Strain identifier
BacDive ID: 158131
Type strain: 
Species: Brucella haematophila
Strain Designation: LMH00001, UHS000020, P6BS-III
Strain history: <- S. Thijs, Hasselt Univ., Diepenbeek, Belgium; P6BS-III
NCBI tax ID(s): 419474 (species)
version 9 (current version)
General
@ref: 64710
BacDive-ID: 158131
DSM-Number: 106071
keywords: Bacteria
description: Brucella haematophila LMH00001 is a bacterium that was isolated from soil, pasturelands chronically exposed to glyphosate.
NCBI tax id
- NCBI tax id: 419474
- Matching level: species
strain history
- @ref: 64710
- history: <- S. Thijs, Hasselt Univ., Diepenbeek, Belgium; P6BS-III
doi: 10.13145/bacdive158131.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Brucellaceae
- genus: Brucella
- species: Brucella haematophila
- full scientific name: Brucella haematophila (Kämpfer et al. 2007) Hördt et al. 2020
synonyms
- @ref: 20215
- synonym: Ochrobactrum haematophilum
@ref: 64710
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Brucellaceae
genus: Brucella
species: Brucella haematophila
full scientific name: Brucella haematophila (Kämpfer et al. 2007) Hördt et al. 2020
strain designation: LMH00001, UHS000020, P6BS-III
type strain: no
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 64710 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
| 64710 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
culture temp
- @ref: 64710
- growth: positive
- type: growth
- temperature: 37
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | + | assimilation |
| 68369 | 27689 | decanoate | - | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | + | assimilation |
| 68369 | 59640 | N-acetylglucosamine | + | assimilation |
| 68369 | 16899 | D-mannitol | + | assimilation |
| 68369 | 16024 | D-mannose | + | assimilation |
| 68369 | 30849 | L-arabinose | + | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | + | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | + | 3.2.1.21 |
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 64710 | - | - | - | - | - | + | - | - | + | + | + | + | + | + | + | - | + | + | + | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 64710
- sample type: soil, pasturelands chronically exposed to glyphosate
- geographic location: Buenos Aires, Chascomús, Humid Pampa (35° 44' 39.26'' S, 58° 3' 27.33'' W)
- country: Argentina
- origin.country: ARG
- continent: Middle and South America
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
- @ref: 64710
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
External links
@ref: 64710
culture collection no.: DSM 106071
straininfo link
- @ref: 110967
- straininfo: 406838
literature
- Pubmed-ID: 30800585
- title: First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina.
- authors: Massot F, Gkorezis P, McAmmond B, d'Haen J, Van Hamme J, Merini LJ, Vangronsveld J, Thijs S
- journal: 3 Biotech
- DOI: 10.1007/s13205-019-1606-y
- year: 2019
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 64710 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-106071 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106071) | |
| 68369 | Automatically annotated from API 20NE | |||
| 110967 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID406838.1 |