Strain identifier

BacDive ID: 159834

Type strain: Yes

Species: Motiliproteus coralliicola

Strain Designation: C34

Strain history: <- Guanghua Wang, Guangxi Univ.

NCBI tax ID(s): 2283196 (species)

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General

@ref: 66703

BacDive-ID: 159834

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, ovoid-shaped, colony-forming

description: Motiliproteus coralliicola C34 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from coral sample of a Porites species.

NCBI tax id

  • NCBI tax id: 2283196
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Guanghua Wang, Guangxi Univ.

doi: 10.13145/bacdive159834.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Oceanospirillaceae
  • genus: Motiliproteus
  • species: Motiliproteus coralliicola
  • full scientific name: Motiliproteus coralliicola Wang et al. 2018

@ref: 66703

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Oceanospirillaceae

genus: Motiliproteus

species: Motiliproteus coralliicola

strain designation: C34

type strain: yes

Morphology

cell morphology

  • @ref: 66703
  • gram stain: negative
  • cell length: 1.0-2.2 µm
  • cell width: 0.6-0.7 µm
  • cell shape: ovoid-shaped
  • motility: yes
  • flagellum arrangement: monotrichous, polar

colony morphology

  • @ref: 66703
  • colony color: light brown
  • colony shape: circular
  • medium used: 2216E agar

Culture and growth conditions

culture medium

  • @ref: 66703
  • name: 2216E agar
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
66703positiveoptimum30mesophilic
66703positivegrowth20-37
67771positivegrowth25mesophilic

culture pH

@refabilitytypepHPH range
66703positiveoptimum8
66703positivegrowth6-10alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
66703aerobe
67771aerobe

spore formation

  • @ref: 66703
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
66703NaClpositivegrowth1-8 %(w/v)
66703NaClpositiveoptimum4 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
667036731melezitose-builds acid from
6670312936D-galactose-builds acid from
6670315443inulin-builds acid from
6670315824D-fructose-builds acid from
6670315963ribitol-builds acid from
6670316024D-mannose-builds acid from
6670316443D-tagatose-builds acid from
6670316634raffinose-builds acid from
6670316813galactitol-builds acid from
6670316899D-mannitol-builds acid from
6670316988D-ribose-builds acid from
6670317057cellobiose-builds acid from
6670317108D-arabinose-builds acid from
6670317113erythritol-builds acid from
6670317151xylitol-builds acid from
6670317266L-sorbose-builds acid from
6670317268myo-inositol-builds acid from
6670317306maltose-builds acid from
6670317634D-glucose-builds acid from
6670317716lactose-builds acid from
6670317754glycerol-builds acid from
6670317814salicin-builds acid from
6670317924D-sorbitol-builds acid from
6670317992sucrose-builds acid from
6670318287L-fucose-builds acid from
6670318305arbutin-builds acid from
6670318333D-arabitol-builds acid from
6670318403L-arabitol-builds acid from
6670327082trehalose-builds acid from
6670327613amygdalin-builds acid from
6670328017starch-builds acid from
6670328053melibiose-builds acid from
6670328066gentiobiose-builds acid from
6670328087glycogen-builds acid from
6670328847D-fucose-builds acid from
6670330849L-arabinose-builds acid from
6670332032potassium gluconate-builds acid from
6670332528turanose-builds acid from
6670343943methyl alpha-D-mannoside-builds acid from
6670359640N-acetylglucosamine-builds acid from
6670362318D-lyxose-builds acid from
6670362345L-rhamnose-builds acid from
6670365327D-xylose-builds acid from
6670365328L-xylose-builds acid from
6670374863methyl beta-D-xylopyranoside-builds acid from
66703320061methyl alpha-D-glucopyranoside-builds acid from
66703esculin ferric citrate-builds acid from
66703potassium 2-dehydro-D-gluconate-builds acid from
66703potassium 5-dehydro-D-gluconate-builds acid from
6670328017starch-hydrolysis
6670353423tween 40-hydrolysis
6670353424tween 20-hydrolysis
6670353425tween 60-hydrolysis
6670353426tween 80-hydrolysis
6670313705acetoacetate+oxidation
6670317632nitrate+reduction
667036359lactulose-respiration
667038391D-gluconate-respiration
6670312936D-galactose-respiration
6670314314D-glucose 6-phosphate-respiration
6670314336glycerol 1-phosphate-respiration
6670315603L-leucine-respiration
6670315729L-ornithine-respiration
6670315740formate-respiration
6670315792malonate-respiration
6670315824D-fructose-respiration
6670315895D-galactonic acid lactone-respiration
6670315963ribitol-respiration
6670316000ethanolamine-respiration
6670316024D-mannose-respiration
6670316383cis-aconitate-respiration
6670316523D-serine-respiration
6670316634raffinose-respiration
6670316704uridine-respiration
66703167244-hydroxybutyrate-respiration
66703167632-oxobutanoate-respiration
6670316857L-threonine-respiration
6670316865gamma-aminobutyric acid-respiration
6670316899D-mannitol-respiration
6670316977L-alanine-respiration
6670317057cellobiose-respiration
6670317113erythritol-respiration
6670317115L-serine-respiration
6670317126DL-carnitine-respiration
6670317148putrescine-respiration
6670317151xylitol-respiration
6670317196L-asparagine-respiration
6670317203L-proline-respiration
6670317240itaconate-respiration
6670317268myo-inositol-respiration
6670317295L-phenylalanine-respiration
6670317306maltose-respiration
6670317596inosine-respiration
6670317716lactose-respiration
6670317748thymidine-respiration
6670317754glycerol-respiration
6670317784D-glucosaminic acid-respiration
6670317924D-sorbitol-respiration
6670317925alpha-D-glucose-respiration
6670317992sucrose-respiration
6670318024D-galacturonic acid-respiration
66703181014-hydroxyphenylacetic acid-respiration
6670318183L-pyroglutamic acid-respiration
66703182404-hydroxy-L-proline-respiration
6670318287L-fucose-respiration
6670318333D-arabitol-respiration
6670321217L-alaninamide-respiration
6670323652dextrin-respiration
6670324996lactate-respiration
6670326490quinate-respiration
6670327082trehalose-respiration
6670327248urocanic acid-respiration
6670327605D-psicose-respiration
6670328053melibiose-respiration
6670328066gentiobiose-respiration
6670328087glycogen-respiration
66703286442-oxopentanoate-respiration
6670328800N-acetylgalactosamine-respiration
6670329042glucose 1-phosphate-respiration
6670329985L-glutamate-respiration
6670329991L-aspartate-respiration
6670330031succinate-respiration
6670330612D-glucarate-respiration
6670330849L-arabinose-respiration
66703370543-hydroxybutyrate-respiration
6670340585alpha-cyclodextrin-respiration
6670341865sebacic acid-respiration
6670350048phenylethylamine-respiration
6670351850methyl pyruvate-respiration
6670353258citric acid-respiration
6670353423tween 40-respiration
6670353426tween 80-respiration
6670359640N-acetylglucosamine-respiration
6670362345L-rhamnose-respiration
66703645522-hydroxybutyrate-respiration
6670373706bromosuccinate-respiration
6670373784glycyl-L-glutamate-respiration
6670373786L-alanylglycine-respiration
6670373804glycyl L-aspartic acid-respiration
6670375146monomethyl succinate-respiration
66703143136succinamate-respiration
66703320055methyl beta-D-glucopyranoside-respiration
66703620642,3-butanediol-respiration
66703168102-oxoglutarate-respiration
6670315570D-alanine+respiration
6670315748D-glucuronate+respiration
6670315971L-histidine+respiration
6670316411acetic acid+respiration
6670317272propionate+respiration
6670332323glucuronamide+respiration
6670332528turanose+respiration

metabolite production

  • @ref: 66703
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
66703catalase-1.11.1.6
66703acid phosphatase+3.1.3.2
66703alkaline phosphatase+3.1.3.1
66703cytochrome oxidase+1.9.3.1
66703esterase (C 4)+
66703esterase Lipase (C 8)+
66703leucine arylamidase+3.4.11.1
66703naphthol-AS-BI-phosphohydrolase+
66703valine arylamidase+

Isolation, sampling and environmental information

isolation

@refsample typehost speciessampling dategeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
66703coral sample of a Porites speciesPoritesMarch 2017Weizhou Island, Beibu GulfChinaCHNAsia21.0617109.143one-tenth strength 2216E agarone-tenth weight of 2216E agar powder, three-fourths volume natural seawater, one-fourth volume distilled water and 13.5 g/l agar powder2 weeks25About 4 cm3 coral sample was washed with axenic natural seawater, and then soaked in axenic natural seawater at room temperature in a 50 ml centrifuge tube until bacterial isolation. The sample was homogenized with vortex, then diluted by 10-fold with axenic natural seawater and spread on one-tenth strength 2216E agar. Bacteria was incubated at 25°C for 2 weeks
67771From coralthe Beibu GulfChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Host#Invertebrates (Other)#Cnidaria (Corals)
#Environmental#Aquatic#Coral reef

Sequence information

16S sequences

  • @ref: 66703
  • description: Motiliproteus sp. strain C34 16S ribosomal RNA gene, partial sequence
  • accession: MH071780
  • length: 1502
  • database: ena
  • NCBI tax ID: 2283196

Genome sequences

  • @ref: 66792
  • description: Motiliproteus coralliicola C34
  • accession: GCA_003345655
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 2283196

GC content

  • @ref: 66703
  • GC-content: 56.7
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes91.65yes
flagellatedyes93.723yes
gram-positiveno99.079no
anaerobicno96.093yes
aerobicno58.18no
halophileyes66.224no
spore-formingno95.075yes
glucose-utilyes65.26yes
thermophileno99.417yes
glucose-fermentno83.375no

External links

@ref: 66703

culture collection no.: MCCC 1K03462, KCTC 62319

literature

  • topic: Phylogeny
  • Pubmed-ID: 30117801
  • title: Motiliproteus coralliicola sp. nov., a bacterium isolated from coral.
  • authors: Wang G, Xu S, Su H, Chen B, Huang W, Liang J, Wang Y, Yu K
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002984
  • year: 2018
  • mesh: Animals, Anthozoa/*microbiology, Bacteria/genetics, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Islands, Oceanospirillaceae/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66703Guanghua Wang, Shuailiang Xu, Hongfei Su,Biao Chen,Wen Huang, Jiayuan Liang, Yinghui Wang,Kefu YuMotiliproteus coralliicola sp. nov., a bacterium isolated from coral10.1099/ijsem.0.002984IJSEM 68: 3292-3295 201830117801
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc