Strain identifier

BacDive ID: 159725

Type strain: Yes

Species: Kushneria endophytica

Strain Designation: EAod7

NCBI tax ID(s): 2269386 (species)

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General

@ref: 66633

BacDive-ID: 159725

keywords: 16S sequence, Bacteria, aerobe, moderately halophilic, spore-forming, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Kushneria endophytica EAod7 is an aerobe, moderately halophilic, spore-forming bacterium that forms irregular colonies and was isolated from from the aerial part of Arthrocnemum macrostachyum.

NCBI tax id

  • NCBI tax id: 2269386
  • Matching level: species

doi: 10.13145/bacdive159725.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Halomonadaceae
  • genus: Kushneria
  • species: Kushneria endophytica
  • full scientific name: Kushneria endophytica Navarro-Torre et al. 2018

@ref: 66633

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Halomonadaceae

genus: Kushneria

species: Kushneria endophytica

strain designation: EAod7

type strain: yes

Morphology

cell morphology

  • @ref: 66633
  • gram stain: negative
  • cell length: 0.2-0.3 µm
  • cell width: 0.1 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 66633
  • colony size: 2.6 mm
  • colony color: light salmon (RAL 060 80 40)
  • colony shape: irregular
  • incubation period: 2 days
  • medium used: Tryptic Soy Agar

Culture and growth conditions

culture medium

@refnamegrowthcomposition
66633cetrimide agarno
66633MacConkey agaryes
66633Marine agar (MA) 2216yes
66633tryptic soy agar (TSA)yessupplemented with 2.5% NaCl (w/v)

culture temp

@refgrowthtypetemperaturerange
66633positiveoptimum30mesophilic
66633positivegrowth4-37

culture pH

@refabilitytypepH
66633positiveoptimum7
66633positivegrowth5-8

Physiology and metabolism

oxygen tolerance

  • @ref: 66633
  • oxygen tolerance: aerobe

spore formation

  • @ref: 66633
  • type of spore: spore
  • spore formation: yes

halophily

@refhalophily levelsaltgrowthtested relationconcentration
66633moderately halophilicNaClpositivegrowth0.5-25 %(w/v)
66633NaClpositiveminimum0.5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6663316024D-mannose-assimilation
6663317128adipate-assimilation
6663317306maltose-assimilation
6663317634D-glucose-assimilation
6663318401phenylacetate-assimilation
6663327689decanoate-assimilation
6663330849L-arabinose-assimilation
6663353258sodium citrate-assimilation
6663359640N-acetylglucosamine-assimilation
6663316899D-mannitol+assimilation
6663325115malate+assimilation
6663332032potassium gluconate+assimilation
6663315443inulin-builds acid from
6663316634raffinose-builds acid from
6663316899D-mannitol-builds acid from
6663316988D-ribose-builds acid from
6663317716lactose-builds acid from
6663317924D-sorbitol-builds acid from
6663328017starch-builds acid from
6663328087glycogen-builds acid from
6663330849L-arabinose-builds acid from
6663327082trehalose+builds acid from
6663317634D-glucose-fermentation
666335291gelatin-hydrolysis
6663317029chitin-hydrolysis
6663317309pectin-hydrolysis
6663353426tween 80-hydrolysis
6663362968cellulose-hydrolysis
66633casein-hydrolysis
666334853esculin+hydrolysis
6663316991dna+hydrolysis
6663328017starch+hydrolysis
666338391D-gluconate+oxidation
6663312936D-galactose+oxidation
6663315748D-glucuronate+oxidation
6663315824D-fructose+oxidation
6663316024D-mannose+oxidation
6663316537galactarate+oxidation
6663316865gamma-aminobutyric acid+oxidation
6663317115L-serine+oxidation
6663317306maltose+oxidation
6663317309pectin+oxidation
6663317464L-galactonic acid gamma-lactone+oxidation
6663317596inosine+oxidation
6663317634D-glucose+oxidation
6663317754glycerol+oxidation
6663317968butyrate+oxidation
6663317992sucrose+oxidation
6663318024D-galacturonic acid+oxidation
66633181014-hydroxyphenylacetic acid+oxidation
6663318333D-arabitol+oxidation
6663327082trehalose+oxidation
6663328847D-fucose+oxidation
6663329985L-glutamate+oxidation
6663330612D-glucarate+oxidation
6663330797(S)-malic acid+oxidation
6663332323glucuronamide+oxidation
6663332528turanose+oxidation
6663351850methyl pyruvate+oxidation
6663353258citric acid+oxidation
6663362965sodium formate+oxidation
6663370744glycine-proline+oxidation
6663375228sodium lactate+oxidation
6663317632nitrate+reduction

metabolite production

@refChebi-IDmetaboliteproduction
6663315688acetoinno
6663335581indoleno

metabolite tests

  • @ref: 66633
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
66633alpha-fucosidase-3.2.1.51
66633alpha-mannosidase-3.2.1.24
66633arginine dihydrolase-3.5.3.6
66633beta-glucuronidase-3.2.1.31
66633cystine arylamidase-3.4.11.3
66633cytochrome oxidase-1.9.3.1
66633N-acetyl-beta-glucosaminidase-3.2.1.52
66633pyrrolidonyl arylamidase-3.4.19.3
66633trypsin-3.4.21.4
66633urease-3.5.1.5
66633valine arylamidase-
66633acid phosphatase+3.1.3.2
66633alkaline phosphatase+3.1.3.1
66633alpha-chymotrypsin+3.4.21.1
66633alpha-galactosidase+3.2.1.22
66633alpha-glucosidase+3.2.1.20
66633beta-galactosidase+3.2.1.23
66633beta-glucosidase+3.2.1.21
66633catalase+1.11.1.6
66633esterase (C 4)+
66633esterase Lipase (C 8)+
66633leucine aminopeptidase+3.4.1.1
66633leucine arylamidase+3.4.11.1
66633lipase (C 14)+
66633naphthol-AS-BI-phosphohydrolase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66633C16:1 w7c and/or C16:1 w6c13
    66633C16:027
    66633C12:0 2-OH1.4
    66633C12:02.1
    66633C18:1 w7c and/or C18:1 w6c44.6
    66633C12:0 3OH9.2
  • type of FA analysis: whole cell analysis
  • incubation medium: tryptic soy agar with 2.5 % (w/v) NaCl
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA40
  • system: MIS MIDI
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 66633
  • sample type: from the aerial part of Arthrocnemum macrostachyum
  • host species: Arthrocnemum macrostachyum
  • geographic location: Odiel marshes in Huelva
  • country: Spain
  • origin.country: ESP
  • continent: Europe
  • latitude: 37.2166
  • longitude: -6.95
  • enrichment culture: tryptic soy agar
  • enrichment culture composition: supplemented with 0.3 M NaCl
  • enrichment culture duration: 3 days
  • enrichment culture temperature: 28

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Shrub (Scrub)

taxonmaps

  • @ref: 69479
  • File name: preview.99_79496.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_6462;97_7747;98_55416;99_79496&stattab=map
  • Last taxonomy: Kushneria endophytica subclade
  • 16S sequence: KU320860
  • Sequence Identity:
  • Total samples: 3
  • animal counts: 3

Sequence information

16S sequences

  • @ref: 66633
  • description: Kushneria endophytica strain EAod7 16S ribosomal RNA gene, partial sequence
  • accession: KU320860
  • length: 1418
  • database: ena
  • NCBI tax ID: 2269386

External links

@ref: 66633

culture collection no.: CECT 9075, LMG 29858

literature

  • topic: Phylogeny
  • Pubmed-ID: 30010522
  • title: Kushneria phyllosphaerae sp. nov. and Kushneria endophytica sp. nov., plant growth promoting endophytes isolated from the halophyte plant Arthrocnemum macrostachyum.
  • authors: Navarro-Torre S, Carro L, Rodriguez-Llorente ID, Pajuelo E, Caviedes MA, Igual JM, Redondo-Gomez S, Camacho M, Klenk HP, Montero-Calasanz MDC
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002897
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, Chenopodiaceae/*microbiology, DNA, Bacterial/genetics, Endophytes/classification/isolation & purification, Fatty Acids/chemistry, Halomonadaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salt-Tolerant Plants/*microbiology, Sequence Analysis, DNA, Spain, Ubiquinone/chemistry
  • topic2: Enzymology

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66633Salvadora Navarro-Torre, Lorena Carro, Ignacio D. Rodriguez-Llorente, Eloisa Pajuelo, Miguel Angel Caviedes, Jose Mariano Igual, Susana Redondo-Gomez, Maria Camacho, Hans-Peter Klenk, Maria del Carmen Montero-CalasanzKushneria phyllosphaerae sp. nov. and Kushneria endophytica sp. nov., plant growth promoting endophytes isolated from the halophyte plant Arthrocnemum macrostachyum10.1099/ijsem.0.002897IJSEM 68: 2800-2806 201830010522
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/