Archive BacDiveID:10.13145/bacdive159198.20230509.8.1

Strain identifier

BacDive ID: 159198

Type strain:

Species: Desulfobotulus mexicanus

Strain Designation: PAR22N

Strain history: A. Hirschler-Réa; MIO, Aix-Marseille Univ., France; PAR22N.

NCBI tax ID(s): 2586642 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 66021

BacDive-ID: 159198

DSM-Number: 105758

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Desulfobotulus mexicanus PAR22N is a mesophilic bacterium that was isolated from sediment of alkaline crater lake.

NCBI tax id

NCBI tax id: 2586642
Matching level: species

strain history

@ref	history
66021	<- A. Hirschler-Réa, Aix-Marseille Univ., France; PAR 22N <- P. Bernal {2016}
67770	A. Hirschler-Réa; MIO, Aix-Marseille Univ., France; PAR22N.

doi: 10.13145/bacdive159198.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Deltaproteobacteria
order: Desulfobacterales
family: Desulfobacteraceae
genus: Desulfobotulus
species: Desulfobotulus mexicanus
full scientific name: Desulfobotulus mexicanus Pérez-Bernal et al. 2020

@ref: 66021

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Desulfobacterales

family: Desulfobacteraceae

genus: Desulfobotulus

species: Desulfobotulus mexicanus

full scientific name: Desulfobotulus mexicanus Perez-Bernal et al. 2020

strain designation: PAR22N

type strain: yes

Culture and growth conditions

culture medium

@ref: 66021
name: DESULFOBULBUS SP. MEDIUM (FRESHWATER) (DSMZ Medium 194)
growth: yes
link: https://mediadive.dsmz.de/medium/194
composition: Name: DESULFOBULBUS SP. MEDIUM (FRESHWATER) (DSMZ Medium 194; with strain-specific modifications) Composition: Na2SO4 2.99103 g/l Na2CO3 1.49551 g/l NaCl 0.997009 g/l Na-butyrate 0.697906 g/l KCl 0.498504 g/l Na2S x 9 H2O 0.398804 g/l MgCl2 x 6 H2O 0.398804 g/l NH4Cl 0.299103 g/l Na-caproate 0.299103 g/l KH2PO4 0.199402 g/l Sodium octanoate 0.149551 g/l CaCl2 x 2 H2O 0.149551 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
66021	positive	growth	30	mesophilic
67770	positive	growth	30	mesophilic

Physiology and metabolism

observation

@ref: 67770
observation: quinones: MK-7

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent	latitude	longitude
66021	sediment of alkaline crater lake	Rincon de Parangueo Lac, Vallee de Santiago, Guanajuato	Mexico	MEX	North America	20.4321	-101.247
67770	Sediment of an alkaline crater lake in Guanajuato		Mexico	MEX	North America

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
66021	Desulfobotulus sp. strain PAR22N 16S ribosomal RNA gene, partial sequence	KY523541.1	658	ena	1940337
67770	Desulfobotulus mexicanus strain PAR22N 16S ribosomal RNA gene, partial sequence	KY523541	1540	ena	2586642

Genome sequences

@ref: 67770
description: Desulfobotulus mexicanus PAR22N
accession: GCA_006175995
assembly level: contig
database: ncbi
NCBI tax ID: 2586642

GC content

@ref	GC-content	method
66021	49	sequence analysis
67770	49	genome sequence analysis

Genome-based predictions

predictions

trait	prediction	confidence	training_data
motile	yes	88.746	no
flagellated	no	69.028	no
gram-positive	no	99.261	no
anaerobic	yes	98.915	no
aerobic	no	96.99	no
halophile	no	55.677	no
spore-forming	no	92.433	no
glucose-util	no	60.934	no
thermophile	no	93.868	no
glucose-ferment	no	85.683	no

External links

@ref: 66021

culture collection no.: DSM 105758, JCM 32146

straininfo link

@ref: 111699
straininfo: 401094

literature

topic: Phylogeny
Pubmed-ID: 32271141
title: Desulfobotulus mexicanus sp. nov., a novel sulfate-reducing bacterium isolated from the sediment of an alkaline crater lake in Mexico.
authors: Perez-Bernal MF, Brito EMS, Bartoli M, Aube J, Ollivier B, Guyoneaud R, Hirschler-Rea A
journal: Int J Syst Evol Microbiol
DOI: 10.1099/ijsem.0.004159
year: 2020
mesh: Alkalies, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Deltaproteobacteria/*classification/isolation & purification, Fatty Acids/chemistry, Geologic Sediments/microbiology, Lakes/*microbiology, Mexico, Oxidation-Reduction, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfates/*metabolism, Sulfur-Reducing Bacteria/classification/isolation & purification
topic2: Enzymology

Reference

@id	authors	title	doi/url	catalogue
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ	10.1099/ijsem.0.004332
66021	Curators of the DSMZ		https://www.dsmz.de/collection/catalogue/details/culture/DSM-105758	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105758)
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
111699	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.	StrainInfo: A central database for resolving microbial strain identifiers	10.60712/SI-ID401094.1