Strain identifier
BacDive ID: 158938
Type strain: 
Species: Enterococcus hirae
Strain Designation: WCA-386-APC-3D
Strain history: <- D. Wylensek, TU Munich, Freising-Weihenstephan, Germany; WCA-386-APC-3D
NCBI tax ID(s): 1354 (species)
version 8.1 (current version)
General
@ref: 65761
BacDive-ID: 158938
DSM-Number: 100949
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, human pathogen
description: Enterococcus hirae WCA-386-APC-3D is a mesophilic human pathogen that was isolated from feces; pig, 4 month old, genotype: APC1311/+ referring to the adenomatous-polyposis-coli protein.
NCBI tax id
- NCBI tax id: 1354
- Matching level: species
strain history
- @ref: 65761
- history: <- D. Wylensek, TU Munich, Freising-Weihenstephan, Germany; WCA-386-APC-3D
doi: 10.13145/bacdive158938.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Enterococcaceae
- genus: Enterococcus
- species: Enterococcus hirae
- full scientific name: Enterococcus hirae Farrow and Collins 1985
@ref: 65761
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Enterococcaceae
genus: Enterococcus
species: Enterococcus hirae
full scientific name: Enterococcus hirae Farrow and Collins 1985
strain designation: WCA-386-APC-3D
type strain: no
Culture and growth conditions
culture medium
- @ref: 65761
- name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)
- growth: yes
- link: https://mediadive.dsmz.de/medium/92
- composition: Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
culture temp
- @ref: 65761
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68381 | 29016 | arginine | + | hydrolysis |
| 68381 | 16988 | D-ribose | + | builds acid from |
| 68381 | 16899 | D-mannitol | - | builds acid from |
| 68381 | 30911 | sorbitol | - | builds acid from |
| 68381 | 17716 | lactose | + | builds acid from |
| 68381 | 27082 | trehalose | + | builds acid from |
| 68381 | 16634 | raffinose | + | builds acid from |
| 68381 | 17992 | sucrose | + | builds acid from |
| 68381 | 30849 | L-arabinose | - | builds acid from |
| 68381 | 18333 | D-arabitol | - | builds acid from |
| 68381 | 40585 | alpha-cyclodextrin | + | builds acid from |
| 68381 | 606565 | hippurate | - | hydrolysis |
| 68381 | 28087 | glycogen | - | builds acid from |
| 68381 | 27941 | pullulan | - | builds acid from |
| 68381 | 17306 | maltose | + | builds acid from |
| 68381 | 28053 | melibiose | + | builds acid from |
| 68381 | 6731 | melezitose | - | builds acid from |
| 68381 | 320055 | methyl beta-D-glucopyranoside | + | builds acid from |
| 68381 | 16443 | D-tagatose | - | builds acid from |
| 68381 | 16199 | urea | - | hydrolysis |
metabolite production
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- production: yes
metabolite tests
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: +
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68381 | urease | - | 3.5.1.5 |
| 68381 | beta-mannosidase | - | 3.2.1.25 |
| 68381 | glycyl tryptophan arylamidase | + | |
| 68381 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
| 68381 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
| 68381 | beta-galactosidase | + | 3.2.1.23 |
| 68381 | Alanyl-Phenylalanyl-Proline arylamidase | - | |
| 68381 | alkaline phosphatase | - | 3.1.3.1 |
| 68381 | alpha-galactosidase | + | 3.2.1.22 |
| 68381 | beta-glucuronidase | - | 3.2.1.31 |
| 68381 | beta-glucosidase | + | 3.2.1.21 |
| 68381 | arginine dihydrolase | + | 3.5.3.6 |
API rID32STR
| @ref | ADH Arg | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | CDEX | VP | APPA | beta GAL | PyrA | beta NAG | GTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Mbeta DG | TAG | beta MAN | URE |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 65761 | + | + | + | - | + | - | + | - | - | + | + | + | + | - | - | + | + | - | + | + | + | + | - | - | - | + | + | - | + | - | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 65761
- sample type: feces; pig, 4 month old, genotype: APC1311/+ referring to the adenomatous-polyposis-coli (APC) protein
- geographic location: Kranzberg (Bavaria)
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
| #Host | #Mammals | #Suidae (Pig,Swine) |
taxonmaps
- @ref: 69479
- File name: preview.99_23.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_19;97_19;98_22;99_23&stattab=map
- Last taxonomy: Enterococcus
- 16S sequence: MN537443
- Sequence Identity:
- Total samples: 55544
- soil counts: 1007
- aquatic counts: 2532
- animal counts: 51351
- plant counts: 654
Safety information
risk assessment
- @ref: 65761
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 65761
- description: Enterococcus sp. strain DSM 100949 16S ribosomal RNA gene, partial sequence
- accession: MN537443
- length: 1513
- database: ena
- NCBI tax ID: 35783
Genome sequences
- @ref: 66792
- description: Enterococcus hirae WCA-386-APC-3D
- accession: GCA_012843775
- assembly level: contig
- database: ncbi
- NCBI tax ID: 1354
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 95.706 | no |
| flagellated | no | 97.77 | no |
| gram-positive | yes | 94.498 | no |
| anaerobic | no | 94.705 | no |
| aerobic | no | 96.766 | no |
| halophile | yes | 90.958 | no |
| spore-forming | no | 92.402 | no |
| glucose-util | yes | 86.693 | no |
| thermophile | no | 99.751 | no |
| glucose-ferment | yes | 90.028 | no |
External links
@ref: 65761
culture collection no.: DSM 100949
straininfo link
- @ref: 111455
- straininfo: 406830
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 65761 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-100949 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100949) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 68381 | Automatically annotated from API rID32STR | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 111455 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID406830.1 |