Strain identifier

BacDive ID: 158473

Type strain: Yes

Species: Streptomyces inhibens

Strain Designation: NEAU-D10

Strain history: <- J. Zhao, School of Life Science, Northeast Agricultural Univ., Harbin, P.R. China; NEAU-D10 <- L. Jin, School of Life Science, Northeast Agricultural Univ., Harbin, P.R. China; NEAU-D10

NCBI tax ID(s): 2293571 (species)

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General

@ref: 65149

BacDive-ID: 158473

DSM-Number: 106197

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Streptomyces inhibens NEAU-D10 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 2293571
  • Matching level: species

strain history

  • @ref: 65149
  • history: <- J. Zhao, School of Life Science, Northeast Agricultural Univ., Harbin, P.R. China; NEAU-D10 <- L. Jin, School of Life Science, Northeast Agricultural Univ., Harbin, P.R. China; NEAU-D10

doi: 10.13145/bacdive158473.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces inhibens
  • full scientific name: Streptomyces inhibens Jin et al. 2019

@ref: 65149

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces inhibens

full scientific name: Streptomyces inhibens Jin et al. 2019

strain designation: NEAU-D10

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
67813positive
69480no95.241
69480positive100

colony morphology

@refcolony colormedium used
69311Beige (1001)suter without tyrosine
69311Beige (1001)suter with tyrosine
69311Mouse grey (7005)ISP 4
69311Ochre yellow (1024), black grey (7021)ISP 5
69311Ochre yellow (1024), black grey (7021)ISP 7
69311Sand yellow (1002)ISP 6
69311Signal black (9004)ISP 2
69311Signal black (9004), sepia brown (8014)ISP 3

multicellular morphology

@refforms multicellular complexfurther descriptioncomplex namecomplex colormedium name
67813yesforms well-developed, branched substrate hyphae and aerial mycelium that differentiate into spiral spore chains with oval spores (1.0-1.4 x 0.8-0.9 µm).
69311yesAerial myceliumMoss grey (7003)ISP 2
69311yesAerial myceliumMoss grey (7003), signal white (9003)ISP 3
69311yesAerial myceliumTelegrey 4 (7047)ISP 4
69311yesAerial myceliumSignal white (9003), silk grey (7044), moss grey (7003)ISP 5
69311noAerial myceliumISP 6
69311yesAerial myceliumSignal white (9003), silk grey (7044), moss grey (7003)ISP 7
69311yesAerial myceliumSignal white (9003)suter with tyrosine
69311yesAerial myceliumSignal white (9003)suter without tyrosine

pigmentation

@refproductionname
69311noMelanin
69311nosoluble pigment

multimedia

  • @ref: 65149
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_106197.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

  • @ref: 65149
  • name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/65
  • composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
65149positivegrowth28mesophilic
67813positivegrowth15-37
67813positiveoptimum28mesophilic

culture pH

@refabilitytypepHPH range
67813positivegrowth5-9alkaliphile
67813positiveoptimum8

Physiology and metabolism

oxygen tolerance

  • @ref: 67813
  • oxygen tolerance: aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
67813spiral spore chains with oval spores (1.0-1.4 x 0.8-0.9 µm). The spore surface is wrinkled.sporeyes
69480yes100
69481yes100

halophily

  • @ref: 67813
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-8 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6931122599arabinose-growth
6781317632nitrate-reduction
6781362968cellulose-assimilation
6781328017starch+hydrolysis
678135291gelatin+hydrolysis
67813milk+assimilation
6781353424tween 20-hydrolysis
6781353423tween 40-hydrolysis
6781353426tween 80-hydrolysis
678134853esculin-hydrolysis
6781365327D-xylose+builds acid from
6781316988D-ribose+builds acid from
6781317634D-glucose+builds acid from
6781317306maltose+builds acid from
6781312936D-galactose+builds acid from
6781315824D-fructose+builds acid from
6781316024D-mannose+builds acid from
6781317716lactose+builds acid from
6781316899D-mannitol+builds acid from
6781362345L-rhamnose+builds acid from
6781317924D-sorbitol+builds acid from
6781317268myo-inositol+builds acid from
6781316634raffinose+builds acid from
6781330849L-arabinose-builds acid from
6781317992sucrose-builds acid from
6781316977L-alanine+nitrogen source
6781317196L-asparagine+nitrogen source
6781329991L-aspartate+nitrogen source
6781315428glycine+nitrogen source
6781317203L-proline+nitrogen source
6781317115L-serine+nitrogen source
6781316857L-threonine+nitrogen source
6781317895L-tyrosine+nitrogen source
6781329016arginine-nitrogen source
6781316919creatine-nitrogen source
6781329988L-glutamate-nitrogen source
6781318050L-glutamine-nitrogen source
6931162968cellulose-growth
6931128757fructose+growth
6931117234glucose+growth
6931117268inositol+growth
6931137684mannose+growth
6931116634raffinose+growth
6931126546rhamnose-growth
6931117992sucrose-growth
6931118222xylose+growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 67813
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
67813urease+3.5.1.5
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382leucine arylamidase+3.4.11.1
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase+3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69311---+-+-+--+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69311++/-+/-+/-++/-+/-++++++-+/-+/-+++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
65149soilNortheast Agricultural University, Harbin City, Heilongjiang Province (45° 44' 26.2'' N, 126° 43' 14.9'' E)ChinaCHNAsia45.7406126.721
67813rhizosphere soil of wheatNortheast Agriculture University, Harbin, HeilongjiangChinaCHNAsia45.75126.733humic acid-vitamin agarsupplemented with cycloheximide (50 mg l-1) and nalidixic acid (20 mg l-1)21 days28sample was ground into powder and then suspended in sterile distilled water followed by a standard serial dilution technique

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Sequence information

16S sequences

  • @ref: 65149
  • description: Streptomyces sp. NEAU-D10 16S ribosomal RNA gene, partial sequence
  • accession: MH727580
  • length: 1519
  • database: ena
  • NCBI tax ID: 2293571

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces inhibens NEAU-D102862256214draftimg2293571
67813Streptomyces inhibens NEAU-D10GCA_003389455scaffoldncbi2293571

GC content

@refGC-contentmethod
6514970.5
6781370.5genome sequence analysis

Genome-based predictions

predictions

  • trait: spore-forming
  • prediction: yes
  • confidence: 100
  • training_data: no

External links

@ref: 65149

culture collection no.: DSM 106197, CGMCC 4.7469

straininfo link

  • @ref: 111270
  • straininfo: 401007

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny30605073Streptomyces inhibens sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.).Jin L, Zhao Y, Song W, Duan L, Jiang S, Wang X, Zhao J, Xiang WInt J Syst Evol Microbiol10.1099/ijsem.0.0032042019Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/isolation & purification, Triticum/*microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny33783591Streptomyces liangshanensis sp. nov., a novel actinomycete isolated from rhizosphere soil of Fagopyrum tataricum.Guo YH, Tang XK, Hu SR, Zhou ML, Gao JArch Microbiol10.1007/s00203-021-02294-72021*Actinobacteria/genetics, Bacterial Typing Techniques, DNA, Bacterial/genetics, *Fagopyrum/microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Species Specificity, *Streptomyces/classification/geneticsTranscriptome

Reference

@idauthorstitledoi/urlcataloguejournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65149Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-106197Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106197)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67813Liying Jin, Yang Zhao, Wei Song, Liping Duan, Shanwen Jiang, Xiangjing Wang, Junwei Zhao, Wensheng XiangStreptomyces inhibens sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.)10.1099/ijsem.0.003204IJSEM 69: 688-695 2019
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69311Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%20106197.pdf
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
111270Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401007.1