Strain identifier

BacDive ID: 158619

Type strain: Yes

Species: Nannocystis konarekensis

Strain Designation: MNa11734

NCBI tax ID(s): 2066023 (species)

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General

@ref: 65343

BacDive-ID: 158619

DSM-Number: 104509

keywords: 16S sequence, Bacteria, spore-forming, mesophilic, Gram-negative, motile, rod-shaped

description: Nannocystis konarekensis MNa11734 is a spore-forming, mesophilic, Gram-negative bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 2066023
  • Matching level: species

doi: 10.13145/bacdive158619.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Myxococcales
  • family: Nannocystaceae
  • genus: Nannocystis
  • species: Nannocystis konarekensis
  • full scientific name: Nannocystis konarekensis Mohr et al. 2018

@ref: 65343

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Myxococcales

family: Nannocystaceae

genus: Nannocystis

species: Nannocystis konarekensis

strain designation: MNa11734

type strain: yes

Morphology

cell morphology

  • @ref: 65343
  • gram stain: negative
  • cell length: 2-3.25 µm
  • cell width: 1.1-1.5 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: gliding

colony morphology

@refcolony colormedium used
65343orangeP agar
65343transparentVy/2 agar

Culture and growth conditions

culture medium

@refnamegrowth
65343P agaryes
65343VY/2 AGARyes

culture temp

@refgrowthtypetemperaturerange
65343positivegrowth25-37mesophilic
65343positiveoptimum37mesophilic
65343nogrowth44thermophilic

culture pH

@refabilitytypepHPH range
65343positivegrowth6.5-10alkaliphile
65343positiveoptimum8-10

Physiology and metabolism

spore formation

@refspore descriptiontype of sporespore formation
65343Myxospores are 0.8-0.9 µm wide and 1.7-1.8 µm long.myxosporeyes
65343Orange-coloured scattered sporangioles can be observed on water agar with E. coli bait.sporeyes

observation

  • @ref: 65343
  • observation: No real fruiting body formation could be observed

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6534317698chloramphenicolyesyes50 µg/mL
6534317833gentamicinyesyes50 µg/mL
653436104kanamycinyesyes50 µg/mL
653438309polymyxin byesyes50 µg/mL
653439215spectinomycinyesyes50 µg/mL
6534328971ampicillinyesyes50 µg/mL
6534323066cephalosporinyesyes50 µg/mL
6534371321fusidateyesyes50 µg/mL
6534345924trimethoprimyesyes50 µg/mL
6534329693thiostreptonyesyes50 µg/mL
6534328669bacitracinyesyes50 µg/mL
6534327701oxytetracyclineyesyes50 µg/mL

enzymes

@refvalueactivityec
65343catalase+1.11.1.6
65343cytochrome oxidase+1.9.3.1
65343alkaline phosphatase+3.1.3.1
65343leucine arylamidase+3.4.11.1
65343valine arylamidase+
65343acid phosphatase+3.1.3.2
65343naphthol-AS-BI-phosphohydrolase+
65343esterase (C 4)+
65343esterase Lipase (C 8)+
65343lipase (C 14)+
65343cystine arylamidase+3.4.11.3
65343trypsin+3.4.21.4
65343chymotrypsin+3.4.4.5
65343alpha-galactosidase-3.2.1.22
65343beta-galactosidase-3.2.1.23
65343beta-glucuronidase-3.2.1.31
65343alpha-glucosidase-3.2.1.20
65343beta-glucosidase-3.2.1.21
65343N-acetyl-beta-glucosaminidase-3.2.1.52
65343alpha-mannosidase-3.2.1.24
65343alpha-fucosidase-3.2.1.51

Isolation, sampling and environmental information

isolation

  • @ref: 65343
  • sample type: soil
  • geographic location: Konarak County, Sistan and Baluchestan Province. Konarak is situated on the western coast of Chabahar Bay, on the Makran coast, Gulf of Oman
  • country: Iran
  • origin.country: IRN
  • continent: Asia
  • latitude: 25.3602
  • longitude: 60.3994
  • enrichment culture: myxovirescin medium
  • enrichment culture composition: (1.0 % casein peptone, 0.005 % CaCl2, 0.025 % MgSO4, 1 mg l-1 CoCl2, 100 mM HEPES, pH 7.0) with 10 % A medium (0.8 % starch, 0.4 % soy flour, 0.2 % yeast, 0.1 % CaCl2, 0.1 % MgSO4, 100 mM Hepes, 1 ml l-1 Fe-EDTA, 4 ml l-1 glycerin, pH 7.4) and vitamin B 12 (final concentration, 500 µg l-1)
  • enrichment culture duration: 7 days
  • enrichment culture temperature: 30
  • isolation procedure: incubated on a rotary shaker at 160 r.p.m

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_86715.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_2095;96_15187;97_18549;98_59990;99_86715&stattab=map
  • Last taxonomy: Nannocystis konarekensis subclade
  • 16S sequence: KY381122
  • Sequence Identity:
  • Total samples: 435
  • soil counts: 148
  • aquatic counts: 103
  • animal counts: 156
  • plant counts: 28

Sequence information

16S sequences

  • @ref: 65343
  • description: 16S rRNA gene sequence
  • accession: KY381122
  • database: nuccore

GC content

@refGC-contentmethod
6534373.3genome sequence analysis
6534372.1high performance liquid chromatography (HPLC)

External links

@ref: 65343

culture collection no.: DSM 104509, NCCB 100618

literature

  • topic: Phylogeny
  • Pubmed-ID: 29458458
  • title: Nannocystis konarekensis sp. nov., a novel myxobacterium from an Iranian desert.
  • authors: Mohr KI, Moradi A, Glaeser SP, Kampfer P, Gemperlein K, Nubel U, Schumann P, Muller R, Wink J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002569
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Desert Climate, Fatty Acids/chemistry, Iran, Myxococcales/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65343Kathrin I. Mohr, Azam Moradi, Stefanie P. Glaeser, Peter Kämpfer, Katja Gemperlein, Ulrich Nübel, Peter Schumann, Rolf Müller, Joachim WinkNannocystis konarekensis sp. nov., a novel myxobacterium from an Iranian desert10.1099/ijsem.0.002569IJSEM 68: 721-729 201829458458
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/