Strain identifier

BacDive ID: 15052

Type strain: Yes

Species: Streptomyces blastmyceticus

Strain Designation: 445 D1, 445-D1

Strain history: CIP <- 2003, JCM <- 1983, KCC <- IFO <- SAJ <- ISP <- H. Yonehara: strain 445-D1

NCBI tax ID(s): 68180 (species)

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General

@ref: 9228

BacDive-ID: 15052

DSM-Number: 40029

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped, antibiotic compound production

description: Streptomyces blastmyceticus 445 D1 is an obligate aerobe, mesophilic, Gram-positive bacterium that produces antibiotic compounds and was isolated from soil.

NCBI tax id

  • NCBI tax id: 68180
  • Matching level: species

strain history

@refhistory
9228<- E.B. Shirling, ISP <- J. Yonehara, 445 D1
67770KCC S-0184 <-- H. Yonehara 455-D1.
116826CIP <- 2003, JCM <- 1983, KCC <- IFO <- SAJ <- ISP <- H. Yonehara: strain 445-D1

doi: 10.13145/bacdive15052.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces blastmyceticus
  • full scientific name: Streptomyces blastmyceticus (Watanabe et al. 1957) Witt and Stackebrandt 1991

  • synonyms

    @refsynonym
    20215Streptoverticillium blastmyceticum
    20215Streptomyces blastmyceticus

@ref: 9228

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces blastmyceticus

full scientific name: Streptomyces blastmyceticus (Watanabe et al. 1957) Witt and Stackebrandt 1991

strain designation: 445 D1, 445-D1

type strain: yes

Morphology

cell morphology

  • @ref: 116826
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 116826

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9228STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
9228ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
38176MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
9228ISP 5 MEDIUM (DSMZ Medium 993)yeshttps://mediadive.dsmz.de/medium/993Name: ISP 5 MEDIUM (DSMZ Medium 993) Composition: Agar 20.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l L-Asparagine 1.0 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water
9228GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
116826CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
18467positiveoptimum28mesophilic
9228positivegrowth28mesophilic
38176positivegrowth30mesophilic
67770positivegrowth28mesophilic
116826positivegrowth25-37mesophilic
116826nogrowth10psychrophilic
116826nogrowth41thermophilic
116826nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116826
  • oxygen tolerance: obligate aerobe

compound production

@refcompound
9228blastmycin (= antimycin A3)
20216Blastmycin (=antimycin A3)

halophily

@refsaltgrowthtested relationconcentration
116826NaClnogrowth0 %
116826NaClnogrowth2 %
116826NaClnogrowth4 %
116826NaClnogrowth6 %
116826NaClnogrowth8 %
116826NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1846717234glucose+
1846722599arabinose+/-
1846717992sucrose+/-
1846718222xylose+
1846717268myo-inositol+
1846729864mannitol+/-
1846728757fructose+
1846726546rhamnose+/-
1846716634raffinose+
1846762968cellulose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis
11682616947citrate-carbon source
1168264853esculin+hydrolysis
116826606565hippurate-hydrolysis
11682617632nitrate-reduction
11682616301nitrite-reduction
11682617632nitrate-respiration

antibiotic resistance

  • @ref: 116826
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refmetaboliteproductionChebi-ID
67770antimycin A3yes
68368acetoinyes15688
68368indoleno35581
68368hydrogen sulfideno16136
116826indoleno35581

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
11682615688acetoin-
11682617234glucose-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase-3.2.1.23
116826oxidase-
116826beta-galactosidase+3.2.1.23
116826alcohol dehydrogenase-1.1.1.1
116826gelatinase+/-
116826amylase-
116826DNase-
116826caseinase-3.4.21.50
116826catalase+1.11.1.6
116826tween esterase-
116826gamma-glutamyltransferase+2.3.2.2
116826lecithinase+
116826lipase-
116826lysine decarboxylase-4.1.1.18
116826ornithine decarboxylase-4.1.1.17
116826phenylalanine ammonia-lyase-4.3.1.24
116826protease+
116826tryptophan deaminase-
116826urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382trypsin-3.4.21.4
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18467+-+-+++-+++---+-+--
116826+++-++---++---+-----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18467-+--+-+--++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116826+++----------------+-----------+---------------------------++-----------+-----------+----+++-++----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
9228soilChichibu districtJapanJPNAsia
67770SoilChichibu, Saitama Pref.JapanJPNAsia
116826Environment, SoilChichibuJapanJPNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
92281Risk group (German classification)
184671German classification
1168261Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces blastmyceticus gene for 16S rRNA, partial sequenceAB122734564ena68180
20218Streptomyces blastmyceticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4184D44025120ena68180
20218Streptomyces blastmyceticus gene for 16S rRNA, partial sequence, strain: NBRC 12747AB2499101449ena68180
20218Streptomyces blastmyceticus strain NRRL B-5480 16S ribosomal RNA gene, partial sequenceAY9998021492ena68180

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces blastmyceticus NBRC 127472582581649draftimg68180
66792Streptomyces blastmyceticus NBRC 12747.2582581650draftimg68180

External links

@ref: 9228

culture collection no.: DSM 40029, ATCC 19731, CBS 470.68, ETH 31571, IFO 12747, ISP 5029, NBRC 12747, RIA 1012, JCM 4184, BCRC 13387, CECT 3257, CGMCC 4.1647, CIP 108151, JCM 4565, NCB 77, NCIMB 9800, NRRL B-5480, VKM Ac-766, CCRC 13387, KCC S-0565, AS 4.1647

straininfo link

  • @ref: 84158
  • straininfo: 37090

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny25030519Streptomyces zagrosensis sp. nov., isolated from soil.Mohammadipanah F, Hamedi J, Sproer C, Rohde M, Montero-Calasanz MDC, Klenk HPInt J Syst Evol Microbiol10.1099/ijs.0.064527-02014Bacterial Typing Techniques, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Iran, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purificationGenetics
Metabolism29904063Biosynthetic studies on teleocidins in Streptomyces.Abe IJ Antibiot (Tokyo)10.1038/s41429-018-0069-42018Crystallography, X-Ray, Dimethylallyltranstransferase/genetics, Enzyme Activation/drug effects, Lyngbya Toxins/*biosynthesis/*genetics/metabolism, Methyltransferases/genetics, Mixed Function Oxygenases/genetics, Peptide Synthases/genetics, Protein Kinase C/metabolism, Streptomyces/*genetics/*metabolism, Terpenes/chemistryPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9228Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40029)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40029
18467Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40029.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38176Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5742
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
84158Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID37090.1StrainInfo: A central database for resolving microbial strain identifiers
116826Curators of the CIPCollection of Institut Pasteur (CIP 108151)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108151