Strain identifier

BacDive ID: 159793

Type strain: Yes

Species: Brumimicrobium salinarum

Strain Designation: LHR20

NCBI tax ID(s): 2058658 (species)

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General

@ref: 66719

BacDive-ID: 159793

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Brumimicrobium salinarum LHR20 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from sediment sample from marine solar saltern.

NCBI tax id

  • NCBI tax id: 2058658
  • Matching level: species

doi: 10.13145/bacdive159793.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Crocinitomicaceae
  • genus: Brumimicrobium
  • species: Brumimicrobium salinarum
  • full scientific name: Brumimicrobium salinarum Luo et al. 2018

@ref: 66719

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Crocinitomicaceae

genus: Brumimicrobium

species: Brumimicrobium salinarum

strain designation: LHR20

type strain: yes

Morphology

cell morphology

  • @ref: 66719
  • gram stain: negative
  • cell length: 1.5-2.5 µm
  • cell width: 0.3-0.4 µm
  • cell shape: rod-shaped

colony morphology

  • @ref: 66719
  • colony size: 0.5-1 mm
  • colony color: orange
  • colony shape: circular
  • incubation period: 2 days
  • medium used: Marine agar (MA)

Culture and growth conditions

culture medium

  • @ref: 66719
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
66719positivegrowth15-40
66719positiveoptimum33-37mesophilic

culture pH

@refabilitytypepHPH range
66719positivegrowth6.5-9.5alkaliphile
66719positiveoptimum7.5-8.0

Physiology and metabolism

oxygen tolerance

  • @ref: 66719
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
66719NaClpositivegrowth2-11 %(w/v)
66719NaClpositiveoptimum4-5 %(w/v)

observation

@refobservation
66719The respiratory quinone is MK-6.
66719The major polar lipids include phosphatidylethanolamine (PE), aminolipid (AL), glycolipid (GL1) and two unidentified lipids (L1, L2).

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6671916988D-ribose+builds acid from
66719potassium 5-dehydro-D-gluconate+builds acid from
6671915824D-fructose+carbon source
6671916443D-tagatose+carbon source
6671917266L-sorbose+carbon source
6671922599arabinose+carbon source
6671932528turanose+carbon source
6671953258sodium citrate+carbon source
6671962318D-lyxose+carbon source
6671965327D-xylose+carbon source
6671965328L-xylose+carbon source
6671915824D-fructose+energy source
6671916443D-tagatose+energy source
6671917266L-sorbose+energy source
6671922599arabinose+energy source
6671932528turanose+energy source
6671962318D-lyxose+energy source
6671965327D-xylose+energy source
6671965328L-xylose+energy source
667192509agar-hydrolysis
6671953311sodium alginate-hydrolysis
6671953423tween 40-hydrolysis
6671953425tween 60-hydrolysis
66719crystalline cellulose-hydrolysis
667195291gelatin+hydrolysis
6671928017starch+hydrolysis
6671953424tween 20+hydrolysis
6671953426tween 80+hydrolysis

enzymes

@refvalueactivityec
667196-phospho-beta-galactosidase-3.2.1.85
66719alpha-chymotrypsin-3.4.21.1
66719alpha-fucosidase-3.2.1.51
66719alpha-galactosidase-3.2.1.22
66719alpha-mannosidase-3.2.1.24
66719beta-glucosidase-3.2.1.21
66719beta-glucuronidase-3.2.1.31
66719gelatinase-
66719lipase (C 14)-
66719N-acetyl-beta-glucosaminidase-3.2.1.52
66719naphthol-AS-BI-phosphohydrolase-
66719nitrate reductase-1.7.99.4
66719acid phosphatase+3.1.3.2
66719alkaline phosphatase+3.1.3.1
66719catalase+1.11.1.6
66719cystine arylamidase+3.4.11.3
66719cytochrome oxidase+1.9.3.1
66719esterase (C 4)+
66719esterase Lipase (C 8)+
66719leucine arylamidase+3.4.11.1
66719trypsin+3.4.21.4
66719valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66719C15:0 iso70
    66719C15:1 iso4.4
  • type of FA analysis: whole cell analysis
  • incubation medium: MB
  • incubation temperature: 33
  • incubation time: 2
  • system: MIS MIDI
  • method/protocol: Sasser, 2001

Isolation, sampling and environmental information

isolation

  • @ref: 66719
  • sample type: sediment sample from marine solar saltern
  • geographic location: Wendeng
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 36.9
  • longitude: 122.233
  • enrichment culture composition: The medium consisted of the following ingredients in 1000 ml seawater: 0.1?% NH4Cl, 0.2?% CH3COONa, 0.02?% MgSO4?.?7H2O, 0.02?% yeast extract, 0.02?% peptone. The pH of the medium was adjusted to pH 7.5 and then autoclaved.
  • enrichment culture duration: 12 days
  • enrichment culture temperature: 25
  • isolation procedure: After enrichment incubation at 25 C for 12 days, the enriched culture was serially diluted to 10^-5 in sterilized seawater, and samples of each dilution were spread on marine agar 2216 plates.

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment
#Condition#Saline

Sequence information

16S sequences

  • @ref: 66719
  • description: Brumimicrobium sp. LHR20 16S ribosomal RNA gene, partial sequence
  • accession: MG978989
  • length: 1490
  • database: ena
  • NCBI tax ID: 2058658

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brumimicrobium salinarum LHR20GCA_002844555contigncbi2058658
66792Brumimicrobium salinarum LHR202831918959draftimg2058658

GC content

  • @ref: 66719
  • GC-content: 35
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 66719

culture collection no.: KCTC 62372T, MCCC 1H00247

literature

  • topic: Phylogeny
  • Pubmed-ID: 30222094
  • title: Brumimicrobium salinarum sp. nov., isolated from a marine solar saltern.
  • authors: Luo HR, Chen ZY, Fei JJ, Du ZJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003003
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/genetics/isolation & purification, Glycolipids/chemistry, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Salinity, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, *Water Microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66719Hao-Rui Luo, Zi-Yue Chen, Jun-Jie Fei and Zong-Jun DuBrumimicrobium salinarum sp. nov., isolated from a marine solar saltern10.1099/ijsem.0.003003IJSEM 68: 3447-3451 201830222094
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes