Strain identifier
BacDive ID: 159662
Type strain: 
Species: Streptomyces diacarni
Strain Designation: LHW51701
Strain history: <- L. Li, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Dep. Department of Pharmacy, Shanghai, P.R. China; LHW51701
NCBI tax ID(s): 2800381 (species)
version 8.1 (current version)
General
@ref: 66512
BacDive-ID: 159662
DSM-Number: 106126
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic
description: Streptomyces diacarni LHW51701 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from marine sponge.
NCBI tax id
- NCBI tax id: 2800381
- Matching level: species
strain history
- @ref: 66512
- history: <- L. Li, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Dep. Department of Pharmacy, Shanghai, P.R. China; LHW51701
doi: 10.13145/bacdive159662.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces diacarni
- full scientific name: Streptomyces diacarni Li et al. 2019
@ref: 66512
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Streptomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces diacarni
full scientific name: Streptomyces diacarni Li et al. 2019
strain designation: LHW51701
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | no | 92.99 | |
| 69480 | 100 | positive |
colony morphology
| @ref | colony color | medium used |
|---|---|---|
| 69299 | Brown beige (1011) | ISP 5 |
| 69299 | Brown beige (1011) | ISP 7 |
| 69299 | Ivory (1014) | suter without tyrosine |
| 69299 | Light ivory (1015) | ISP 4 |
| 69299 | Olive grey (7002) | ISP 6 |
| 69299 | Signal brown (8002) | ISP 2 |
| 69299 | Signal brown (8002) | ISP 3 |
| 69299 | Signal brown (8002), light ivory (1015) | suter with tyrosine |
multicellular morphology
| @ref | forms multicellular complex | complex name | medium name | complex color |
|---|---|---|---|---|
| 69299 | no | Aerial mycelium | ISP 2 | |
| 69299 | yes | Aerial mycelium | ISP 3 | Signal white (9003) |
| 69299 | no | Aerial mycelium | ISP 4 | |
| 69299 | yes | Aerial mycelium | ISP 5 | Grey white (9002) |
| 69299 | yes | Aerial mycelium | ISP 6 | Grey white (9002) |
| 69299 | yes | Aerial mycelium | ISP 7 | Grey white (9002) |
| 69299 | no | Aerial mycelium | suter with tyrosine | |
| 69299 | no | Aerial mycelium | suter without tyrosine |
pigmentation
| @ref | production | name | color |
|---|---|---|---|
| 69299 | no | Melanin | |
| 69299 | yes | soluble pigment | Ochre brown (8001), oxide red (9003), signal brown (8002), brown beige (1011), copper brown (8004), sand yellow (1002) |
multimedia
- @ref: 66512
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_106126.jpg
- caption: Medium 65 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 66512 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 66512 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://mediadive.dsmz.de/medium/84 | Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water |
| 66512 | STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) | yes | https://mediadive.dsmz.de/medium/252 | Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water |
culture temp
- @ref: 66512
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69480 | yes | 100 |
| 69481 | yes | 100 |
halophily
- @ref: 69299
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-10 %
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 69299 | 22599 | arabinose | + | growth |
| 69299 | 62968 | cellulose | - | growth |
| 69299 | 28757 | fructose | + | growth |
| 69299 | 17234 | glucose | + | growth |
| 69299 | 17268 | inositol | + | growth |
| 69299 | 37684 | mannose | +/- | growth |
| 69299 | 16634 | raffinose | +/- | growth |
| 69299 | 26546 | rhamnose | +/- | growth |
| 69299 | 17992 | sucrose | - | growth |
| 69299 | 18222 | xylose | + | growth |
Isolation, sampling and environmental information
isolation
- @ref: 66512
- sample type: marine sponge
- geographic location: Xisha Islands, Hainan Province (16° 45' N, 112° 21' E)
- country: China
- origin.country: CHN
- continent: Asia
- latitude: 16.75
- longitude: 112.35
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Marine |
| #Host | #Invertebrates (Other) | #Porifera (Sponges) |
Sequence information
16S sequences
- @ref: 66512
- description: Streptomyces sp. strain LHW51701 16S ribosomal RNA gene, partial sequence
- accession: KX347890
- length: 1487
- database: ena
- NCBI tax ID: 1931
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Streptomyces diacarni LHW51701 | GCA_003323715 | scaffold | ncbi | 2800381 |
| 66792 | Candidatus Streptomyces philanthi strain LHW51701 | 371608.5 | wgs | patric | 371608 |
GC content
- @ref: 66512
- GC-content: 72.1
- method: sequence analysis
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| flagellated | no | 97.891 | no |
| gram-positive | yes | 91.545 | no |
| anaerobic | no | 98.889 | no |
| aerobic | yes | 91.694 | no |
| halophile | no | 83.603 | no |
| spore-forming | yes | 94.438 | no |
| glucose-util | yes | 88.552 | yes |
| thermophile | no | 95.068 | yes |
| motile | no | 94.739 | no |
| glucose-ferment | no | 87.552 | no |
External links
@ref: 66512
culture collection no.: DSM 106126, CCTCC AA 2018017
straininfo link
- @ref: 112136
- straininfo: 403738
literature
- topic: Phylogeny
- Pubmed-ID: 30427301
- title: Streptomyces reniochalinae sp. nov. and Streptomyces diacarni sp. nov., from marine sponges.
- authors: Li L, Wang J, Zhou YJ, Lin HW, Lu YH
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.003109
- year: 2018
- mesh: Animals, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Multilocus Sequence Typing, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Porifera/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 66512 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-106126 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106126) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69299 | Wink, J. | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | https://cdn.dsmz.de/wink/DSM%20106126.pdf | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 112136 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID403738.1 |