Strain identifier

BacDive ID: 158987

Type strain: No

Species: Limosilactobacillus reuteri

Strain Designation: WCA-386-APC-4I

Strain history: <- T. Clavel; TU Munich, Freising-Weihenstephan, Germany;

NCBI tax ID(s): 1598 (species)

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General

@ref: 65810

BacDive-ID: 158987

DSM-Number: 108836

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Limosilactobacillus reuteri WCA-386-APC-4I is a mesophilic bacterium that was isolated from feces; pig ,4 month old, genotype: APC1311/+ referring to the adenomatous-polyposis-coli protein.

NCBI tax id

  • NCBI tax id: 1598
  • Matching level: species

strain history

  • @ref: 65810
  • history: <- T. Clavel; TU Munich, Freising-Weihenstephan, Germany;

doi: 10.13145/bacdive158987.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Limosilactobacillus
  • species: Limosilactobacillus reuteri
  • full scientific name: Limosilactobacillus reuteri (Kandler et al. 1982) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus reuteri

@ref: 65810

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Limosilactobacillus

species: Limosilactobacillus reuteri

full scientific name: Limosilactobacillus reuteri (Kandler et al. 1982) Zheng et al. 2020

strain designation: WCA-386-APC-4I

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65810FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a)yeshttps://mediadive.dsmz.de/medium/1203aName: FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a) Composition: Fastidious Anaerobe Basal Broth 35.4 g/l Distilled water
65810COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

  • @ref: 65810
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate+reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
65810-+++-+/------+/---+--+-+++-+++/-+-+/-

Isolation, sampling and environmental information

isolation

  • @ref: 65810
  • sample type: feces; pig ,4 month old, genotype: APC1311/+ referring to the adenomatous-polyposis-coli (APC) protein
  • geographic location: Kranzberg (Bavaria)
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Mammals#Suidae (Pig,Swine)

taxonmaps

  • @ref: 69479
  • File name: preview.99_581.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_369;97_416;98_475;99_581&stattab=map
  • Last taxonomy: Limosilactobacillus reuteri
  • 16S sequence: MN537548
  • Sequence Identity:
  • Total samples: 169334
  • soil counts: 4867
  • aquatic counts: 7596
  • animal counts: 154945
  • plant counts: 1926

Safety information

risk assessment

  • @ref: 65810
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 65810
  • description: Lactobacillus reuteri strain DSM 108836 16S ribosomal RNA gene, partial sequence
  • accession: MN537548
  • length: 1536
  • database: ena
  • NCBI tax ID: 1598

Genome sequences

  • @ref: 66792
  • description: Limosilactobacillus reuteri WCA-386-APC-4I
  • accession: GCA_012843635
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1598

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno95.998no
flagellatedno98.844no
gram-positiveyes96.762no
anaerobicno71.527no
aerobicno93.789no
halophileyes83.076no
spore-formingno93.462no
thermophileno99.206yes
glucose-utilyes90.127no
glucose-fermentyes88.711no

External links

@ref: 65810

culture collection no.: DSM 108836

straininfo link

  • @ref: 111503
  • straininfo: 408147

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65810Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-108836Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 108836)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
111503Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID408147.1