Strain identifier
BacDive ID: 158411
Type strain:
Species: Apilactobacillus quenuiae
Strain Designation: HV_6
Strain history: DSM 104127 <-- J. A. Rothman, Univ. of California, Riverside, USA; HV_6.
NCBI tax ID(s): 2008377 (species)
General
@ref: 65087
BacDive-ID: 158411
DSM-Number: 104127
keywords: rod-shaped, Gram-positive, aerobe, Bacteria, 16S sequence, genome sequence, colony-forming
description: Apilactobacillus quenuiae HV_6 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from Adult bee gut, Augochlorella pomoniella.
NCBI tax id
- NCBI tax id: 2008377
- Matching level: species
strain history
doi: 10.13145/bacdive158411.20211221.6
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/firmicutes
- domain: Bacteria
- phylum: Firmicutes
- class: Bacilli
- order: Lactobacillales
- family: Lactobacillaceae
- genus: Apilactobacillus
- species: Apilactobacillus quenuiae
- full scientific name: Apilactobacillus quenuiae (McFrederick et al. 2018) Zheng et al. 2020
synonyms
- @ref: 20215
- synonym: Lactobacillus quenuiae
@ref: 65087
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Lactobacillaceae
genus: Apilactobacillus
species: Apilactobacillus quenuiae
full scientific name: Apilactobacillus quenuiae (McFrederick et al. 2018) Zheng et al. 2020
strain designation: HV_6
type strain: yes
Morphology
cell morphology
- @ref: 65392
- gram stain: positive
- cell length: 0.9-2.3 µm
- cell width: 0.3-0.4 µm
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 65392
- colony color: white
- colony shape: circular
- medium used: De Man, Rogosa, Sharpe agar
Culture and growth conditions
culture medium
- @ref: 65087
- name: MRS MEDIUM (DSMZ Medium 11)
- growth: yes
- link: https://bacmedia.dsmz.de/medium/11
- composition: Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
65392 | positive | growth | 15-35 | |
65392 | positive | optimum | 30-35 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
65392 | positive | growth | 2-7 |
65392 | positive | optimum | 5-6 |
Physiology and metabolism
oxygen tolerance
- @ref: 65392
- oxygen tolerance: aerobe
spore formation
- @ref: 65392
- spore formation: no
observation
- @ref: 65392
- observation: heterofermentive
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
65392 | 15824 | D-fructose | + | builds acid from |
65392 | 17634 | D-glucose | + | builds acid from |
65392 | 17234 | glucose | + | fermentation |
65392 | 17992 | sucrose | + | builds acid from |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is sensitive | sensitivity conc. | is resistant | resistance conc. |
---|---|---|---|---|---|---|---|
65392 | 17076 | streptomycin | yes | yes | 10 µg (disc) | ||
65392 | 28669 | bacitracin | yes | yes | 10 Unit (disc) | ||
65392 | 17334 | penicillin | yes | yes | 10 Unit (disc) | ||
65392 | 48923 | erythromycin | yes | yes | 15 µg (disc) | ||
65392 | 209807 | cefoxitin | yes | yes | 30 µg (disc) | ||
65392 | 27902 | tetracycline | yes | yes | 30 µg (disc) | ||
65392 | 3393 | carbenicillin | yes | yes | 100 µg (disc) | ||
65392 | 8309 | polymyxin b | yes | yes | 300 Unit (disc) | ||
65392 | 28971 | ampicillin | yes | yes | 10 µg (disc) | ||
65392 | 17698 | chloramphenicol | yes | yes | 30 µg (disc) | ||
65392 | 28001 | vancomycin | yes | yes | 30 µg (disc) | ||
65392 | 6104 | kanamycin | yes | yes | 30 µg (disc) |
enzymes
@ref | value | activity | ec |
---|---|---|---|
65392 | alkaline phosphatase | + | 3.1.3.1 |
65392 | leucine arylamidase | + | 3.4.11.1 |
65392 | acid phosphatase | + | 3.1.3.2 |
65392 | naphthol-AS-BI-phosphohydrolase | + | |
65392 | esterase (C 4) | + | |
65392 | catalase | + | 1.11.1.6 |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | enrichment culture | enrichment culture composition | enrichment culture duration | enrichment culture temperature | isolation procedure | host species |
---|---|---|---|---|---|---|---|---|---|---|---|
65087 | Adult bee gut, Augochlorella pomoniella | 33.9706N, 117.3201W | USA | USA | North America | ||||||
65392 | gut of a sweat bee (Augochlorella pomoniella) | campus of UC Riverside, Riverside, CA | USA | USA | North America | De Man, Rogosa, Sharpe agar | supplemented with 20 % D-fructose | 2-3 days | 24 | Bee guts were dissected in sterile water then homogenized in sterile physiologic saline. The gut homogenates were plated on De Man, Rogosa, Sharpe agar plates supplemented with 20 % D-fructose, then incubated in an aerobic environment for 2-3 days at 24°C. | |
67770 | Gut of a sweat bee (Halictus ligatus) caught at the Hornsby Bend Center for Environmental Research | Austin, TX | USA | USA | North America | Halictus ligatus |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Arthropoda | #Insecta |
#Host Body-Site | #Gastrointestinal tract |
Sequence information
16S sequences
- @ref: 65087
- description: Lactobacillus quenuiae strain HV_6 16S ribosomal RNA gene, partial sequence
- accession: KX656667
- length: 1422
- database: ena
- NCBI tax ID: 2008377
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Apilactobacillus quenuiae HV_6 | GCA_002994005 | contig | ncbi | 2008377 |
67770 | Apilactobacillus quenuiae strain HV_6, whole genome shotgun sequencing project | POSN00000000 | ncbi | 2008377 |
GC content
@ref | GC-content | method |
---|---|---|
65087 | 30.3 | |
65392 | 30.3 | genome sequence analysis |
External links
@ref: 65087
culture collection no.: DSM 104127, NRRL B-65474, JCM 33324
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | D.Gleim, M.Kracht, N.Weiss et. al. | Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since | http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.html | |||
65087 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-104127 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 104127) | |||
65392 | Quinn S. McFrederick, Hoang Q. Vuong, Jason A. Rothman | Lactobacillus micheneri sp. nov., Lactobacillus timberlakei sp. nov. and Lactobacillus quenuiae sp. nov., lactic acid bacteria isolated from wild bees and flowers | 10.1099/ijsem.0.002758 | IJSEM 68: 1879-1884 2018 | 29648528 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ |