Strain identifier
BacDive ID: 1545
Type strain: 
Species: Piscibacillus halophilus
Strain Designation: HS224
Strain history: A. Ventosa HS 224.
NCBI tax ID(s): 571933 (species)
version 8.1 (current version)
General
@ref: 15849
BacDive-ID: 1545
DSM-Number: 21633
keywords: genome sequence, 16S sequence, Bacteria, mesophilic
description: Piscibacillus halophilus HS224 is a mesophilic bacterium that was isolated from hypersaline lake water.
NCBI tax id
- NCBI tax id: 571933
- Matching level: species
strain history
| @ref | history |
|---|---|
| 15849 | <- C. Sánchez-Porro, Universidad de Alcalá de Henares, Madrid; HS224 <- M. A. Amoozegar and R. Rohban |
| 67770 | A. Ventosa HS 224. |
doi: 10.13145/bacdive1545.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Piscibacillus
- species: Piscibacillus halophilus
- full scientific name: Piscibacillus halophilus Amoozegar et al. 2009
@ref: 15849
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Piscibacillus
species: Piscibacillus halophilus
full scientific name: Piscibacillus halophilus Amoozegar et al. 2009
strain designation: HS224
type strain: yes
Culture and growth conditions
culture medium
- @ref: 15849
- name: MODIFIED BACTO MARINE BROTH (DSMZ Medium 514d)
- growth: yes
- link: https://mediadive.dsmz.de/medium/514d
- composition: Name: MODIFIED BACTO MARINE BROTH (DSMZ Medium 514d; with strain-specific modifications) Composition: NaCl 100.0 g/l Difco marine broth 37.4 g/l Agar 15.0 g/l Malt extract 1.0 g/l Soy peptone 1.0 g/l Pancreatic digest of casein 1.0 g/l MnSO4 0.01 g/l Distilled water
culture temp
- @ref: 67770
- growth: positive
- type: growth
- temperature: 34
- range: mesophilic
Physiology and metabolism
observation
- @ref: 67770
- observation: quinones: MK-7
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 15849 | hypersaline lake water | lake Howz-Soltan | Iran | IRN | Asia |
| 67770 | Hypersaline lake Howz-Soltan in Iran |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Lake (large) |
| #Condition | #Saline |
Safety information
risk assessment
- @ref: 15849
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 15849
- description: Piscibacillus halophilus partial 16S rRNA gene, strain HS224
- accession: FM864227
- length: 1472
- database: ena
- NCBI tax ID: 571933
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Piscibacillus halophilus strain DSM 21633 | 571933.3 | wgs | patric | 571933 |
| 66792 | Piscibacillus halophilus DSM 21633 | 2654587883 | draft | img | 571933 |
| 67770 | Piscibacillus halophilus DSM 21633 | GCA_900110685 | contig | ncbi | 571933 |
GC content
- @ref: 15849
- GC-content: 37.5
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| gram-positive | yes | 90.799 | no |
| anaerobic | no | 99.067 | no |
| halophile | yes | 94.511 | no |
| spore-forming | yes | 91.969 | no |
| glucose-util | yes | 87.434 | no |
| flagellated | yes | 61.169 | no |
| aerobic | yes | 95.72 | no |
| thermophile | no | 94.469 | yes |
| motile | yes | 89.817 | no |
| glucose-ferment | no | 92.457 | no |
External links
@ref: 15849
culture collection no.: DSM 21633, CCM 7596, JCM 15721, LMG 24786
straininfo link
- @ref: 71190
- straininfo: 359344
literature
- topic: Phylogeny
- Pubmed-ID: 19643892
- title: Piscibacillus halophilus sp. nov., a moderately halophilic bacterium from a hypersaline Iranian lake.
- authors: Amoozegar MA, Sanchez-Porro C, Rohban R, Hajighasemi M, Ventosa A
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.012013-0
- year: 2009
- mesh: Bacillaceae/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Fresh Water/*microbiology, Iran, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/*metabolism
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 15849 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21633) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21633 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 71190 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID359344.1 | StrainInfo: A central database for resolving microbial strain identifiers |