Strain identifier

BacDive ID: 159805

Type strain: Yes

Species: Paenibacillus paeoniae

Strain Designation: M4BSY-1

NCBI tax ID(s): 2292705 (species)

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General

@ref: 66752

BacDive-ID: 159805

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped, colony-forming

description: Paenibacillus paeoniae M4BSY-1 is an aerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from a surfacesterilized leaf of Paeonia lactiflora Pall.

NCBI tax id

  • NCBI tax id: 2292705
  • Matching level: species

doi: 10.13145/bacdive159805.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus paeoniae
  • full scientific name: Paenibacillus paeoniae Yan and Tuo 2018

@ref: 66752

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus paeoniae

strain designation: M4BSY-1

type strain: yes

Morphology

cell morphology

  • @ref: 66752
  • gram stain: positive
  • cell length: 2.0-9.0 µm
  • cell width: 0.7-1.2 µm
  • cell shape: rod-shaped

colony morphology

  • @ref: 66752
  • colony color: white
  • colony shape: circular
  • incubation period: 2 days
  • medium used: LB (Luria-Bertani) MEDIUM

pigmentation

  • @ref: 66752
  • production: no

Culture and growth conditions

culture medium

  • @ref: 66752
  • name: LB (Luria-Bertani) MEDIUM
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
66752positiveoptimum30mesophilic
66752positivegrowth10-37

culture pH

@refabilitytypepHPH range
66752positivegrowth7-13alkaliphile
66752positiveoptimum7-8

Physiology and metabolism

oxygen tolerance

  • @ref: 66752
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
66752NaClpositivegrowth0-5 %(w/v)
66752NaClnogrowth7-10 %(w/v)

observation

@refobservation
66752MK-7 is the predominant menaquinone.
66752The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified unknown aminophospholipids and unidentified phospholipids.
66752The diagnostic diamino acid in the cell-wall peptidoglycan is meso-diaminopimelic acid.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
667526731melezitose-builds acid from
6675213643glycol-builds acid from
6675215443inulin-builds acid from
6675215824D-fructose-builds acid from
6675215963ribitol-builds acid from
6675216024D-mannose-builds acid from
6675216443D-tagatose-builds acid from
6675216634raffinose-builds acid from
6675216813galactitol-builds acid from
6675217108D-arabinose-builds acid from
6675217113erythritol-builds acid from
6675217151xylitol-builds acid from
6675217266L-sorbose-builds acid from
6675217268myo-inositol-builds acid from
6675217716lactose-builds acid from
6675217992sucrose-builds acid from
6675218287L-fucose-builds acid from
6675222605arabinitol-builds acid from
6675224265gluconate-builds acid from
6675228053melibiose-builds acid from
6675228847D-fucose-builds acid from
6675230849L-arabinose-builds acid from
6675230911sorbitol-builds acid from
6675232528turanose-builds acid from
6675259640N-acetylglucosamine-builds acid from
6675262318D-lyxose-builds acid from
6675262345L-rhamnose-builds acid from
6675265327D-xylose-builds acid from
6675265328L-xylose-builds acid from
6675274863methyl beta-D-xylopyranoside-builds acid from
66752320061methyl alpha-D-glucopyranoside-builds acid from
667524853esculin+builds acid from
6675212936D-galactose+builds acid from
6675216988D-ribose+builds acid from
6675217057cellobiose+builds acid from
6675217306maltose+builds acid from
6675217634D-glucose+builds acid from
6675217814salicin+builds acid from
6675218305arbutin+builds acid from
6675227082trehalose+builds acid from
6675227613amygdalin+builds acid from
6675228017starch+builds acid from
6675228066gentiobiose+builds acid from
6675228087glycogen+builds acid from
6675229864mannitol+builds acid from
6675217234glucose-fermentation
667525291gelatin-hydrolysis
6675253426tween 80-hydrolysis
66752casein-hydrolysis
6675228017starch+hydrolysis
6675253423tween 40+hydrolysis
6675253424tween 20+hydrolysis

metabolite production

  • @ref: 66752
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

metabolite tests

  • @ref: 66752
  • Chebi-ID: 17234
  • metabolite: glucose
  • methylred-test: -

enzymes

@refvalueactivityec
66752acid phosphatase-3.1.3.2
66752alkaline phosphatase-3.1.3.1
66752alpha-chymotrypsin-3.4.21.1
66752alpha-fucosidase-3.2.1.51
66752alpha-glucosidase-3.2.1.20
66752alpha-mannosidase-3.2.1.24
66752beta-galactosidase-3.2.1.23
66752beta-glucosidase-3.2.1.21
66752beta-glucuronidase-3.2.1.31
66752cystine arylamidase-3.4.11.3
66752esterase-
66752esterase Lipase (C 8)-
66752lipase-
66752N-acetyl-beta-glucosaminidase-3.2.1.52
66752trypsin-3.4.21.4
66752valine arylamidase-
66752alpha-galactosidase+3.2.1.22
66752catalase+1.11.1.6
66752cytochrome oxidase+1.9.3.1
66752leucine arylamidase+3.4.11.1
66752naphthol-AS-BI-phosphohydrolase+

Isolation, sampling and environmental information

isolation

  • @ref: 66752
  • sample type: a surfacesterilized leaf of Paeonia lactiflora Pall
  • host species: Paeonia lactiflora
  • geographic location: Guizhou
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 29.1292
  • longitude: 107.636
  • enrichment culture: ISP 2
  • enrichment culture composition: containing, per 1 l distilled water: 4.0 g yeast extract, 5.0 g malt extract, 4.0 g glucose, 20.0 g agar; pH 7.0
  • enrichment culture duration: 4 weeks
  • enrichment culture temperature: 28
  • isolation procedure: After being dried in a laminar-flow hood, the surface-sterilized leaf was ground into powder by using a micromill and spread on International Streptomyces Project (ISP) 2 agar

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Plant#Leaf (Phyllosphere)
#Host#Plants#Shrub (Scrub)
#Engineered#Treatment#Sterilized (Desinfected)

Sequence information

16S sequences

  • @ref: 66752
  • description: Paenibacillus sp. strain M4BSY-1 16S ribosomal RNA gene, partial sequence
  • accession: MH714913
  • length: 1516
  • database: ena
  • NCBI tax ID: 2292705

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Paenibacillus paeoniae M4BSY-1GCA_003388735contigncbi2292705
66792Paenibacillus paeoniae M4BSY-12904108052draftimg2292705

GC content

  • @ref: 66752
  • GC-content: 48.8
  • method: genome sequence analysis

External links

@ref: 66752

culture collection no.: KCTC 33997, CGMCC 1.13667

literature

  • topic: Phylogeny
  • Pubmed-ID: 30252640
  • title: Paenibacillus paeoniae sp. nov., a novel endophytic bacterium isolated from leaf of Paeonia lactiflora Pall.
  • authors: Yan XR, Tuo L
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003042
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Endophytes/classification/genetics/isolation & purification, Fatty Acids/chemistry, Paenibacillus/*classification/genetics/isolation & purification, Paeonia/*microbiology, Phospholipids/chemistry, *Phylogeny, Plant Leaves/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Enzymology

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66752Xiao-Rui Yan and Li TuoPaenibacillus paeoniae sp. nov., a novel endophytic bacterium isolated from leaf of Paeonia lactiflora Pall.10.1099/ijsem.0.003042IJSEM 68: 3606-3610 201830252640
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes