General

@ref: 66226

BacDive-ID: 159403

DSM-Number: 105285

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, psychrophilic, Gram-negative, coccus-shaped, colony-forming

description: Deinococcus psychrotolerans S14-83 is an obligate aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from soil.

NCBI tax id

• NCBI tax id: 2489213
• Matching level: species

strain history

• @ref: 66226
• history: <- P. Liu, China Center for Type Culture Collection (CCTCC), Wuhan; S14-83 <- Y. Zhao

doi: 10.13145/bacdive159403.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/deinococcota
• domain: Bacteria
• phylum: Deinococcota
• class: Deinococci
• order: Deinococcales
• family: Deinococcaceae
• genus: Deinococcus
• species: Deinococcus psychrotolerans
• full scientific name: Deinococcus psychrotolerans Tian et al. 2019

@ref: 66226

domain: Bacteria

phylum: Deinococcus-Thermus

class: Deinococci

order: Deinococcales

family: Deinococcaceae

genus: Deinococcus

species: Deinococcus psychrotolerans

full scientific name: Deinococcus psychrotolerans Tian et al. 2019

strain designation: S14-83

type strain: yes

Morphology

cell morphology

colony morphology

• @ref: 68141
• colony color: pale red
• colony shape: circular
• incubation period: 5 days
• medium used: Reasoner's 2A agar (R2A)

Culture and growth conditions

culture medium

• @ref: 66226
• name: GLYCEROL-YEAST EXTRACT-PEPTONE MEDIUM (DSMZ Medium 1484)
• growth: yes
• link: https://mediadive.dsmz.de/medium/1484
• composition: Name: Glycerol-Yeast extract-Peptone Medium (DSMZ Medium 1484) Composition: Agar 15.0 g/l Yeast extract 5.0 g/l Bacto peptone 3.0 g/l Betaine 1.25 g/l Sodium pyruvate 1.25 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l ZnSO4 x 7 H2O 0.0018 g/l CoSO4 x 7 H2O 0.0018 g/l FeSO4 x 7 H2O 0.001 g/l CaCl2 x 2 H2O 0.001 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l Pyridoxine hydrochloride 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l H3BO3 0.0001 g/l CuSO4 x 5 H2O 0.0001 g/l (DL)-alpha-Lipoic acid 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l Nicotinic acid 5e-05 g/l Riboflavin 5e-05 g/l Thiamine HCl 5e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Vitamin B12 1e-06 g/l Glycerol Distilled water

culture temp

culture pH

Physiology and metabolism

oxygen tolerance

• @ref: 68141
• oxygen tolerance: obligate aerobe

spore formation

halophily

• @ref: 68141
• salt: NaCl
• growth: positive
• tested relation: growth
• concentration: 0-1 %(w/v)

observation

• @ref: 68141
• observation: occur mostly in pairs and rarely as single cells

metabolite utilization

metabolite production

enzymes

fatty acid profile

• fatty acids

  @ref	fatty acid	percentage
  68141	C14:0	1.3
  68141	C16:0	26.1
  68141	C17:0	0.5
  68141	C18:0	0.6
  68141	C15:1 w6c	1.2
  68141	Summed feature 2	1.2
  68141	C16:1 w7c/C16:1 w6c	55.5
  68141	C16:0 Nalcohol	6.7
  68141	C17:0 cyclo	5.4

• type of FA analysis: whole cell analysis
• incubation medium: R2A
• incubation temperature: 20
• incubation time: 5
• library/peak naming table: TSBA6
• system: MIS MIDI
• method/protocol: Sasser, 1990
• cutoff value:

Isolation, sampling and environmental information

isolation

isolation source categories

• Cat1: #Environmental
• Cat2: #Terrestrial
• Cat3: #Soil

taxonmaps

• @ref: 69479
• File name: preview.99_47339.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4679;96_21490;97_26833;98_34156;99_47339&stattab=map
• Last taxonomy: Deinococcus
• 16S sequence: KX499640
• Sequence Identity:
• Total samples: 402
• soil counts: 110
• aquatic counts: 220
• animal counts: 56
• plant counts: 16

Sequence information

16S sequences

• @ref: 66226
• description: Deinococcus sp. strain S14-83 16S ribosomal RNA gene, partial sequence
• accession: KX499640
• length: 1402
• database: ena
• NCBI tax ID: 47478

Genome sequences

• @ref: 68141
• description: Deinococcus psychrotolerans S14-83
• accession: GCA_003860465
• assembly level: complete
• database: ncbi
• NCBI tax ID: 2489213

GC content

• @ref: 68141
• GC-content: 61.1
• method: genome sequence analysis

Genome-based predictions

predictions

External links

@ref: 66226

culture collection no.: DSM 105285, CCTCC AB 2015449

straininfo link

• @ref: 111898
• straininfo: 407072

literature

• topic: Phylogeny
• Pubmed-ID: 31647398
• title: Deinococcus psychrotolerans sp. nov., isolated from soil on the South Shetland Islands, Antarctica.
• authors: Tian J, Wang L, Liu P, Geng Y, Zhu G, Zheng R, Liu Z, Zhao Y, Yang J, Peng F
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijsem.0.003484
• year: 2019
• mesh: Antarctic Regions, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Deinococcus/*classification/isolation & purification/radiation effects, Fatty Acids/chemistry, Gamma Rays, Glycolipids/chemistry, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ultraviolet Rays, Vitamin K 2/analogs & derivatives/chemistry
• topic2: Transcriptome

Reference