Strain identifier

BacDive ID: 1582

Type strain: Yes

Species: Ezakiella coagulans

Strain Designation: EUH 581-73

Strain history: LMG 8206 <-- CCUG 10974 <-- ATCC 29798 <-- E. Cato VPI 8755 <-- D. Lambe EUH 581-73.

NCBI tax ID(s): 46507 (species)

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General

@ref: 8782

BacDive-ID: 1582

DSM-Number: 20705

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive

description: Ezakiella coagulans EUH 581-73 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from perineum scar.

NCBI tax id

  • NCBI tax id: 46507
  • Matching level: species

strain history

@refhistory
8782<- E.P. Cato (VPI 8755) <- D.W. Lambe, Jr., EUH 581-73
67770LMG 8206 <-- CCUG 10974 <-- ATCC 29798 <-- E. Cato VPI 8755 <-- D. Lambe EUH 581-73.

doi: 10.13145/bacdive1582.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Peptoniphilaceae
  • genus: Ezakiella
  • species: Ezakiella coagulans
  • full scientific name: Ezakiella coagulans (Eggerth and Gagnon 1933) García-López et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacteroides coagulans

@ref: 8782

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Bacteroidales

family: Peptoniphilaceae

genus: Ezakiella

species: Ezakiella coagulans

full scientific name: Ezakiella coagulans (Eggerth and Gagnon 1933) García-López et al. 2020

strain designation: EUH 581-73

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8782COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8782CHOPPED MEAT MEDIUM (DSMZ Medium 78)yeshttps://mediadive.dsmz.de/medium/78Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water

culture temp

@refgrowthtypetemperaturerange
8782positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
8782anaerobe
69480anaerobe99.171

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.998

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan+energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68380serine arylamidase+
68380glutamyl-glutamate arylamidase+
68380glycin arylamidase+
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase+3.4.19.3
68380leucine arylamidase+3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase+4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8782-+-------------+++++/-+/-++++++/-++

Isolation, sampling and environmental information

isolation

@refsample type
8782perineum scar
67770Human feces

isolation source categories

Cat1Cat2
#Host Body-Site#Gastrointestinal tract
#Host Body-Site#Urogenital tract

taxonmaps

  • @ref: 69479
  • File name: preview.99_1327.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15819;96_769;97_897;98_1051;99_1327&stattab=map
  • Last taxonomy: Ezakiella coagulans subclade
  • 16S sequence: HF558387
  • Sequence Identity:
  • Total samples: 69612
  • soil counts: 1428
  • aquatic counts: 1504
  • animal counts: 66211
  • plant counts: 469

Safety information

risk assessment

  • @ref: 8782
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bacteroides coagulans 16S ribosomal RNA gene, partial sequenceU5041859ena46507
20218Bacteroides coagulans partial 16S rRNA gene, strain EUH 581-73HF5583871473ena46507
8782[Bacteroides] coagulans gene for 16S ribosomal RNA, partial sequence, strain: JCM 12528AB5476391489ena46507

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Ezakiella coagulans DSM 20705GCA_003096635scaffoldncbi46507
66792[Bacteroides] coagulans strain DSM 2070546507.4wgspatric46507
66792Bacteroides coagulans DSM 207052756170280draftimg46507

GC content

@refGC-contentmethod
6777037thermal denaturation, midpoint method (Tm)
6777035.6genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno79no
flagellatedno96.982no
gram-positiveyes86.019no
anaerobicyes98.968yes
halophileno78.836no
spore-formingno83.708no
glucose-utilyes83.69no
aerobicno97.486yes
thermophileno90.797no
motileno90.996no
glucose-fermentyes54.182no

External links

@ref: 8782

culture collection no.: DSM 20705, ATCC 29798, JCM 12528, CCUG 10974, CCUG 48292, KCTC 5205, LMG 8206

straininfo link

  • @ref: 71227
  • straininfo: 1211

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8782Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20705)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20705
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
71227Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID1211.1StrainInfo: A central database for resolving microbial strain identifiers