Strain identifier

BacDive ID: 158587

Type strain: Yes

Species: Tenacibaculum insulae

Strain Designation: JDTF-31

Strain history: <- Jung-Hoon YOON, Sungkyunkwan Univ.

NCBI tax ID(s): 2029677 (species)

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General

@ref: 65301

BacDive-ID: 158587

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Tenacibaculum insulae JDTF-31 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from tidal flat sediment sample.

NCBI tax id

  • NCBI tax id: 2029677
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Jung-Hoon YOON, Sungkyunkwan Univ.

doi: 10.13145/bacdive158587.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Tenacibaculum
  • species: Tenacibaculum insulae
  • full scientific name: Tenacibaculum insulae Park et al. 2018

@ref: 65301

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Tenacibaculum

species: Tenacibaculum insulae

strain designation: JDTF-31

type strain: yes

Morphology

cell morphology

  • @ref: 65301
  • gram stain: negative
  • cell length: 0.8-10 µm
  • cell width: 0.2-0.4 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 65301
  • colony size: 0.5-1 mm
  • colony color: greyish yellow
  • colony shape: circular
  • incubation period: 3 days
  • medium used: marine agar 2216

pigmentation

  • @ref: 65301
  • production: no
  • name: flexirubin-type pigments

Culture and growth conditions

culture medium

  • @ref: 65301
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
65301positiveoptimum25mesophilic
65301positivegrowth10psychrophilic
65301positivegrowth28mesophilic
65301nogrowth4psychrophilic
65301nogrowth30mesophilic
67771positivegrowth25mesophilic

culture pH

@refabilitytypepH
65301positiveoptimum7-8
65301positivegrowth6
65301nogrowth5.5

Physiology and metabolism

oxygen tolerance

  • @ref: 67771
  • oxygen tolerance: aerobe

spore formation

  • @ref: 65301
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
65301NaClpositivegrowth1.5-4 %(w/v)
65301NaClpositiveoptimum2 %(w/v)

observation

  • @ref: 65301
  • observation: Mg2+ ions are not required for growth

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
65301casein+hydrolysis
65301cellobiose-builds acid from17057
65301D-fructose-builds acid from15824
65301D-galactose-builds acid from12936
65301D-glucose-builds acid from17634
65301D-mannitol-builds acid from16899
65301D-mannose-builds acid from16024
65301D-ribose-builds acid from16988
65301D-sorbitol-builds acid from17924
65301D-xylose-builds acid from65327
65301esculin-hydrolysis4853
65301gelatin-hydrolysis5291
65301hypoxanthine-hydrolysis17368
65301L-arabinose-builds acid from30849
65301L-rhamnose-builds acid from62345
65301L-tyrosine+hydrolysis17895
65301lactose-builds acid from17716
65301maltose-builds acid from17306
65301melezitose-builds acid from6731
65301melibiose-builds acid from28053
65301myo-inositol-builds acid from17268
65301nitrate+reduction17632
65301raffinose-builds acid from16634
65301starch-hydrolysis28017
65301sucrose-builds acid from17992
65301trehalose-builds acid from27082
65301tween 80+hydrolysis53426
65301urea-hydrolysis16199
65301xanthine-hydrolysis15318

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
653016472lincomycinyesyes15 µg (disc)
6530128368novobiocinyesyes15 µg (disc)
6530116869oleandomycinyesyes15 µg (disc)
653013542cephalothinyesyes30 µg (disc)
6530127902tetracyclineyesyes30 µg (disc)
653013393carbenicillinyesyes100 µg (disc)
6530117698chloramphenicolyesyes100 µg (disc)
6530128971ampicillinyesyes10 µg (disc)
6530118208penicillin gyesyes20 Unit (disc)
6530117833gentamicinyesyes30 µg (disc)
653016104kanamycinyesyes30 µg (disc)
653017507neomycinyesyes30 µg (disc)
6530117076streptomycinyesyes50 µg (disc)
653018309polymyxin byesyes100 Unit (disc)

metabolite production

  • @ref: 65301
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
65301catalase+1.11.1.6
65301cytochrome oxidase+1.9.3.1
65301alkaline phosphatase+3.1.3.1
65301leucine arylamidase+3.4.11.1
65301valine arylamidase+
65301acid phosphatase+3.1.3.2
65301naphthol-AS-BI-phosphohydrolase+
65301N-acetyl-beta-glucosaminidase+3.2.1.52
65301esterase (C 4)-
65301esterase Lipase (C 8)-
65301lipase (C 14)-
65301cystine arylamidase-3.4.11.3
65301trypsin-3.4.21.4
65301alpha-chymotrypsin-3.4.21.1
65301alpha-galactosidase-3.2.1.22
65301beta-galactosidase-3.2.1.23
65301alpha-glucosidase-3.2.1.20
65301beta-glucuronidase-3.2.1.31
65301alpha-mannosidase-3.2.1.24
65301alpha-fucosidase-3.2.1.51
65301beta-glucosidase-3.2.1.21

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture temperature
65301tidal flat sediment sampleJindo, an island of South Korea, on the Yellow SeaRepublic of KoreaKORAsiamarine agar 221625
67771From mud flatJindo-gunRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Tidal flat
#Environmental#Aquatic#Marine

Sequence information

16S sequences

  • @ref: 65301
  • description: 16S rRNA gene sequence
  • accession: MF765760
  • database: nuccore

GC content

  • @ref: 65301
  • GC-content: 31.3
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 65301

culture collection no.: KCTC 52749, NBRC 112783

literature

  • topic: Phylogeny
  • Pubmed-ID: 29148365
  • title: Tenacibaculum insulae sp. nov., isolated from a tidal flat.
  • authors: Park S, Choi J, Choi SJ, Yoon JH
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002487
  • year: 2017
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Islands, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Seawater/*microbiology, Sequence Analysis, DNA, Tenacibaculum/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65301Sooyeon Park, Jeehyun Choi, Su Jung Choi, Jung-Hoon YoonTenacibaculum insulae sp. nov., isolated from a tidal flat10.1099/ijsem.0.002487IJSEM 68: 228-333 201829148365
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc