Strain identifier

BacDive ID: 159783

Type strain: Yes

Species: Rheinheimera coerulea

Strain Designation: TAPG2

Strain history: <- Wen-Ming Chen, National Kaohsiung Marine Univ.

NCBI tax ID(s): 1908522 (species)

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General

@ref: 66591

BacDive-ID: 159783

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Rheinheimera coerulea TAPG2 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from the freshwater of the Wanan Creek in the vicinity of Pingtung County.

NCBI tax id

  • NCBI tax id: 1908522
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Wen-Ming Chen, National Kaohsiung Marine Univ.

doi: 10.13145/bacdive159783.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Chromatiales
  • family: Chromatiaceae
  • genus: Rheinheimera
  • species: Rheinheimera coerulea
  • full scientific name: Rheinheimera coerulea Sheu et al. 2018

@ref: 66591

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Chromatiales

family: Chromatiaceae

genus: Rheinheimera

species: Rheinheimera coerulea

strain designation: TAPG2

type strain: yes

Morphology

cell morphology

  • @ref: 66591
  • gram stain: negative
  • cell length: 1.0-1.2 µm
  • cell width: 0.6-0.8 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: polar

colony morphology

  • @ref: 66591
  • colony size: 2-3 mm
  • colony color: cream
  • colony shape: circular
  • incubation period: 2 days
  • medium used: Reasoner's 2A agar (R2A)

Culture and growth conditions

culture medium

@refnamegrowth
66591LB (Luria-Bertani) agaryes
66591Nutrient agar (NA)yes
66591Reasoner's 2A agar (R2A)yes
66591trypticase soy agaryes

culture temp

@refgrowthtypetemperaturerange
66591positivegrowth15-37
66591positiveoptimum25-30mesophilic
67771positivegrowth25mesophilic

culture pH

@refabilitytypepH
66591positiveoptimum7
66591positivegrowth6.5-8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
66591aerobe
67771aerobe

spore formation

  • @ref: 66591
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
66591NaClpositivegrowth0-1 %
66591NaClpositiveoptimum0.5 %

observation

@refobservation
66591Cells are surrounded by a thick capsule.
66591Cells are cream-pigmented, becoming dark-green with ongoing incubation (>3 days).
66591The only isoprenoid quinone is Q-8.
66591The polar lipid profile consists of phosphatidylethanolamine, phosphatidylglycerol, an uncharacterized aminophospholipid, an uncharacterized phospholipid, an uncharacterized aminolipid and an uncharacterized lipid.
66591The major polyamine is putrescine.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6659153424tween 20-hydrolysis
6659185146carboxymethylcellulose-hydrolysis
66591corn oil-hydrolysis
6659116991dna+hydrolysis
6659117029chitin+hydrolysis
6659128017starch+hydrolysis
6659153423tween 40+hydrolysis
6659153425tween 60+hydrolysis
6659153426tween 80+hydrolysis
66591casein+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.group IDis resistantresistance conc.
6659128077rifampicinyesyes5 µg (disc)
6659117833gentamicinyesyes10 µg (disc)
6659127902tetracyclineyesyes30 µg (disc)
6659128368novobiocinyesyes30 µg (disc)
66591100147nalidixic acidyesyes30 µg (disc)
6659117698chloramphenicolyesyes30 µg (disc)
665916104kanamycinyesyes30 µg (disc)
6659117076streptomycinyesyes10 µg (disc)
6659145924trimethoprimyesyes1.25 µg (disc)26
665919332sulfamethoxazoleyesyes23.75 µg (disc)26
6659118208penicillin gyesyes10 Unit (disc)

enzymes

@refvalueactivityec
66591catalase+1.11.1.6
66591cytochrome oxidase+1.9.3.1

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66591C14:01.9
    66591C18:01.9
    66591C18:1 w7c12.2
    66591C16:014.4
    66591C15:1 w8c2.6
    66591C17:02.7
    66591C14:0 anteiso2.8
    66591C11:0 3OH2.8
    66591C12:02.8
    66591C16:1 w6c and/or C16:1 w7c34.9
    66591C17:1 w8c7.2
    66591C12:0 3OH8.8
  • type of FA analysis: whole cell analysis
  • incubation medium: Reasoner's 2A agar
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • library/peak naming table: RTSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentlatitudelongitudeenrichment culture durationenrichment culture temperatureisolation dateisolation proceduregeographic location
66591the freshwater of the Wanan Creek in the vicinity of Pingtung CountyTaiwanTWNAsia22.6225120.6473 days2511 October 2015The 200 ml water sample was collected in a sterile bag (PolySilk, 400 ml, Scharlab), stored at 4°C and transported to the laboratory within 6 h, and then it was spread on R2A agar (BD Difco) plates by the standard dilution plating method.
67771From riverTaiwanTWNAsiaPingtung County

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #River (Creek)

Sequence information

16S sequences

  • @ref: 66591
  • description: Rheinheimera sp. TAPG2 partial 16S rRNA gene, strain TAPG2, isolate stream
  • accession: LT627667
  • length: 1420
  • database: ena
  • NCBI tax ID: 1908522

GC content

  • @ref: 66591
  • GC-content: 53.6
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 66591

culture collection no.: BCRC 81054, LMG 30056, KCTC 52815

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny29799388Rheinheimera coerulea sp. nov., isolated from a freshwater creek, and emended description of genus Rheinheimera Brettar et al. 2002.Sheu SY, Chen WT, Young CC, Chen WMInt J Syst Evol Microbiol10.1099/ijsem.0.0028382018Bacterial Typing Techniques, Base Composition, Chromatiaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fresh Water/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Rivers/microbiology, Sequence Analysis, DNA, Taiwan, Ubiquinone/chemistryTranscriptome
Phylogeny31800389Rheinheimera sediminis sp. nov., a marine bacterium isolated from coastal sediment.Yu Q, Qi Y, Zhang H, Pu JInt J Syst Evol Microbiol10.1099/ijsem.0.0039172020Bacterial Typing Techniques, Base Composition, China, Chromatiaceae/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistryTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66591Shih-Yi Sheu, Wei-Ting Chen, Chiu-Chung Young, Wen-Ming ChenRheinheimera coerulea sp. nov., isolated from a freshwater creek, and emended description of genus Rheinheimera Brettar et al. 200210.1099/ijsem.0.002838IJSEM 68: 2340-2347 201829799388
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc