Strain identifier

BacDive ID: 15107

Type strain: Yes

Species: Streptomyces coerulescens

Strain Designation: 4562

Strain history: <- E.B. Shirling, ISP <- T.P. Preobrazhenskaya, INA, 4562

NCBI tax ID(s): 29304 (species)

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General

@ref: 9346

BacDive-ID: 15107

DSM-Number: 40146

keywords: 16S sequence, Bacteria, mesophilic, antibiotic compound production

description: Streptomyces coerulescens 4562 is a mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 29304
  • Matching level: species

strain history

  • @ref: 9346
  • history: <- E.B. Shirling, ISP <- T.P. Preobrazhenskaya, INA, 4562

doi: 10.13145/bacdive15107.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces coerulescens
  • full scientific name: Streptomyces coerulescens (Preobrazhenskaya 1957) Pridham et al. 1958 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Actinomyces coerulescens

@ref: 9346

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces coerulescens

full scientific name: Streptomyces coerulescens (Preobrazhenskaya 1957) Pridham et al. 1958

strain designation: 4562

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19409Brown beige10-14 daysISP 2
19409Brown beige10-14 daysISP 3
19409Brown beige10-14 daysISP 4
19409Beige10-14 daysISP 5
19409Brown beige10-14 daysISP 6
19409Brown beige10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19409yesAerial MyceliumPale greenISP 2
19409yesAerial MyceliumPale greenISP 3
19409yesAerial MyceliumPale greenISP 4
19409noISP 5
19409noISP 6
19409noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9346GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19409ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19409ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19409ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19409ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19409ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19409ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
19409positiveoptimum28mesophilic
9346positivegrowth28mesophilic

Physiology and metabolism

compound production

@refcompound
9346coerulomycin
20216Coerulomycin

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1940962968cellulose+
1940916634raffinose+
1940926546rhamnose+
1940928757fructose+
1940929864mannitol+
1940917268myo-inositol+
1940918222xylose-
1940917992sucrose+
1940922599arabinose+
1940917234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19409+++-++-++++++-++++-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19409+++++-+--++

Isolation, sampling and environmental information

isolation

  • @ref: 9346
  • sample type: soil in hot climate

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Climate#Hot

Safety information

risk assessment

  • @ref: 9346
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces coerulescens strain AS 4.1597 16S ribosomal RNA gene, partial sequenceFJ4061141358ena29304
20218Streptomyces coerulescens partial 16S rRNA gene, strain ISP 5146AJ3994621450ena29304
20218Streptomyces coerulescens strain ISP 5146 16S ribosomal RNA gene, partial sequenceAY9997201450ena29304
20218Streptomyces coerulescens gene for 16S ribosomal RNA, partial sequence, strain: JCM 4360D44077120ena29304
20218Streptomyces coerulescens gene for 16S rRNA, partial sequence, strain: NBRC 12758AB1841221460ena29304

External links

@ref: 9346

culture collection no.: DSM 40146, ATCC 19742, ATCC 19896, CBS 481.68, IFO 12758, ISP 5146, NBRC 12758, RIA 1023

straininfo link

  • @ref: 84213
  • straininfo: 37146

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity22153873Persipeptides A and B, two cyclic peptides from Streptomyces sp. UTMC 1154.Mohammadipanah F, Matasyoh J, Hamedi J, Klenk HP, Laatsch HBioorg Med Chem10.1016/j.bmc.2011.10.0762011Amino Acid Sequence, Bacteria/drug effects, Fungi/drug effects, Magnetic Resonance Spectroscopy, Microbial Sensitivity Tests, Molecular Conformation, Peptides, Cyclic/*chemistry/isolation & purification/pharmacology, Streptomyces/*metabolismEnzymology
Phylogeny25030519Streptomyces zagrosensis sp. nov., isolated from soil.Mohammadipanah F, Hamedi J, Sproer C, Rohde M, Montero-Calasanz MDC, Klenk HPInt J Syst Evol Microbiol10.1099/ijs.0.064527-02014Bacterial Typing Techniques, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Iran, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purificationGenetics
Phylogeny27582375Streptomyces tremellae sp. nov., isolated from a culture of the mushroom Tremella fuciformis.Wen ZQ, Chen B, Li X, Li BB, Li CH, Huang QH, Zhang QH, Dai WH, Jiang YJInt J Syst Evol Microbiol10.1099/ijsem.0.0014642016*Agaricales, Bacterial Typing Techniques, Base Composition, *Basidiomycota, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny28920834Streptomyces xylanilyticus sp. nov., isolated from soil.Moonmangmee D, Kanchanasin P, Phongsopitanun W, Tanasupawat S, Moonmangmee SInt J Syst Evol Microbiol10.1099/ijsem.0.0022822017Bacterial Typing Techniques, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Glycolipids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Thailand, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny34480669Streptomyces endocoffeicus sp. nov., an endophytic actinomycete isolated from Coffea arabica (L.).Nammali A, Intaraudom C, Pittayakhajonwut P, Suriyachadkun C, Tadtong S, Tanasupawat S, Thawai CAntonie Van Leeuwenhoek10.1007/s10482-021-01648-x2021*Actinobacteria, Bacterial Typing Techniques, Base Composition, *Coffea, DNA, Bacterial/genetics, Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Streptomyces/genetics, ThailandTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9346Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40146)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40146
19409Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40146.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
84213Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID37146.1StrainInfo: A central database for resolving microbial strain identifiers