Strain identifier

BacDive ID: 159780

Type strain: Yes

Species: Tsuneonella flava

Strain Designation: MS1-4

NCBI tax ID(s): 2055955 (species)

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General

@ref: 66583

BacDive-ID: 159780

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped

description: Tsuneonella flava MS1-4 is a mesophilic, Gram-negative, rod-shaped bacterium that was isolated from mangrove sediment of the Jiulong River Estuary .

NCBI tax id

  • NCBI tax id: 2055955
  • Matching level: species

doi: 10.13145/bacdive159780.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Sphingomonadales
  • family: Erythrobacteraceae
  • genus: Tsuneonella
  • species: Tsuneonella flava
  • full scientific name: Tsuneonella flava (Ma et al. 2018) Xu et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Altererythrobacter flavus

@ref: 66583

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Sphingomonadales

family: Erythrobacteraceae

genus: Altererythrobacter

species: Altererythrobacter flavus

strain designation: MS1-4

type strain: yes

Morphology

cell morphology

  • @ref: 66583
  • gram stain: negative
  • cell length: 2.0-2.5 µm
  • cell width: 0.3-0.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 66583
  • colony color: yellow
  • medium used: ZB 2216E medium

pigmentation

  • @ref: 66583
  • production: yes
  • name: carotenoid-like pigments with maximum absorption at 448 and 475 nm

Culture and growth conditions

culture medium

  • @ref: 66583
  • name: ZB 2216E medium
  • growth: yes
  • composition: 5 g peptone, 1 g yeast extract, 10 mg ferric phosphate, 15 g agar, 750 ml filtered aged seawater and 250 ml distilled water, pH 7.2

culture temp

@refgrowthtypetemperaturerange
66583positiveoptimum34mesophilic
66583positivegrowth13-40

culture pH

@refabilitytypepHPH range
66583positiveoptimum6
66583positivegrowth4.5-9.0alkaliphile

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
66583NaClpositivegrowth0-6 %(w/v)
66583NaClpositivegrowth4 %(w/v)

observation

@refobservation
66583The sole respiratory quinone is ubiquinone-10 (Q-10).
66583The main polar lipids are diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and glycolipids (GLs).

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6658317268myo-inositol-assimilation
6658317992sucrose-assimilation
6658318401phenylacetate-assimilation
6658325115malate-assimilation
6658327613amygdalin-assimilation
6658327689decanoate-assimilation
6658330911sorbitol-assimilation
6658359640N-acetylglucosamine-assimilation
6658362345L-rhamnose-assimilation
665834-nitrophenyl beta-D-galactopyranoside hydrolysate-assimilation
6658317128adipate+assimilation
6658317234glucose+assimilation
6658317306maltose+assimilation
6658322599arabinose+assimilation
6658324265gluconate+assimilation
6658328053melibiose+assimilation
6658329864mannitol+assimilation
6658337684mannose+assimilation
6658353258citric acid+assimilation
665833557154-nitrophenyl beta-D-galactopyranoside+assimilation
6658317634D-glucose-fermentation
6658318257ornithine-hydrolysis
6658325094lysine-hydrolysis
6658327897tryptophan-hydrolysis
665834853esculin+hydrolysis
665835291gelatin+hydrolysis
6658316199urea+hydrolysis
6658329016arginine+hydrolysis
6658317632nitrate-reduction

metabolite production

  • @ref: 66583
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

enzymes

@refvalueactivityec
66583beta-galactosidase-3.2.1.23
66583beta-glucosidase-3.2.1.21
66583catalase-1.11.1.6
66583N-acetyl-beta-glucosaminidase-3.2.1.52
66583acid phosphatase+3.1.3.2
66583alkaline phosphatase+3.1.3.1
66583alpha-galactosidase+3.2.1.22
66583alpha-glucosidase+3.2.1.20
66583alpha-mannosidase+3.2.1.24
66583beta-D-fucosidase+3.2.1.38
66583beta-glucuronidase+3.2.1.31
66583chymotrypsin+3.4.4.5
66583cystine aminopeptidase+3.4.11.3
66583cytochrome oxidase+1.9.3.1
66583esterase (C 4)+
66583esterase Lipase (C 8)+
66583leucine aminopeptidase+3.4.1.1
66583lipase (C 14)+
66583naphthol-AS-BI-phosphohydrolase+
66583trypsin+3.4.21.4
66583valine aminopeptidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66583C16:1 w5c1
    66583C11:0 iso1.4
    6658311-methyl C18:1 w7c1.8
    66583C18:01.8
    66583C18:1 w5c2.9
    66583C14:0-2OH4.8
    66583C16:1 w7c and/or C16:1 w6c5.1
    66583C16:06.8
    66583C18:1 w7c and/or C18:1 w6c72.9
  • type of FA analysis: whole cell analysis
  • incubation medium: ZB 2216E agar
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 3
  • system: MIS MIDI
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 66583
  • sample type: mangrove sediment of the Jiulong River Estuary (altitude 1 m)
  • geographic location: Fujian Province
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 24.45
  • longitude: 117.9
  • isolation procedure: The sediment sample (5 g) was suspended in isolation medium [200 ml seawater, 2 g NH4NO3, 10 mg FePO4, 1 ml crude oil/diesel oil (1 : 1)] and incubated at 28°C and 150 r.p.m. After incubation for 7 days, 3 ml of a culture was transferred to 20 ml fresh medium. This enrichment procedure was repeated for 4 weeks to obtain oil-degrading microbes. Supernatant from the final culture was diluted 10- to 1000-fold and spread on ZB 2216E medium.

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Estuary
#Environmental#Aquatic#Mangrove
#Environmental#Terrestrial#Sediment
#Host#Plants#Tree

taxonmaps

  • @ref: 69479
  • File name: preview.99_37365.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_20;96_543;97_3937;98_5015;99_37365&stattab=map
  • Last taxonomy: Tsuneonella
  • 16S sequence: KX099616
  • Sequence Identity:
  • Total samples: 147
  • soil counts: 13
  • aquatic counts: 114
  • animal counts: 16
  • plant counts: 4

Sequence information

16S sequences

  • @ref: 66583
  • description: Altererythrobacter flavus strain MS1-4 16S ribosomal RNA gene, partial sequence
  • accession: KX099616
  • length: 1453
  • database: ena
  • NCBI tax ID: 2055955

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Tsuneonella flava MS1-4GCA_002870965contigncbi2055955
66792Tsuneonella flava MS1-42882388624draftimg2055955

GC content

  • @ref: 66583
  • GC-content: 60.40
  • method: genome sequence analysis

External links

@ref: 66583

culture collection no.: MCCC 1K02683, NBRC 112977

literature

  • topic: Phylogeny
  • Pubmed-ID: 33528355
  • title: Tsuneonella suprasediminis sp. nov., isolated from the Pacific Ocean.
  • authors: Gao Y, Li G, Fang C, Shao Z, Wu YH, Xu XW
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.004678
  • year: 2021

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66583Hui Ma, Hao Ren, Lingfeng Huang, Yuanrong LuoAltererythrobacter flavus sp. nov., isolated from mangrove sediment10.1099/ijsem.0.002822IJSEM 68: 2265-2270 201829792590
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/