Strain identifier

BacDive ID: 158766

Type strain: Yes

Species: Salibacterium lacus

Strain Designation: GSS13

Strain history: <- Yue-qiang Wang, Guangdong Institute of Eco-environment and Soil Science.

NCBI tax ID(s): 1898109 (species)

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General

@ref: 65520

BacDive-ID: 158766

keywords: 16S sequence, Bacteria, aerobe, Gram-positive, motile, rod-shaped, colony-forming

description: Salibacterium lacus GSS13 is an aerobe, Gram-positive, motile bacterium that forms circular colonies and was isolated from sediment of saline lake.

NCBI tax id

  • NCBI tax id: 1898109
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Yue-qiang Wang, Guangdong Institute of Eco-environment and Soil Science.

doi: 10.13145/bacdive158766.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Salibacterium
  • species: Salibacterium lacus
  • full scientific name: Salibacterium lacus Wang et al. 2018

@ref: 65520

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Salibacterium

species: Salibacterium lacus

strain designation: GSS13

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
65520positive1.3-3.5 µm0.6-1.8 µmrod-shapedyesmonopolar
67771rod-shapedyes
67771positive

colony morphology

  • @ref: 65520
  • colony size: 1.0-2.0 mm
  • colony color: white
  • colony shape: circular
  • incubation period: 3 days
  • medium used: LB

Culture and growth conditions

culture medium

@refnamegrowthcomposition
65520LB (Luria-Bertani) MEDIUMyeswith 20 % NaCl
65520modified LByes10 g/l casein tryptone, 5 g/l yeast extract, 200 g/l NaCl

culture temp

@refgrowthtypetemperaturerange
65520positivegrowth10-45
65520positiveoptimum30mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
65520positivegrowth5.5-8.5alkaliphile
65520positiveoptimum7.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
65520aerobe
67771aerobe

spore formation

@refspore formation
65520no
67771no

halophily

@refsaltgrowthtested relationconcentrationhalophily level
65520NaClpositivegrowth8-28 %(w/v)
65520NaClpositiveoptimum24 %(w/v)halophilic

observation

  • @ref: 67771
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6552017634D-glucose+carbon source
6552017634D-glucose+energy source
6552016899D-mannitol+carbon source
6552016899D-mannitol+energy source
6552016988D-ribose-carbon source
6552016988D-ribose-energy source
655204853esculin+hydrolysis
6552028087glycogen-carbon source
6552028087glycogen-energy source
6552017268myo-inositol-carbon source
6552017268myo-inositol-energy source
6552017240itaconate-carbon source
6552017240itaconate-energy source
6552016977L-alanine-carbon source
6552016977L-alanine-energy source
6552018287L-fucose-carbon source
6552018287L-fucose-energy source
6552062345L-rhamnose+carbon source
6552062345L-rhamnose+energy source
6552017115L-serine+carbon source
6552017115L-serine+energy source
6552024996lactate-carbon source
6552024996lactate-energy source
6552017306maltose-carbon source
6552017306maltose-energy source
6552028053melibiose-carbon source
6552028053melibiose-energy source
65520506227N-acetylglucosamine+carbon source
65520506227N-acetylglucosamine+energy source
6552017632nitrate-reduction
65520potassium 5-dehydro-D-gluconate+builds acid from
6552017814salicin-carbon source
6552017814salicin-energy source
6552032954sodium acetate-carbon source
6552032954sodium acetate-energy source
6552030911sorbitol-carbon source
6552030911sorbitol-energy source
655209300suberic acid-carbon source
655209300suberic acid-energy source
6552017992sucrose-carbon source
6552017992sucrose-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6552016136hydrogen sulfideno
6552035581indoleno

enzymes

@refvalueactivityec
65520catalase+1.11.1.6
65520cytochrome oxidase+1.9.3.1
65520beta-galactosidase+3.2.1.23

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65520C14:01.2
    65520C16:04.9
    65520C17:01.5
    65520C18:01.8
    65520C18:1ω9c4.8
    65520C16:1ω7c alcohol1.2
    65520C15:0 iso2.7
    65520C16:0 iso6.7
    65520C17:0 iso4.3
    65520C18:0 iso1
    65520C15:0 anteiso26.4
    65520C17:0 anteiso32
    65520C17:1 iso I /C17:1 anteiso B0.4
    65520C18:1ω7c / C18:1ω6c5.5
  • type of FA analysis: whole cell analysis
  • incubation medium: modified LB
  • incubation temperature: 37
  • library/peak naming table: TSBA 6.0
  • system: MIS MIDI
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
65520sediment of saline lake2015-08Yuncheng Saline Lake, Yuncheng, Shanxi ProvinceChinaCHNAsia34.8075110.69modified LB10 g/l casein tryptone, 5 g/l yeast extract, 200 g/l NaCl5 days30
67771From salt lakeYuncheng Saline Lake, ShanxiChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Sediment
#Condition#Saline

Sequence information

16S sequences

  • @ref: 65520
  • description: 16S rRNA gene sequence
  • accession: KX818201
  • database: nuccore

GC content

@refGC-contentmethod
6552052.1high performance liquid chromatography (HPLC)
6777152.1

External links

@ref: 65520

culture collection no.: KCTC 33792, MCCC 1K00567

literature

  • topic: Phylogeny
  • Pubmed-ID: 29148364
  • title: Salibacterium lacus sp. nov., a halophilic, non-spore-forming bacterium isolated from sediment of a saline lake.
  • authors: Wang YQ, Liu XM, Tang J, Yang GQ, Yu Z
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002467
  • year: 2017
  • mesh: Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Lakes/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65520Yue-qiang Wang, Xiao-ming Liu, Jia Tang, Gui-qin Yang and Zhen YuSalibacterium lacus sp. nov., a halophilic, non-spore-forming bacterium isolated from sediment of a saline lake10.1099/ijsem.0.002467IJSEM 68: 113-117 201829148364
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc