Strain identifier

BacDive ID: 158293

Type strain: Yes

Species: Paenibacillus arcticus

Strain Designation: MME2_R6

Strain history: M.-J. Seo; Incheon Natl. Univ., South Korea; MME2_R6.

NCBI tax ID(s): 1682150 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 8.1 (current version)

General

@ref: 64953

BacDive-ID: 158293

DSM-Number: 108811

keywords: 16S sequence, Bacteria, aerobe, spore-forming, psychrophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Paenibacillus arcticus MME2_R6 is an aerobe, spore-forming, psychrophilic bacterium that forms circular colonies and was isolated from artic soil.

NCBI tax id

  • NCBI tax id: 1682150
  • Matching level: species

strain history

@refhistory
64953<- KCTC, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeonbuk, South Korea; KCTC33985
67770M.-J. Seo; Incheon Natl. Univ., South Korea; MME2_R6.

doi: 10.13145/bacdive158293.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus arcticus
  • full scientific name: Paenibacillus arcticus Cha et al. 2017

@ref: 64953

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus arcticus

full scientific name: Paenibacillus arcticus Cha et al. 2017

strain designation: MME2_R6

type strain: yes

Morphology

cell morphology

  • @ref: 65278
  • gram stain: positive
  • cell length: 1.7-2.5 µm
  • cell width: 0.6-0.8 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: peritrichous

colony morphology

@refcolony sizecolony colorcolony shapeincubation periodmedium used
652781-1.5 mmcreamcircular14 daysR2A
652781-1.5 mmcreamcircular14 daystryptic soy agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
64953R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
65278Reasoner's 2A agar (R2A)yes
65278tryptic soy agaryes

culture temp

@refgrowthtypetemperaturerange
64953positivegrowth20psychrophilic
65278positivegrowth4-24psychrophilic
65278positiveoptimum20psychrophilic
67770positivegrowth20psychrophilic

culture pH

@refabilitytypepH
65278positivegrowth7-8
65278positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 65278
  • oxygen tolerance: aerobe

spore formation

  • @ref: 65278
  • spore description: ellipsoidal spore
  • spore formation: yes

halophily

  • @ref: 65278
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-0.5 %(w/v)

observation

@refobservation
65278Does not require sodium ions for growth.
67770quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6527827613amygdalin-builds acid from
6527818305arbutin-builds acid from
65278casein-hydrolysis
6527817057cellobiose-builds acid from
6527815963ribitol-builds acid from
6527817108D-arabinose-builds acid from
6527818333D-arabitol-builds acid from
6527815824D-fructose-builds acid from
6527828847D-fucose-builds acid from
6527812936D-galactose-builds acid from
6527817634D-glucose-builds acid from
6527862318D-lyxose-builds acid from
6527816899D-mannitol-builds acid from
6527816024D-mannose+builds acid from
6527816988D-ribose-builds acid from
6527817924D-sorbitol-builds acid from
6527816443D-tagatose-builds acid from
6527865327D-xylose-builds acid from
6527816813galactitol-builds acid from
6527817113erythritol-builds acid from
652784853esculin-hydrolysis
65278esculin ferric citrate-builds acid from
652785291gelatin-hydrolysis
6527828066gentiobiose+builds acid from
6527817234glucose-fermentation
6527817754glycerol-builds acid from
6527828087glycogen-builds acid from
6527817268myo-inositol-builds acid from
6527815443inulin-builds acid from
6527830849L-arabinose-builds acid from
6527818403L-arabitol-builds acid from
6527818287L-fucose-builds acid from
6527862345L-rhamnose-builds acid from
6527817266L-sorbose-builds acid from
6527817895L-tyrosine-hydrolysis
6527865328L-xylose-builds acid from
6527817716lactose-builds acid from
6527817306maltose-builds acid from
652786731melezitose-builds acid from
6527828053melibiose+builds acid from
65278320061methyl alpha-D-glucopyranoside-builds acid from
6527843943methyl alpha-D-mannoside-builds acid from
6527874863methyl beta-D-xylopyranoside-builds acid from
65278506227N-acetylglucosamine-builds acid from
6527817632nitrate-reduction
65278potassium 2-dehydro-D-gluconate-builds acid from
65278potassium 5-dehydro-D-gluconate-builds acid from
6527832032potassium gluconate-builds acid from
6527816634raffinose-builds acid from
6527817814salicin-builds acid from
6527828017starch-builds acid from
6527828017starch-hydrolysis
6527817992sucrose-builds acid from
6527827082trehalose-builds acid from
6527832528turanose-builds acid from
6527853424tween 20-hydrolysis
6527853423tween 40-hydrolysis
6527853426tween 80-hydrolysis
6527817151xylitol-builds acid from

metabolite production

  • @ref: 65278
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
65278catalase+1.11.1.6
65278cytochrome oxidase-1.9.3.1
65278beta-galactosidase+3.2.1.23
65278arginine dihydrolase-3.5.3.6
65278urease-3.5.1.5
65278esterase (C 4)-
65278alkaline phosphatase-3.1.3.1
65278lipase (C 14)-
65278leucine arylamidase-3.4.11.1
65278valine arylamidase-
65278cystine arylamidase-3.4.11.3
65278trypsin-3.4.21.4
65278alpha-chymotrypsin-3.4.21.1
65278alpha-galactosidase-3.2.1.22
65278beta-glucuronidase-3.2.1.31
65278alpha-glucosidase-3.2.1.20
65278beta-glucosidase-3.2.1.21
65278N-acetyl-beta-glucosaminidase-3.2.1.52
65278alpha-mannosidase-3.2.1.24
65278alpha-fucosidase-3.2.1.51
65278esterase Lipase (C 8)+
65278acid phosphatase+3.1.3.2
65278naphthol-AS-BI-phosphohydrolase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65278C14:01.1
    65278C16:08.2
    65278C14:0 iso2.5
    65278C15:0 iso6.7
    65278C16:0 iso4.7
    65278C17:0 iso1.4
    65278C13:0 anteiso1.3
    65278C15:0 anteiso67.5
    65278C17:0 anteiso1.5
    65278C16?:?1ω11c1
  • type of FA analysis: whole cell analysis
  • incubation medium: tryptic soy agar
  • agar/liquid: agar
  • incubation temperature: 20
  • incubation time: 14
  • software version: Sherlock 6.2
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling datelatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
64953artic soilMidtre Lovenbreen glacier foreland, SvalbardNorwayNOREurope
65278arctic soilMidtre Lov enbreen glacier foreland at SvalbardNorwayNOREurope2013-0678.902512.1095tryptic soy agarpH 7.21 month20sample was diluted with 0.85 % (w/v) saline and plated onto tryptic soy agar. The plates were incubated at 20°C for 1 month.
67770Arctic soil of Midtre Lovénbreen glacier foreland at SvalbardNorwayNOREurope

isolation source categories

Cat1Cat2Cat3
#Climate#Cold#Polar
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_30409.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_5165;97_6235;98_22626;99_30409&stattab=map
  • Last taxonomy: Paenibacillus arcticus subclade
  • 16S sequence: KP900012
  • Sequence Identity:
  • Total samples: 625
  • soil counts: 545
  • aquatic counts: 9
  • animal counts: 28
  • plant counts: 43

Safety information

risk assessment

  • @ref: 64953
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64953
  • description: Paenibacillus arcticus strain MME2_R6 16S ribosomal RNA gene, partial sequence
  • accession: KP900012
  • length: 1469
  • database: ena
  • NCBI tax ID: 1682150

GC content

  • @ref: 67770
  • GC-content: 44.2
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 64953

culture collection no.: DSM 108811, JCM 30981, KCTC 33985, PAMC 28731

straininfo link

  • @ref: 111110
  • straininfo: 406704

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28920828Paenibacillus arcticus sp. nov., isolated from Arctic soil.Cha IT, Cho ES, Yoo Y, Seok YJ, Park I, Lim HS, Park JM, Roh SW, Nam YD, Choi HJ, Lee YK, Seo MJInt J Syst Evol Microbiol10.1099/ijsem.0.0022992017Arctic Regions, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Paenibacillus/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny31073896Paenibacillus psychroresistens sp. nov., isolated from the soil of an Arctic glacial retreat.Cha IT, Cho ES, Lee YK, Roh SW, Seo MJJ Microbiol10.1007/s12275-019-8666-x2019Arctic Regions, Bacterial Typing Techniques, Ice Cover/*microbiology, Paenibacillus/*classification, Phylogeny, Soil, *Soil Microbiology

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64953Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-108811Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 108811)
65278In-Tae Cha, Eui-Sang Cho, Yesol Yoo, Yoon Ji Seok, Inhye Park, Hee Seon Lim, Jung-Min Park, Seong Woon Roh, Young-Do Nam, Hak-Jong Choi, Yoo Kyung Lee, Myung-Ji SeoPaenibacillus arcticus sp. nov., isolated from Arctic soil10.1099/ijsem.0.002299IJSEM 67: 4385-4389 201728920828
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
111110Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID406704.1