Strain identifier

BacDive ID: 158411

Type strain: Yes

Species: Apilactobacillus quenuiae

Strain Designation: HV_6

Strain history: DSM 104127 <-- J. A. Rothman, Univ. of California, Riverside, USA; HV_6.

NCBI tax ID(s): 2008377 (species)

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General

@ref: 65087

BacDive-ID: 158411

DSM-Number: 104127

keywords: rod-shaped, Gram-positive, aerobe, Bacteria, 16S sequence, genome sequence, colony-forming

description: Apilactobacillus quenuiae HV_6 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from Adult bee gut, Augochlorella pomoniella.

NCBI tax id

  • NCBI tax id: 2008377
  • Matching level: species

strain history

doi: 10.13145/bacdive158411.20211221.6

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/firmicutes
  • domain: Bacteria
  • phylum: Firmicutes
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Apilactobacillus
  • species: Apilactobacillus quenuiae
  • full scientific name: Apilactobacillus quenuiae (McFrederick et al. 2018) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus quenuiae

@ref: 65087

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Apilactobacillus

species: Apilactobacillus quenuiae

full scientific name: Apilactobacillus quenuiae (McFrederick et al. 2018) Zheng et al. 2020

strain designation: HV_6

type strain: yes

Morphology

cell morphology

  • @ref: 65392
  • gram stain: positive
  • cell length: 0.9-2.3 µm
  • cell width: 0.3-0.4 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 65392
  • colony color: white
  • colony shape: circular
  • medium used: De Man, Rogosa, Sharpe agar

Culture and growth conditions

culture medium

  • @ref: 65087
  • name: MRS MEDIUM (DSMZ Medium 11)
  • growth: yes
  • link: https://bacmedia.dsmz.de/medium/11
  • composition: Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
65392positivegrowth15-35
65392positiveoptimum30-35mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepH
65392positivegrowth2-7
65392positiveoptimum5-6

Physiology and metabolism

oxygen tolerance

  • @ref: 65392
  • oxygen tolerance: aerobe

spore formation

  • @ref: 65392
  • spore formation: no

observation

  • @ref: 65392
  • observation: heterofermentive

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6539215824D-fructose+builds acid from
6539217634D-glucose+builds acid from
6539217234glucose+fermentation
6539217992sucrose+builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6539217076streptomycinyesyes10 µg (disc)
6539228669bacitracinyesyes10 Unit (disc)
6539217334penicillinyesyes10 Unit (disc)
6539248923erythromycinyesyes15 µg (disc)
65392209807cefoxitinyesyes30 µg (disc)
6539227902tetracyclineyesyes30 µg (disc)
653923393carbenicillinyesyes100 µg (disc)
653928309polymyxin byesyes300 Unit (disc)
6539228971ampicillinyesyes10 µg (disc)
6539217698chloramphenicolyesyes30 µg (disc)
6539228001vancomycinyesyes30 µg (disc)
653926104kanamycinyesyes30 µg (disc)

enzymes

@refvalueactivityec
65392alkaline phosphatase+3.1.3.1
65392leucine arylamidase+3.4.11.1
65392acid phosphatase+3.1.3.2
65392naphthol-AS-BI-phosphohydrolase+
65392esterase (C 4)+
65392catalase+1.11.1.6

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedurehost species
65087Adult bee gut, Augochlorella pomoniella33.9706N, 117.3201WUSAUSANorth America
65392gut of a sweat bee (Augochlorella pomoniella)campus of UC Riverside, Riverside, CAUSAUSANorth AmericaDe Man, Rogosa, Sharpe agarsupplemented with 20 % D-fructose2-3 days24Bee guts were dissected in sterile water then homogenized in sterile physiologic saline. The gut homogenates were plated on De Man, Rogosa, Sharpe agar plates supplemented with 20 % D-fructose, then incubated in an aerobic environment for 2-3 days at 24°C.
67770Gut of a sweat bee (Halictus ligatus) caught at the Hornsby Bend Center for Environmental ResearchAustin, TXUSAUSANorth AmericaHalictus ligatus

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Insecta
#Host Body-Site#Gastrointestinal tract

Sequence information

16S sequences

  • @ref: 65087
  • description: Lactobacillus quenuiae strain HV_6 16S ribosomal RNA gene, partial sequence
  • accession: KX656667
  • length: 1422
  • database: ena
  • NCBI tax ID: 2008377

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Apilactobacillus quenuiae HV_6GCA_002994005contigncbi2008377
67770Apilactobacillus quenuiae strain HV_6, whole genome shotgun sequencing projectPOSN00000000ncbi2008377

GC content

@refGC-contentmethod
6508730.3
6539230.3genome sequence analysis

External links

@ref: 65087

culture collection no.: DSM 104127, NRRL B-65474, JCM 33324

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215D.Gleim, M.Kracht, N.Weiss et. al.Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes sincehttp://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.html
65087Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-104127Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 104127)
65392Quinn S. McFrederick, Hoang Q. Vuong, Jason A. RothmanLactobacillus micheneri sp. nov., Lactobacillus timberlakei sp. nov. and Lactobacillus quenuiae sp. nov., lactic acid bacteria isolated from wild bees and flowers10.1099/ijsem.0.002758IJSEM 68: 1879-1884 201829648528
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/