.21.1
66739alpha-fucosidase-3.2.1.51
66739alpha-galactosidase-3.2.1.22
66739alpha-mannosidase-3.2.1.24
66739beta-galactosidase-3.2.1.23
66739beta-glucosidase-3.2.1.21
66739beta-glucuronidase-3.2.1.31
66739gelatinase-
66739N-acetyl-beta-glucosaminidase-3.2.1.52
66739phenylalanine deaminase-4.3.1.5
66739trypsin-3.4.21.4
66739acid phosphatase+3.1.3.2
66739alkaline phosphatase+3.1.3.1
66739alpha-glucosidase+3.2.1.20
66739arginine dihydrolase+3.5.3.6
66739catalase+1.11.1.6
66739cystine arylamidase+3.4.11.3
66739cytochrome oxidase+1.9.3.1
66739esterase (C 4)+
66739esterase Lipase (C 8)+
66739leucine aminopeptidase+3.4.1.1
66739leucine arylamidase+3.4.11.1
66739lipase (C 14)+
66739naphthol-AS-BI-phosphohydrolase+
66739urease+3.5.1.5
66739valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66739cyclo-C19:0 w8c1.4
    66739C12:013.6
    66739C16:1 w7c / iso-C15:0 2OH15.2
    66739C16:0 10-OH16.8
    66739cyclo-C17:02.5
    66739C16:023.2
    66739C14:0 3OH / iso-C16:04.9
    66739C18:1 w7c6.5
    66739C12:0 3OH8.4
  • type of FA analysis: whole cell analysis
  • incubation medium: marine agar
  • agar/liquid: agar
  • incubation temperature: 30
  • software version: Sherlock 6.0
  • library/peak naming table: TSBA 6.0
  • system: MIS MIDI
  • instrument: Hewlett packard 6890
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationisolation procedure
66739the benthic sediment of Jiaozhou Bay, Bohai SeaJiaozhou Bay, Bohai SeaChinaCHNAsia36.0722120.308marine agar 22167 daysStrain T17 was isolated from the benthic sediment of Jiaozhou Bay, Bohai Sea, China. Approximately 1 g of the sample was placed in a test tube containing 5 ml sterile saline, and then was shaken in an oscillator for 5 min. After vortexing, the sample was diluted 10 times with sterile saline and spread on marine agar. Strain was isolated after 7 days of incubation.
67771From sediment sampled at Xiaogangon the east coast of Jiaozhou bayChinaCHNAsia36.0722120.308

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_104865.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_89;96_3539;97_8939;98_10959;99_104865&stattab=map
  • Last taxonomy: Marinobacter bohaiensis subclade
  • 16S sequence: KY628816
  • Sequence Identity:
  • Total samples: 9
  • soil counts: 4
  • aquatic counts: 5

Sequence information

16S sequences

  • @ref: 66739
  • description: Marinobacter sp. strain T17 16S ribosomal RNA gene, partial sequence
  • accession: KY628816
  • length: 1463
  • database: ena
  • NCBI tax ID: 50741

Genome sequences

  • @ref: 66792
  • description: Marinobacter bohaiensis T17
  • accession: GCA_003258515
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 2201898

GC content

  • @ref: 66739
  • GC-content: 63
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedyes89.929yes
gram-positiveno98.625no
anaerobicno98.716yes
aerobicyes95.674yes
halophileyes87.754yes
spore-formingno96.233no
glucose-utilyes82.804no
thermophileno99.563yes
motileyes91.365yes
glucose-fermentno86.191no

External links

@ref: 66739

culture collection no.: KCTC 52710, MCCC 1K03282

literature

  • topic: Phylogeny
  • Pubmed-ID: 30231958
  • title: Marinobacter bohaiensis sp. nov., a moderate halophile isolated from benthic sediment of the Bohai Sea.
  • authors: Xu S, Wang D, Wei Y, Cui Q, Li W
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003025
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Marinobacter/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66739Shanshan Xu, Dandan Wang, Yuxi Wei, Qiu Cui, Wenli LiMarinobacter bohaiensis sp. nov., a moderate halophile isolated from benthic sediment of the Bohai Sea10.1099/ijsem.0.003025IJSEM 68: 3534-3539 201830231958
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/