Strain identifier

BacDive ID: 158264

Type strain: Yes

Species: Mycobacterium grossiae

Strain Designation: PB739

Strain history: CIP <- 2017, R. Vasireddy, Texas Univ., Tyler, Texas, USA: strain PB739 <- 2013, L. Ladutko, VA Connecticut Healthcare, West Haven, Connecticut, USA

NCBI tax ID(s): 1552759 (species)

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General

@ref: 64923

BacDive-ID: 158264

DSM-Number: 104744

keywords: genome sequence, 16S sequence, Bacteria, Gram-positive, rod-shaped, human pathogen

description: Mycobacterium grossiae PB739 is a Gram-positive, rod-shaped human pathogen that was isolated from sputum of a 76-year-old patient with severe chronic obstructive pulmonary disease.

NCBI tax id

  • NCBI tax id: 1552759
  • Matching level: species

strain history

@refhistory
64923<- R. Vasireddy; The University of Texas Health Science Center at Tyler, Tyler, TX, USA; PB739<-R. J. Wallace Jr.<-VA Connecticut Healthcare System; VA Connecticut Healthcare System, West Haven, CT, USA
119748CIP <- 2017, R. Vasireddy, Texas Univ., Tyler, Texas, USA: strain PB739 <- 2013, L. Ladutko, VA Connecticut Healthcare, West Haven, Connecticut, USA

doi: 10.13145/bacdive158264.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium grossiae
  • full scientific name: Mycobacterium grossiae Paniz-Mondolfi et al. 2017

@ref: 64923

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium grossiae

full scientific name: Mycobacterium grossiae Paniz-Mondolfi et al. 2017

strain designation: PB739

type strain: yes

Morphology

cell morphology

  • @ref: 119748
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 65412
  • colony color: dark orange/yellow
  • incubation period: 5 days
  • medium used: 5 % sheep blood agar, Middlebrook 7H10 agar and egg-based Lowenstein-Jensen slants

multimedia

  • @ref: 64923
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_104744.jpg
  • caption: Medium 250 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
64923MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
64923PEPTONE MEAT EXTRACT GLYCEROL AGAR (DSMZ Medium 250)yeshttps://mediadive.dsmz.de/medium/250Name: PEPTONE MEAT EXTRACT GLYCEROL AGAR (DSMZ Medium 250) Composition: Agar 20.0 g/l Proteose peptone no. 3 5.0 g/l Meat extract 3.0 g/l Glycerol Distilled water
654125 % sheep blood agar, Middlebrook 7H10 agar and egg-based Lowenstein-Jensen slantsyes
119748CIP Medium 93yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=93

culture temp

@refgrowthtypetemperature
64923positivegrowth37
65412positivegrowth24-42
65412positiveoptimum37

Physiology and metabolism

spore formation

  • @ref: 69481
  • spore formation: no
  • confidence: 96

observation

@refobservation
65412growth is observed on MacConkey agar medium without crystal violet, and no growth is observed with 5 % NaCl
65412species was isolated from two different clinical sources suggesting its role as an emerging human pathogen

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6541253426tween 80+hydrolysis
6836925115malate+assimilation
6836927689decanoate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
683695291gelatin+hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
65412nitrate reductase+1.7.99.4
65412urease+3.5.1.5
65412arylsulfatase-3.1.6.1
68369gelatinase+
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
64923-+++-+++/---+/-----+/-----

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
64923----++/-++/-+/---+/---+/--+/-++/--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeisolation date
64923sputum of a 76-year-old patient with severe chronic obstructive pulmonary diseaseWest Haven, Connecticut (41.2671° N, 72.9475° W)USAUSANorth America41.2671-72.9475
65412a human pulmonary specimenUSAUSANorth America
119748Human, SputumWest Haven, Connecticut, USAUnited States of AmericaUSANorth America2013-03-02

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Fluids#Sputum
#Infection#Disease
#Infection#Patient

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
64923yes, in single cases1Risk group (German classification)
1197481Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessiondatabaselengthNCBI tax ID
65412Mycolicibacterium grossiae heat shock protein 65 (hsp65) gene, partial cdsKM186138nuccore
65412Mycolicibacterium grossiae RNA polymerase beta subunit (rpoB) gene, partial cdsKM186139nuccore
64923Mycolicibacterium grossiae 16S ribosomal RNA gene, partial sequenceKM186137nuccore14921552759

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium grossiae DSM 104744GCA_008329645completencbi1552759
66792Mycobacterium grossiae strain DSM 1047441552759.3completepatric1552759

GC content

  • @ref: 64923
  • GC-content: 70.0
  • method: sequence analysis

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno96no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes88.349no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no98.21no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes88.359no
69480spore-formingspore-formingAbility to form endo- or exosporesno59.634no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno96.167no
69480flagellatedmotile2+Ability to perform flagellated movementno87.371no

External links

@ref: 64923

culture collection no.: DSM 104744, CIP 111318

straininfo link

  • @ref: 111082
  • straininfo: 407234

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28984546Mycobacterium grossiae sp. nov., a rapidly growing, scotochromogenic species isolated from human clinical respiratory and blood culture specimens.Paniz-Mondolfi AE, Greninger AL, Ladutko L, Brown-Elliott BA, Vasireddy R, Jakubiec W, Vasireddy S, Wallace RJ Jr, Simmon KE, Dunn BE, Jackoway G, Vora SB, Quinn KK, Qin X, Campbell SInt J Syst Evol Microbiol10.1099/ijsem.0.0022162017Adolescent, Aged, Bacterial Typing Techniques, Blood Culture, DNA, Bacterial/genetics, Humans, Male, Multilocus Sequence Typing, Mycobacterium/*classification/genetics/isolation & purification, Mycobacterium Infections/blood/*microbiology, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sputum/microbiologyPathogenicity
Phylogeny31907602Horizontal Gene Transfer of Short-Chain Dehydrogenase Coding Genes Contribute to the Biofilm Formation and Pathogenicity on Mycobacterium grossiae sp. nov. PB739(T) (=DSM 104744(T)).Chen G, Song W, Ying XCurr Microbiol10.1007/s00284-019-01832-32020Aged, Base Composition, Biofilms/*growth & development, DNA, Bacterial/genetics, *Gene Transfer, Horizontal, Genome, Bacterial, Genomics, Humans, Male, Mycobacterium/enzymology/*genetics/*pathogenicity, Mycobacterium Infections/microbiology, Oxidoreductases/*genetics, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, VirulenceGenetics

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64923Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-104744Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 104744)
65412Alberto Enrique Paniz-Mondolfi, Alexander L. Greninger, Lynn Ladutko, Barbara A. Brown-Elliott, Ravikiran Vasireddy, Wesley Jakubiec, Sruthi Vasireddy, Richard J. Wallace Jr, Keith E. Simmon, Bruce E. Dunn, Gary Jackoway, Surabhi B. Vora, Kevin K. Quinn, Xuan Qin, Sheldon CampbellMycobacterium grossiae sp. nov., a rapidly growing, scotochromogenic species isolated from human clinical respiratory and blood culture specimens10.1099/ijsem.0.002216IJSEM 67: 4345-4351 201728984546
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
111082Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407234.1
119748Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20111318Collection of Institut Pasteur (CIP 111318)