Strain identifier

BacDive ID: 15327

Type strain: Yes

Species: Streptomyces indiaensis

Strain Designation: MB-T 10

Strain history: KCC A-0053 <-- T. Furumai <-- K. C. Gupta.

NCBI tax ID(s): 284033 (species)

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General

@ref: 11256

BacDive-ID: 15327

DSM-Number: 43803

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces indiaensis MB-T 10 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 284033
  • Matching level: species

strain history

@refhistory
11256<- G. Vobis, MB-T 10 <- T. Frumai, MCRL <- K.C. Gupta, RRL (Streptosporangium indianensis)
67770KCC A-0053 <-- T. Furumai <-- K. C. Gupta.

doi: 10.13145/bacdive15327.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces indiaensis
  • full scientific name: Streptomyces indiaensis (Gupta 1965) Kudo and Seino 1987

  • synonyms

    @refsynonym
    20215Streptosporangium indianense
    20215Streptosporangium indianensis

@ref: 11256

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces indiaensis

full scientific name: Streptomyces indiaensis (Gupta 1965) Kudo and Seino 1987

strain designation: MB-T 10

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19589Dark red10-14 daysISP 2
19589Red10-14 daysISP 3
19589Dark red10-14 daysISP 4
19589Dark red10-14 daysISP 5
19589Brown gray10-14 daysISP 6
19589Dark red10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19589yesAerial MyceliumGrayISP 2
19589yesAerial MyceliumGrayISP 3
19589yesAerial MyceliumGrayISP 4
19589yesAerial MyceliumGrayISP 5
19589noISP 6
19589yesAerial MyceliumGrayISP 7

multimedia

  • @ref: 11256
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43803.jpg
  • caption: Medium 252 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11256GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19589ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19589ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19589ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19589ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19589ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19589ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
11256ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp

@refgrowthtypetemperaturerange
11256positivegrowth28mesophilic
19589positiveoptimum28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19589
  • spore description: Formation of spore chains (spirales)(Sp), spore surface smooth
  • type of spore: spore
  • spore formation: yes

halophily

  • @ref: 19589
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1958917234glucose+
1958922599arabinose+
1958917992sucrose+
1958918222xylose-
1958917268myo-inositol+
1958929864mannitol+
1958928757fructose+
1958926546rhamnose+
1958916634raffinose-
1958962968cellulose-
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19589+++++++--++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
11256soilIndiaINDAsia
67770SoilIndiaINDAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
112561Risk group (German classification)
195891Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces indiaensis gene for 16S rRNA, partial sequence, strain: NBRC 13964AB1845531471ena284033
67770Streptomyces indiaensis 16S rRNA gene, type strain LMG 19961AJ7813441478ena284033

Genome sequences

  • @ref: 66792
  • description: Streptomyces indiaensis DSM 43803
  • accession: GCA_021474405
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 284033

GC content

  • @ref: 67770
  • GC-content: 73.7
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno97.866no
gram-positiveyes89.582no
anaerobicno99.146no
aerobicyes90.808no
halophileno93.631no
spore-formingyes94.87yes
motileno93.347no
glucose-fermentno89.201no
thermophileno98.055no
glucose-utilyes90.522yes

External links

@ref: 11256

culture collection no.: DSM 43803, ATCC 33330, CBS 560.75, IFO 13964, JCM 3053, KCC A-0053, NBRC 13964, CGMCC 4.1952, KCTC 9489, NCIMB 9794, NRRL B-24311

straininfo link

  • @ref: 84420
  • straininfo: 389368

literature

  • topic: Phylogeny
  • Pubmed-ID: 28141499
  • title: Streptomyces jeddahensis sp. nov., an oleaginous bacterium isolated from desert soil.
  • authors: Rottig A, Atasayar E, Meier-Kolthoff JP, Sproer C, Schumann P, Schauer J, Steinbuchel A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001839
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Saudi Arabia, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11256Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43803)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43803
19589Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43803.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
84420Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389368.1StrainInfo: A central database for resolving microbial strain identifiers