Strain identifier

BacDive ID: 158275

Type strain: Yes

Species: Phaeobacter piscinae

Strain Designation: 27-4

Strain history: <- E. Sonnenschein, Technical University of Denmark, Kgs. Lyngby, Denmark; 27-4 <- M. Hjelm and L. Gram

NCBI tax ID(s): 1580596 (species)

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General

@ref: 64935

BacDive-ID: 158275

DSM-Number: 103509

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Phaeobacter piscinae 27-4 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from moribund turbot larvae from rearing units.

NCBI tax id

  • NCBI tax id: 1580596
  • Matching level: species

strain history

  • @ref: 64935
  • history: <- E. Sonnenschein, Technical University of Denmark, Kgs. Lyngby, Denmark; 27-4 <- M. Hjelm and L. Gram

doi: 10.13145/bacdive158275.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Phaeobacter
  • species: Phaeobacter piscinae
  • full scientific name: Phaeobacter piscinae Sonnenschein et al. 2017

@ref: 64935

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Phaeobacter

species: Phaeobacter piscinae

full scientific name: Phaeobacter piscinae Sonnenschein et al. 2017

strain designation: 27-4

type strain: yes

Morphology

cell morphology

  • @ref: 65413
  • gram stain: negative
  • cell length: 1.7-2.4 µm
  • cell width: 0.7-1.1 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: polar

colony morphology

  • @ref: 65413
  • colony color: light brown to brown
  • colony shape: circular
  • incubation period: 3 days
  • medium used: marine agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
64935BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
65413Marine agar (MA)yes

culture temp

@refgrowthtypetemperaturerange
64935positivegrowth25mesophilic
65413positivegrowth10-35
65413nogrowth8psychrophilic
65413nogrowth37mesophilic
65413positiveoptimum31-33mesophilic

culture pH

@refabilitytypepHPH range
65413positivegrowth6.5-8.5alkaliphile
65413positiveoptimum7.0

Physiology and metabolism

oxygen tolerance

  • @ref: 65413
  • oxygen tolerance: aerobe

observation

@refobservation
65413copper-resistant MIC=2.0-3.5 mM
65413sole lipoquinone is Q10
65413polar lipid profile comprises phosphatidylglycerol, diphosphatidylglycerol, phosphatidyl-ethanolamine, phosphatidylcholine, an aminolipid, a phospholipid and an unidentified lipid

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6541317634D-glucose+assimilation
6541316024D-mannose+assimilation
6541317924D-sorbitol+assimilation
6541317196L-asparagine+assimilation
6541318050L-glutamine+assimilation
6541315603L-leucine+assimilation
6541318019L-lysine+assimilation
6541317295L-phenylalanine+assimilation
6541328808mannan-assimilation
6541317632nitrate-reduction
6541316634raffinose-assimilation
6541353426tween 80+assimilation

metabolite production

  • @ref: 65413
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
65413catalase+1.11.1.6
65413cytochrome oxidase+1.9.3.1
65413arginine dihydrolase-3.5.3.6
65413urease-3.5.1.5
65413protease-
65413cystine arylamidase-3.4.11.3
65413trypsin-3.4.21.4
65413chymotrypsin-3.4.4.5
65413naphthol-AS-BI-phosphohydrolase-
65413N-acetyl-beta-glucosaminidase-3.2.1.52
65413alpha-mannosidase-3.2.1.24
65413alpha-fucosidase-3.2.1.51
65413alpha-glucosidase+3.2.1.20
65413beta-glucosidase+3.2.1.21
65413beta-galactosidase+3.2.1.23
65413alkaline phosphatase+3.1.3.1
65413esterase (C 4)+
65413lipase (C 14)+
65413leucine arylamidase+3.4.11.1
65413valine arylamidase+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
64935moribund turbot larvae from rearing unitsSpainESPEurope
65413a Spanish turbot-rearing unit.SpainESPEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Aquaculture
#Engineered#Agriculture
#Host#Fishes

taxonmaps

  • @ref: 69479
  • File name: preview.99_1899.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_19;96_355;97_566;98_1495;99_1899&stattab=map
  • Last taxonomy: Phaeobacter
  • 16S sequence: AJ536669
  • Sequence Identity:
  • Total samples: 7469
  • soil counts: 318
  • aquatic counts: 6774
  • animal counts: 295
  • plant counts: 82

Safety information

risk assessment

  • @ref: 64935
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessiondatabaselengthNCBI tax ID
6541316S rRNA gene sequenceAJ536669.1nuccore
64935Phaeobacter sp. 27-4 partial 16S rRNA gene, isolate 27-4AJ536669ena14521580596

GC content

@refGC-contentmethod
6493560.1sequence analysis
6541360.1

External links

@ref: 64935

culture collection no.: DSM 103509, LMG 29708

straininfo link

  • @ref: 111093
  • straininfo: 397555

literature

  • topic: Phylogeny
  • Pubmed-ID: 28984543
  • title: Phaeobacter piscinae sp. nov., a species of the Roseobacter group and potential aquaculture probiont.
  • authors: Sonnenschein EC, Phippen CBW, Nielsen KF, Mateiu RV, Melchiorsen J, Gram L, Overmann J, Freese HM
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002331
  • year: 2017
  • mesh: Animals, *Aquaculture, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Denmark, Fatty Acids/chemistry, Fishes, Greece, Heterotrophic Processes, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Spain, Tropolone/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64935Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-103509Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103509)
65413Eva Christina Sonnenschein, Christopher Broughton William Phippen, Kristian Fog Nielsen, Ramona Valentina Mateiu, Jette Melchiorsen, Lone Gram, Jörg Overmann, Heike M. FreesePhaeobacter piscinae sp. nov., a species of the Roseobacter group and potential aquaculture probiont10.1099/ijsem.0.002331IJSEM 67: 4559-4564 201728984543
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
111093Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397555.1