Strain identifier

BacDive ID: 157895

Type strain: Yes

Species: Streptomyces roseifaciens

Strain history: <- L. van der Aart, Leiden Univ.Inst. of Biology, Netherlands; MBT76 <- H. Zhu

NCBI tax ID(s): 1488406 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 8.1 (current version)

General

@ref: 64468

BacDive-ID: 157895

DSM-Number: 106196

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, pigmented

description: Streptomyces roseifaciens DSM 106196 is an aerobe, spore-forming, mesophilic bacterium that has a pale brown pigmentation and was isolated from soil.

NCBI tax id

  • NCBI tax id: 1488406
  • Matching level: species

strain history

  • @ref: 64468
  • history: <- L. van der Aart, Leiden Univ.Inst. of Biology, Netherlands; MBT76 <- H. Zhu

doi: 10.13145/bacdive157895.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces roseifaciens
  • full scientific name: Streptomyces roseifaciens van der Aart et al. 2019

@ref: 64468

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces roseifaciens

full scientific name: Streptomyces roseifaciens van der Aart et al. 2019

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
67823positive
69480no93.307
69480positive100

colony morphology

@refcolony colormedium used
69308Chocolate brown (8017)ISP 2
69308Grey brown (8019), Olive brown (8008)ISP 6
69308Ochre brown (8001), Nut brown (8011), Strawberry red (3018)ISP 4
69308Pale brown (8025), Nut brown (8011)suter without tyrosine
69308Sepia brown (8014), Green brown (8000)ISP 7
69308Sepia brown (8014), Nut brown (8011)suter with tyrosine
69308Sepia brown (8014), Ruby red (3003), Rose (3017)ISP 3
69308Sepia brown (8014), Sand yellow (1002)ISP 5

multicellular morphology

@refforms multicellular complexcomplex colormedium namefurther descriptioncomplex name
67823yesredoatmeal agarextensively branched substrate mycelium that carries long straight filaments bearing at more or lessregular intervals branches arranged in verticils. Eachbranch of the verticils produces at its apex short chainsof three to five spores with smooth surfaces. Grows well on all ISP media. A red substrate mycelium, a pinkaerial spore mass are produced.
69308yesLight grey (7035)ISP 2Aerial mycelium
69308yesGreen beige (1000), signal grey (9003), light pink (3015)ISP 3Aerial mycelium
69308yesSignal white (9003)ISP 4Aerial mycelium
69308noISP 5Aerial mycelium
69308noISP 6Aerial mycelium
69308yesLight pink (3015), light grey (7035)ISP 7Aerial mycelium
69308nosuter with tyrosineAerial mycelium
69308nosuter without tyrosineAerial mycelium

pigmentation

@refproductioncolorname
67823yespale brown
69308noMelanin
69308yesFawn brown (8007), brown beige (1011), sand yellow (1002), ochre brown (8001), mahogany brown (8016)soluble pigment

multimedia

  • @ref: 64468
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_106196.jpg
  • caption: Medium 252 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
64468STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
64468GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
64468positivegrowth28mesophilic
67823positivegrowth20-50
67823positiveoptimum30mesophilic

culture pH

@refabilitytypepHPH range
67823positivegrowth5-11alkaliphile
67823positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 67823
  • oxygen tolerance: aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
67823short chains of three to five spores with smooth surfaces, pinkaerial spore masssporeyes
69480yes100
69481yes100

halophily

@refsaltgrowthtested relationconcentration
67823NaClpositivegrowth2 %
67823NaClnogrowth3 %(w/v)
69308NaClpositivegrowth0-2.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6930822599arabinose-growth
67823casein+degradation
678235291gelatin+degradation
6782317368hypoxanthine+degradation
6782328017starch+degradation
6782317895L-tyrosine+degradation
6782315318xanthine-degradation
6782317234glucose+carbon source
6782317268myo-inositol+carbon source
6782317992sucrose+carbon source
6782328757fructose-carbon source
6930862968cellulose-growth
6930828757fructose-growth
6930817234glucose+growth
6930817268inositol+/-growth
6930837684mannose-growth
6930816634raffinose-growth
6930826546rhamnose-growth
6930817992sucrose-growth
6930818222xylose-growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
67823acid phosphatase+3.1.3.2
67823alkaline phosphatase+3.1.3.1
67823alpha-chymotrypsin+3.4.21.1
67823cystine arylamidase+3.4.11.3
67823esterase (C 4)+
67823esterase Lipase (C 8)+
67823N-acetyl-beta-glucosaminidase+3.2.1.52
67823alpha-glucosidase+3.2.1.20
67823beta-glucosidase+3.2.1.21
67823alpha-mannosidase+3.2.1.24
67823naphthol-AS-BI-phosphohydrolase+
67823trypsin+3.4.21.4
67823valine arylamidase+
67823alpha-fucosidase-3.2.1.51
67823alpha-galactosidase-3.2.1.22
67823beta-galactosidase-3.2.1.23
67823beta-glucuronidase-3.2.1.31
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382trypsin+3.4.21.4
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    67823C15:0 anteiso34.4
    67823C17:0 anteiso10.92
    67823C14:0 iso8.28
    67823C15:0 iso5.11
    67823C16:0 iso7.99
    67823C16:0 anteiso2.54
    67823C16:1 w9c2.84
    67823C16:05.64
    67823C18:1 w9c8.93
    67823C20:11 w11c4.53
    67823C18:2 w9,12/C18:08.81
  • type of FA analysis: whole cell analysis
  • incubation medium: ISP2 broth
  • agar/liquid: liquid
  • incubation temperature: 30
  • incubation time: 2
  • library/peak naming table: ACTINO 6
  • system: MIS MIDI

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69308--++---+--+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69308++++/-++/-+/-++/-++-----++/--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
64468soilQuinLing mountains, Xi'an, Shaanxi Province (34° 3' 28'' N, 109° 22' 38.9'' E)ChinaCHNAsia34.0578109.378
67823soil sample (depth 10-20 cm)Shandi Village, QinLing mountains, Shaanxi ProvinceChinaCHNAsia34.4983109.378selective agarsupplemented with nystatin (50 µg ml-1) and nalidixic acid (10 mg ml-1)4 days30soil sample (1 g) was enriched with 6 % yeast extract broth and incubated at 37°C for 2 h in a shaking incubator. Aliquots (0.1 ml) of 10-2 to 10-4 dilutions of the resultant preparations were spread over selective agar plates that were incubated at 30°C for 4 days.

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Sequence information

16S sequences

  • @ref: 64468
  • accession: GCA_001445655.1
  • database: ena

Genome sequences

  • @ref: 67823
  • description: Streptomyces roseifaciens MBT76
  • accession: GCA_001445655
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 1488406

GC content

  • @ref: 67823
  • GC-content: 71.9
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedno98.165no
gram-positiveyes89.446yes
anaerobicno99.285yes
aerobicyes91.125yes
halophileno94.058no
spore-formingyes94.193yes
glucose-utilyes90.87yes
motileno93.752no
thermophileno98.488yes
glucose-fermentno91.492yes

External links

@ref: 64468

culture collection no.: DSM 106196, NCCB 100637, MBT 76

straininfo link

  • @ref: 110750
  • straininfo: 403655

literature

  • topic: Phylogeny
  • Pubmed-ID: 30625109
  • title: Polyphasic classification of the gifted natural product producer Streptomyces roseifaciens sp. nov.
  • authors: van der Aart LT, Nouioui I, Kloosterman A, Igual JM, Willemse J, Goodfellow M, van Wezel GP
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003215
  • year: 2019
  • mesh: Bacterial Typing Techniques, Base Composition, Biological Products, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Multilocus Sequence Typing, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64468Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-106196Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106196)
67823Lizah T. van der Aart, Imen Nouioui, Alexander Kloosterman, José-Mariano Igual, Joost Willemse, Michael Goodfellow, Gilles P. van WezelPolyphasic classification of the gifted natural product producer Streptomyces roseifaciens sp. nov.10.1099/ijsem.0.003215IJSEM 69: 899-908 2019
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69308Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%20106196.pdf
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
110750Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403655.1