Strain identifier

BacDive ID: 158405

Type strain: Yes

Species: Polynucleobacter campilacus

Strain Designation: MWH-Feld-100

Strain history: <- M. W. Hahn, Austrian Academy of Sci., Inst. Limnology; MWH-Feld-100

NCBI tax ID(s): 1743163 (species)

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General

@ref: 65081

BacDive-ID: 158405

DSM-Number: 24007

keywords: genome sequence, 16S sequence, Bacteria, aerobe, chemoorganoheterotroph, psychrophilic, motile, rod-shaped, colony-forming

description: Polynucleobacter campilacus MWH-Feld-100 is an aerobe, chemoorganoheterotroph, psychrophilic bacterium that forms circular colonies and was isolated from freshwater.

NCBI tax id

  • NCBI tax id: 1743163
  • Matching level: species

strain history

  • @ref: 65081
  • history: <- M. W. Hahn, Austrian Academy of Sci., Inst. Limnology; MWH-Feld-100

doi: 10.13145/bacdive158405.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Polynucleobacter
  • species: Polynucleobacter campilacus
  • full scientific name: Polynucleobacter campilacus Hahn et al. 2018

@ref: 65081

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Polynucleobacter

species: Polynucleobacter campilacus

full scientific name: Polynucleobacter campilacus Hahn et al. 2018

strain designation: MWH-Feld-100

type strain: yes

Morphology

cell morphology

  • @ref: 66620
  • cell length: 0.5-2.4 µm
  • cell width: 0.3-0.7 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 66620
  • colony color: non-pigmented
  • colony shape: circular
  • medium used: nutrient broth soytone yeast extract agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65081R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
66620nutrient broth soytone yeast extract agaryes

culture temp

@refgrowthtypetemperaturerange
65081positivegrowth22psychrophilic
66620positivegrowth5-28

Physiology and metabolism

oxygen tolerance

  • @ref: 66620
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 66620
  • type: chemoorganoheterotroph

halophily

@refsaltgrowthtested relationconcentration
66620NaClpositivegrowth0-0.4 %(w/v)
66620NaClnogrowth0.5-0.7 %(w/v)
66620NaClnogrowth>0.7 %(w/v)

observation

  • @ref: 66620
  • observation: Contains free-living Polynucleobacter strains dwelling in the water body of circum-neutral and probably also alkaline freshwater systems.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6662012936D-galactose-assimilation
6662015824D-fructose-assimilation
6662015971L-histidine-assimilation
6662016977L-alanine-assimilation
6662017196L-asparagine-assimilation
6662017272propionate-assimilation
6662017561L-cysteine-assimilation
6662017634D-glucose-assimilation
6662018024D-galacturonic acid-assimilation
6662018287L-fucose-assimilation
6662025115malate-assimilation
6662029806fumarate-assimilation
6662029985L-glutamate-assimilation
6662029991L-aspartate-assimilation
6662030031succinate-assimilation
6662030623oxalate-assimilation
6662036655glyoxylate-assimilation
66620391504-oxopentanoate-assimilation
6662053258citric acid-assimilation
6662062318D-lyxose-assimilation
6662015361pyruvate+assimilation
6662015603L-leucine+assimilation
6662015792malonate+assimilation
6662016024D-mannose+assimilation
6662016411acetic acid+assimilation
6662016452oxaloacetate+assimilation
6662017750betaine+assimilation
6662017924D-sorbitol+assimilation
6662029805glycolate+assimilation

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentsampling datecountryorigin.countrylatitudelongitudeenrichment cultureisolation procedure
65081freshwaterSouthern Black Forest, near Feldberg, Lake Feldsee (47.871° N, 8.033° E)Europe
66620watersample from the water columns of freshwater systemsLake Feldsee, Black forestEurope2006-12-30GermanyDEU47.8718.033nutrient broth soytone yeast extractfiltration acclimation method

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Freshwater

taxonmaps

  • @ref: 69479
  • File name: preview.99_93.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_12;96_74;97_77;98_84;99_93&stattab=map
  • Last taxonomy: Polynucleobacter
  • 16S sequence: MG952228
  • Sequence Identity:
  • Total samples: 42849
  • soil counts: 1815
  • aquatic counts: 34169
  • animal counts: 6310
  • plant counts: 555

Sequence information

16S sequences

  • @ref: 66620
  • description: Polynucleobacter sp. strain MWH-Feld-100 16S ribosomal RNA gene, partial sequence
  • accession: MG952228
  • length: 1367
  • database: ena
  • NCBI tax ID: 1743163

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Polynucleobacter campilacus MWH-Feld-100GCA_002206625contigncbi1743163
66792Polynucleobacter sp. MWH-Feld-100 strain MWH-Feld-1001743163.3wgspatric1743163
66792Polynucleobacter campilacus MWH-Feld-1002590828843completeimg1743163

GC content

  • @ref: 66620
  • GC-content: 45.2
  • method: genome sequence analysis

External links

@ref: 65081

culture collection no.: DSM 24007, LMG 29705

straininfo link

  • @ref: 111208
  • straininfo: 403699

literature

  • topic: Phylogeny
  • Pubmed-ID: 29939120
  • title: Polynucleobacter hirudinilacicola sp. nov. and Polynucleobacter campilacus sp. nov., both isolated from freshwater systems.
  • authors: Hahn MW, Koll U, Schmidt J, Huymann LR, Karbon G, Lang E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002880
  • year: 2018
  • mesh: Austria, Bacterial Typing Techniques, Base Composition, Burkholderiaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fresh Water/*microbiology, Germany, Lakes/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65081Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-24007Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24007)
66620Martin W. Hahn, Ulrike Koll, Johanna Schmidt, Lesley R. Huymann, Gerlinde Karbon, Elke LangPolynucleobacter hirudinilacicola sp. nov. and Polynucleobacter campilacus sp. nov., both isolated from freshwater systems10.1099/ijsem.0.002880IJSEM 68: 2593-2601 201829939120
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
111208Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403699.1