Strain identifier

BacDive ID: 159792

Type strain: Yes

Species: Chryseobacterium aurantiacum

Strain Designation: F30

NCBI tax ID(s): 2116499 (species)

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General

@ref: 66706

BacDive-ID: 159792

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming, pigmented

description: Chryseobacterium aurantiacum F30 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and has a orange pigmentation.

NCBI tax id

  • NCBI tax id: 2116499
  • Matching level: species

doi: 10.13145/bacdive159792.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Weeksellaceae
  • genus: Chryseobacterium
  • species: Chryseobacterium aurantiacum
  • full scientific name: Chryseobacterium aurantiacum Luo et al. 2018

@ref: 66706

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Weeksellaceae

genus: Chryseobacterium

species: Chryseobacterium aurantiacum

strain designation: F30

type strain: yes

Morphology

cell morphology

  • @ref: 66706
  • gram stain: negative
  • cell length: 1.0-2.0 µm
  • cell width: 0.2-0.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 66706
  • colony size: 1-2 mm
  • colony color: orange
  • colony shape: circular
  • incubation period: 3 days
  • medium used: LB (Luria-Bertani) MEDIUM

pigmentation

  • @ref: 66706
  • production: yes
  • color: orange
  • name: flexrubin-type pigment

Culture and growth conditions

culture medium

  • @ref: 66706
  • name: LB (Luria-Bertani) MEDIUM
  • growth: yes
  • composition: LB, containing (1 l double distilled water): 10.0 g tryptone (Becton Dickinson, BD), 5.0 g yeast extract (BD), 10.0 g NaCl (Sinopharm Chemical Reagent), 15.0 g agar (Sinopharm Chemical Reagent) for solid plates; pH 7.0]

culture temp

@refgrowthtypetemperaturerange
66706positiveoptimum28mesophilic
66706positivegrowth10-35

culture pH

@refabilitytypepHPH range
66706positiveoptimum6.5
66706positivegrowth5-9alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 66706
  • oxygen tolerance: aerobe

spore formation

  • @ref: 66706
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
66706NaClpositivegrowth0.5-3.5 %(w/v)
66706NaClpositiveoptimum1.5-2.0 %(w/v)

observation

@refobservation
66706The sole respiratory quinone detected is MK-6.
66706The polar lipid profile is phosphatidylethanolamine, two unidentified aminolipids, two unidentified lipids and two unidentified glycolipids

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6670612936D-galactose-assimilation
6670615729L-ornithine-assimilation
6670615740formate-assimilation
6670615792malonate-assimilation
6670615824D-fructose-assimilation
6670616024D-mannose-assimilation
6670616136hydrogen sulfide-assimilation
6670616383cis-aconitate-assimilation
6670616449dl-alanine-assimilation
6670616523D-serine-assimilation
6670616634raffinose-assimilation
6670616704uridine-assimilation
6670617151xylitol-assimilation
6670617203L-proline-assimilation
6670617306maltose-assimilation
6670617596inosine-assimilation
6670617634D-glucose-assimilation
6670617754glycerol-assimilation
6670617992sucrose-assimilation
6670618287L-fucose-assimilation
6670618333D-arabitol-assimilation
6670621217L-alaninamide-assimilation
6670624996lactate-assimilation
6670626490quinate-assimilation
6670627082trehalose-assimilation
6670627605D-psicose-assimilation
6670628066gentiobiose-assimilation
6670628087glycogen-assimilation
6670630031succinate-assimilation
6670630612D-glucarate-assimilation
6670632528turanose-assimilation
6670635581indole-assimilation
6670640585alpha-cyclodextrin-assimilation
6670641865sebacic acid-assimilation
6670653258citric acid-assimilation
66706320055methyl beta-D-glucopyranoside-assimilation
667066359lactulose+assimilation
6670615361pyruvate+assimilation
6670615963ribitol+assimilation
6670615971L-histidine+assimilation
66706167632-oxobutanoate+assimilation
6670616857L-threonine+assimilation
6670616899D-mannitol+assimilation
6670617057cellobiose+assimilation
6670617115L-serine+assimilation
6670617148putrescine+assimilation
6670617196L-asparagine+assimilation
6670617924D-sorbitol+assimilation
6670628053melibiose+assimilation
6670628800N-acetylgalactosamine+assimilation
6670629991L-aspartate+assimilation
6670630031succinate+assimilation
6670630849L-arabinose+assimilation
66706391504-oxopentanoate+assimilation
6670653258sodium citrate+assimilation
6670659640N-acetylglucosamine+assimilation
6670662345L-rhamnose+assimilation
667066731melezitose-builds acid from
6670612936D-galactose-builds acid from
6670615443inulin-builds acid from
6670615824D-fructose-builds acid from
6670615963ribitol-builds acid from
6670616024D-mannose-builds acid from
6670616443D-tagatose-builds acid from
6670616634raffinose-builds acid from
6670616813galactitol-builds acid from
6670617057cellobiose-builds acid from
6670617113erythritol-builds acid from
6670617151xylitol-builds acid from
6670617266L-sorbose-builds acid from
6670617268myo-inositol-builds acid from
6670617716lactose-builds acid from
6670617754glycerol-builds acid from
6670617814salicin-builds acid from
6670617992sucrose-builds acid from
6670618222xylose-builds acid from
6670622599arabinose-builds acid from
6670622605arabinitol-builds acid from
6670627613amygdalin-builds acid from
6670628017starch-builds acid from
6670628053melibiose-builds acid from
6670628066gentiobiose-builds acid from
6670628847D-fucose-builds acid from
6670629864mannitol-builds acid from
6670633942ribose-builds acid from
6670643943methyl alpha-D-mannoside-builds acid from
6670659640N-acetylglucosamine-builds acid from
6670662318D-lyxose-builds acid from
6670662345L-rhamnose-builds acid from
66706320055methyl beta-D-glucopyranoside-builds acid from
66706320061methyl alpha-D-glucopyranoside-builds acid from
667064853esculin+builds acid from
6670616236ethanol+builds acid from
6670617057cellobiose+builds acid from
6670617306maltose+builds acid from
6670617634D-glucose+builds acid from
6670627082trehalose+builds acid from
6670630911sorbitol+builds acid from
6670662345L-rhamnose+builds acid from
6670665327D-xylose+builds acid from
6670653423tween 40-hydrolysis
6670653426tween 80-hydrolysis
6670618186tyrosine+hydrolysis
6670628017starch+hydrolysis
6670653424tween 20+hydrolysis
66706casein+hydrolysis
6670616301nitrite-reduction
6670617632nitrate-reduction

metabolite production

  • @ref: 66706
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

enzymes

@refvalueactivityec
667066-phospho-beta-galactosidase-3.2.1.85
66706alpha-chymotrypsin-3.4.21.1
66706alpha-fucosidase-3.2.1.51
66706alpha-galactosidase-3.2.1.22
66706alpha-mannosidase-3.2.1.24
66706arginine dihydrolase-3.5.3.6
66706beta-glucosidase-3.2.1.21
66706beta-glucuronidase-3.2.1.31
66706lysine decarboxylase-4.1.1.18
66706ornithine decarboxylase-4.1.1.17
66706acid phosphatase+3.1.3.2
66706alkaline phosphatase+3.1.3.1
66706alpha-glucosidase+3.2.1.20
66706catalase+1.11.1.6
66706cystine aminopeptidase+3.4.11.3
66706cytochrome oxidase+1.9.3.1
66706esterase (C 4)+
66706esterase Lipase (C 8)+
66706gelatinase+
66706leucine aminopeptidase+3.4.1.1
66706N-acetyl-beta-glucosaminidase+3.2.1.52
66706naphthol-AS-BI-phosphohydrolase+
66706trypsin+3.4.21.4
66706tryptophan deaminase+4.1.99.1
66706urease+3.5.1.5
66706valine aminopeptidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66706C17:1 w9c25
    66706C15:0 iso30
    66706C17:0 iso 3OH19.6
    66706C16:1 w7c8.5
    66706C15:0 iso 2OH8.5
  • type of FA analysis: whole cell analysis
  • incubation medium: LB-medium
  • incubation temperature: 28
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 66706
  • sample type: from fresh water of a diseased farmed Murray cod pond
  • host species: Murray cod
  • sampling date: 2017-04
  • geographic location: Dajing town, Yueqing City, Zhejiang province PR
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 28.6833
  • longitude: 121.233
  • enrichment culture: Columbia agar (Oxoid)
  • enrichment culture composition: with 5 % sheep blood
  • enrichment culture duration: 3 days
  • enrichment culture temperature: 28
  • isolation procedure: 1 ml sample of water was serially diluted and spread onto Columbia agar (Oxoid) with 5 % sheep blood, and aerobic incubation was performed at 28° C for three days

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Pond (small)
#Infection#Disease
#Host#Fishes

Sequence information

16S sequences

  • @ref: 66706
  • description: Chryseobacterium sp. strain F30 16S ribosomal RNA gene, partial sequence
  • accession: MH041153
  • length: 1482
  • database: ena
  • NCBI tax ID: 2116499

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Chryseobacterium aurantiacum F30GCA_003020585contigncbi2116499
66792Chryseobacterium aurantiacum F302871694252draftimg2116499

GC content

  • @ref: 66706
  • GC-content: 36.3

External links

@ref: 66706

culture collection no.: KCTC 62135, MCCC 1K03457

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny30272541Chryseobacterium aurantiacum sp. nov., isolated from a freshwater pond used for Murray cod (Maccullochella peelii peelii) culture.Luo T, Liu Y, Chen C, Luo Q, Rao Q, Huang M, Tu J, Lin Q, Weng BInt J Syst Evol Microbiol10.1099/ijsem.0.0029872018Animals, Aquaculture, Bacterial Typing Techniques, Base Composition, China, Chryseobacterium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Perciformes, *Phylogeny, Pigmentation, Ponds/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, *Water MicrobiologyTranscriptome
Phylogeny32515727Chryseobacterium vaccae sp. nov., isolated from raw cow's milk.Lee JE, Yoon SH, Lee GY, Lee DH, Huh CS, Kim GBInt J Syst Evol Microbiol10.1099/ijsem.0.0042502020Animals, Bacterial Typing Techniques, Base Composition, Cattle, Chryseobacterium/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Milk/*microbiology, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66706Tuyan Luo, Yang Liu, Can Chen, Qin Luo, Qiuhua Rao, Minmin Huang, Jiefeng Tu, Qiu Lin, Boqi WengChryseobacterium aurantiacum sp. nov., isolated from a freshwater pond used for Murray cod (Maccullochella peelii peelii) culture10.1099/ijsem.0.002987IJSEM 68: 3397-3403 201830272541
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes