Strain identifier

BacDive ID: 158319

Type strain: Yes

Species: Glycomyces rhizosphaerae

Strain Designation: NEAU-C11

Strain history: <- J. Zhao; School of Life Science, Northeast Agricultural University, Harbin, P.R. CHINA; NEAU-C11<-W. Li

NCBI tax ID(s): 2054422 (species)

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General

@ref: 64995

BacDive-ID: 158319

DSM-Number: 104646

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Glycomyces rhizosphaerae NEAU-C11 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from rhizosphere soil of soybean root [ Glycine max Merr.].

NCBI tax id

  • NCBI tax id: 2054422
  • Matching level: species

strain history

  • @ref: 64995
  • history: <- J. Zhao; School of Life Science, Northeast Agricultural University, Harbin, P.R. CHINA; NEAU-C11<-W. Li

doi: 10.13145/bacdive158319.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Glycomycetales
  • family: Glycomycetaceae
  • genus: Glycomyces
  • species: Glycomyces rhizosphaerae
  • full scientific name: Glycomyces rhizosphaerae Li et al. 2018

@ref: 64995

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Glycomycetaceae

genus: Glycomyces

species: Glycomyces rhizosphaerae

full scientific name: Glycomyces rhizosphaerae Li et al. 2018

strain designation: NEAU-C11

type strain: yes

Morphology

cell morphology

  • @ref: 65300
  • gram stain: positive

multicellular morphology

  • @ref: 65300
  • forms multicellular complex: yes
  • further description: actinomycete which forms abundant substrate mycelia but no aerial mycelia

multimedia

  • @ref: 64995
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_104646.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
64995GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
64995STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
65300ISP2yes
65300Czapek's agaryes
65300Nutrient agar (NA)yes
65300modified Bennett's agaryes
65300ISP3yes
65300ISP4yes
65300ISP5yes
65300ISP6yes
65300ISP7yes

culture temp

@refgrowthtypetemperaturerange
64995positivegrowth28mesophilic
65300positivegrowth15-40
65300positiveoptimum28mesophilic

culture pH

@refabilitytypepHPH range
65300positivegrowth7-11alkaliphile
65300positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 65300
  • oxygen tolerance: aerobe

halophily

  • @ref: 65300
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %

observation

  • @ref: 65300
  • observation: No diffusible pigment is observed on any of the media tested.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6530062968cellulose+/-hydrolysis
6530016919creatine+/-assimilation
6530015824D-fructose+carbon source
6530017634D-glucose+carbon source
6530016899D-mannitol+carbon source
6530016634raffinose+carbon source
6530016988D-ribose-carbon source
6530017924D-sorbitol+carbon source
6530065327D-xylose-carbon source
653004853esculin+/-hydrolysis
6530028260galactose+/-assimilation
653005291gelatin+hydrolysis
6530015428glycine-nitrogen source
6530017268myo-inositol+carbon source
6530016977L-alanine-nitrogen source
6530030849L-arabinose+carbon source
6530017196L-asparagine+/-assimilation
6530029991L-aspartate+nitrogen source
6530029985L-glutamate+nitrogen source
6530017203L-proline+nitrogen source
6530062345L-rhamnose+carbon source
6530017115L-serine+nitrogen source
6530016857L-threonine+nitrogen source
6530017895L-tyrosine-nitrogen source
6530017716lactose+carbon source
6530017306maltose+carbon source
65300milk-assimilation
6530017632nitrate-reduction
6530028017starch+hydrolysis
6530017992sucrose+carbon source
6530053423tween 40+hydrolysis
6530053426tween 80+hydrolysis

metabolite production

  • @ref: 65300
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
65300urease-3.5.1.5
65300cellulase-3.2.1.4

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
64995rhizosphere soil of soybean root [ Glycine max (L.) Merr.]Heilongjiang province, Harbin (45° 45' N, 126° 41' E)ChinaCHNAsia45.75126.683
65300rhizosphere soil of wheat (Triticum aestivum L.)Langfang, Hebei ProvinceChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere
#Host Body-Site#Plant#Root (Rhizome)
#Environmental#Terrestrial#Soil

Safety information

risk assessment

  • @ref: 64995
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64995
  • description: Glycomyces rhizosphaerae strain NEAU-C11 16S ribosomal RNA gene, partial sequence
  • accession: KY744945
  • length: 1523
  • database: ena
  • NCBI tax ID: 2054422

GC content

@refGC-contentmethod
6499571.9thermal denaturation, midpoint method (Tm)
6530071.8-72thermal denaturation, midpoint method (Tm)

External links

@ref: 64995

culture collection no.: DSM 104646, CGMCC 4.7396

straininfo link

  • @ref: 111130
  • straininfo: 398954

literature

  • topic: Phylogeny
  • Pubmed-ID: 29134931
  • title: Glycomyces rhizosphaerae sp. nov., isolated from the root and rhizosphere soil of wheat (Triticum aestivum L.).
  • authors: Li W, Zhao J, Shi L, Wang J, Wang H, Wang X, Xiang W
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002488
  • year: 2017
  • mesh: Actinobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Nucleic Acid Hybridization, *Phylogeny, Plant Roots/microbiology, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Triticum/*microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64995Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-104646Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 104646)
65300Wenchao Li, Junwei Zhao, Linlin Shi, Jiabin Wang, Han Wang, Xiangjing Wang, Wensheng XiangGlycomyces rhizosphaerae sp. nov., isolated from the root and rhizosphere soil of wheat (Triticum aestivum L.)10.1099/ijsem.0.002488IJSEM 68: 223-227 201829134931
111130Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID398954.1