Strain identifier

BacDive ID: 15222

Type strain: Yes

Species: Streptomyces glaucescens

Strain history: KCC S-0377 <-- IFO 12774 <-- SAJ <-- ISP 5155 <-- INA 8731.

NCBI tax ID(s): 1907 (species)

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General

@ref: 9354

BacDive-ID: 15222

DSM-Number: 40155

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces glaucescens DSM 40155 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 1907
  • Matching level: species

strain history

@refhistory
9354<- E.B. Shirling, ISP <- T.P. Preobrazhenskaya, INA
67770KCC S-0377 <-- IFO 12774 <-- SAJ <-- ISP 5155 <-- INA 8731.

doi: 10.13145/bacdive15222.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces glaucescens
  • full scientific name: Streptomyces glaucescens (Preobrazhenskaya 1957) Pridham et al. 1958 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Actinomyces glaucescens
    20215Streptomyces pharetrae

@ref: 9354

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces glaucescens

full scientific name: Streptomyces glaucescens (Preobrazhenskaya 1957) Pridham et al. 1958

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19413Red10-14 daysISP 2
19413Rose10-14 daysISP 3
19413Yellow10-14 daysISP 4
19413Yellow10-14 daysISP 5
19413Brown10-14 daysISP 6
19413Yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19413yesAerial MyceliumBlue greyISP 2
19413yesAerial MyceliumWhite greyISP 3
19413yesAerial MyceliumBlue greyISP 4
19413yesAerial MyceliumBlue greyISP 5
19413noISP 6
19413yesAerial MyceliumBlue greyISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9354GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19413ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19413ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19413ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19413ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19413ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19413ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
19413positiveoptimum28mesophilic
9354positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19413
  • spore description: Formation of spore chains: spira, spore surface: warty to hairy
  • spore formation: yes

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1941316634raffinose-
1941326546rhamnose+
1941328757fructose+/-
1941329864mannitol+/-
1941317268myo-inositol-
1941318222xylose-
1941317992sucrose-
1941322599arabinose+
1941317234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase+4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19413++++++++-++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountry
9354soil
67770SoilCaucasusUSSR

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
93541Risk group (German classification)
194131Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces glaucescens strain DSM 40155 16S ribosomal RNA gene, partial sequenceGU383148780ena1907
20218Streptomyces glaucescens gene for 16S ribosomal RNA, partial sequence, strain: JCM 4377D44092121ena1907
20218Streptomyces glaucescens gene for 16S rRNA, partial sequence, strain: NBRC 12774AB1848431477ena1907
20218Streptomyces glaucescens strain NBRC 12774 16S ribosomal RNA gene, partial sequenceJQ899207653ena1907
20218Streptomyces glaucescens strain NBRC 12774 16S ribosomal RNA gene, partial sequenceJQ899216643ena1907
20218Streptomyces glaucescens strain NRRL B-2706T 16S ribosomal RNA gene, partial sequenceDQ4425021407ena1907

Genome sequences

  • @ref: 67770
  • description: Streptomyces glaucescens NRRL B-2706
  • accession: GCA_002154375
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 1907

GC content

  • @ref: 67770
  • GC-content: 72.5
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno98.412no
gram-positiveyes88.615no
anaerobicno99.018no
halophileno91.389no
spore-formingyes94.888yes
glucose-utilyes90.256yes
aerobicyes93.166no
thermophileno98.417yes
motileno94.458no
glucose-fermentno90.44no

External links

@ref: 9354

culture collection no.: DSM 40155, ATCC 19761, ATCC 23622, CBS 499.68, DSM 41504, ETH 24204, ETH A3080, IFO 12774, INA 8731, ISP 5155, JCM 4377, KCC S-0377, NBRC 12774, NCIB 9619, NCIB 9844, RIA 1041, BCRC 11478, CBS 261.66, CECT 3133, CGMCC 4.1408, IMET 43584, KCTC 9881, LMG 19330, MTCC 276, NBIMCC 1638, NCIMB 9619, NCIMB 9844, NRRL B-2706, VKM Ac-617

straininfo link

  • @ref: 84319
  • straininfo: 14505

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9354Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40155)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40155
19413Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40155.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
84319Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID14505.1StrainInfo: A central database for resolving microbial strain identifiers