Strain identifier

BacDive ID: 15087

Type strain: Yes

Species: Streptomyces chattanoogensis

Strain history: KCC S-0299 <-- CUB 136 <-- ISP 5002 <-- J. Burns J-23.

NCBI tax ID(s): 66876 (species)

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General

@ref: 9207

BacDive-ID: 15087

DSM-Number: 40002

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, antibiotic compound production

description: Streptomyces chattanoogensis DSM 40002 is a mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 66876
  • Matching level: species

strain history

@refhistory
9207<- E.B. Shirling, ISP <- J. Burns J-23
67770KCC S-0299 <-- CUB 136 <-- ISP 5002 <-- J. Burns J-23.

doi: 10.13145/bacdive15087.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces chattanoogensis
  • full scientific name: Streptomyces chattanoogensis Burns and Holtman 1959 (Approved Lists 1980)

@ref: 9207

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces chattanoogensis

full scientific name: Streptomyces chattanoogensis Burns and Holtman 1959

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19382Maize yellow10-14 daysISP 2
19382Lemon yellow10-14 daysISP 3
19382Lemon yellow10-14 daysISP 4
1938210-14 daysISP 5
19382Beige10-14 daysISP 6
19382Lemon yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
19382yesAerial MyceliumISP 4
19382yesAerial MyceliumISP 2Cream
19382yesAerial MyceliumISP 3Cream
19382yesAerial MyceliumISP 4Cream
19382noISP 5
19382noISP 6
19382noISP 7

multimedia

@refmultimedia contentintellectual property rightscaption
9207https://www.dsmz.de/microorganisms/photos/DSM_40002-1.jpg© Leibniz-Institut DSMZ
9207https://www.dsmz.de/microorganisms/photos/DSM_40002.jpg© Leibniz-Institut DSMZMedium 65 28°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9207GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19382ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19382ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19382ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19382ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19382ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19382ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9207ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
19382positiveoptimum28mesophilic
9207positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

compound production

  • @ref: 9207
  • compound: tennecetin

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1938262968cellulose+
1938216634raffinose-
1938226546rhamnose-
1938228757fructose-
1938229864mannitol+
1938217268myo-inositol-
1938218222xylose-
1938217992sucrose-
1938222599arabinose-
1938217234glucose+
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
677707488natamycinyes
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19382+++++++++++---+-+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19382----+----+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
9207soilTennesseeUSAUSANorth America
67770SoilTNUSAUSANorth America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_357.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_91;97_97;98_106;99_357&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: AY295791
  • Sequence Identity:
  • Total samples: 5402
  • soil counts: 2201
  • aquatic counts: 991
  • animal counts: 1970
  • plant counts: 240

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
92071Risk group (German classification)
193821Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces chattanoogensis strain CGMCC 4.1415 16S ribosomal RNA gene, partial sequenceJN5660191493ena66876
20218Streptomyces chattanoogensis 16S rRNA gene, type strain DSM 40002TAJ6216111484ena66876
20218Streptomyces chattanoogensis 16S ribosomal RNA gene, partial sequenceAY2957911457ena66876
20218Streptomyces chattanoogensis gene for 16S ribosomal RNA, partial sequence, strain: JCM 4299D44047121ena66876
20218Streptomyces chattanoogensis gene for 16S rRNA, partial sequence, strain: NBRC 12754AB1841191459ena66876

Genome sequences

  • @ref: 67770
  • description: Streptomyces chattanoogensis NRRL ISP-5002
  • accession: GCA_001294335
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 66876

GC content

  • @ref: 67770
  • GC-content: 70.9
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno94.078no
flagellatedno97.617no
gram-positiveyes87.592no
anaerobicno99.245no
aerobicyes93.657no
halophileno92.802no
spore-formingyes94.46no
glucose-utilyes90.148yes
thermophileno98.425yes
glucose-fermentno92.262no

External links

@ref: 9207

culture collection no.: DSM 40002, ATCC 13358, ATCC 19739, CBS 477.68, IFO 12754, ISP 5002, JCM 4299, JCM 4571, NBRC 12754, RIA 1019, BCRC 13655, CECT 3321, CGMCC 4.1415, KCTC 1087, LMG 19339, NCIMB 9809, NRRL B-2255, NRRL ISP-5002, VKM Ac-1775

straininfo link

  • @ref: 84193
  • straininfo: 13349

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny21669925Streptomyces staurosporininus sp. nov., a staurosporine-producing actinomycete.Kim BY, Zucchi TD, Fiedler HP, Goodfellow MInt J Syst Evol Microbiol10.1099/ijs.0.031922-02011Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Staurosporine/*biosynthesis, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny27670691Streptomyces kronopolitis sp. nov., an actinomycete that produces phoslactomycins isolated from a millipede (Kronopolites svenhedind Verhoeff).Liu C, Ye L, Li Y, Jiang S, Liu H, Yan K, Xiang W, Wang XInt J Syst Evol Microbiol10.1099/ijsem.0.0015202016Animals, Arthropods/*microbiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny32244447Characterization of Streptomyces piniterrae sp. nov. and Identification of the Putative Gene Cluster Encoding the Biosynthesis of Heliquinomycins.Zhuang X, Wang Z, Peng C, Su C, Gao C, Wang Y, Huang S, Liu CMicroorganisms10.3390/microorganisms80404952020

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9207Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40002)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40002
19382Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40002.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
84193Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13349.1StrainInfo: A central database for resolving microbial strain identifiers