Strain identifier

BacDive ID: 15043

Type strain: No

Species: Streptomyces bacillaris

Strain Designation: WC3068

Strain history: CIP <- 2004, CCUG, Streptomyces griseobrunneus <- 1981, M. Ridell, Univ. of Göteborg, Göteborg, Sweden <- S.T. Williams, Liverpool, UK

NCBI tax ID(s): 66894 (species)

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General

@ref: 9162

BacDive-ID: 15043

DSM-Number: 40066

keywords: 16S sequence, Bacteria, facultative anaerobe, Gram-positive, rod-shaped

description: Streptomyces bacillaris WC3068 is a facultative anaerobe, Gram-positive, rod-shaped bacterium that was isolated from sewage.

NCBI tax id

  • NCBI tax id: 66894
  • Matching level: species

strain history

@refhistory
9162<- E.B. Shirling, ISP <- S.A. Waksman, IMRU
67770KCC S-0380 <-- IFO 12775 <-- SAJ <-- ISP 5066 <-- IMRU 3068.
116696CIP <- 2004, CCUG, Streptomyces griseobrunneus <- 1981, M. Ridell, Univ. of Göteborg, Göteborg, Sweden <- S.T. Williams, Liverpool, UK

doi: 10.13145/bacdive15043.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces bacillaris
  • full scientific name: Streptomyces bacillaris (Krassilnikov 1958) Pridham 1970 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Actinomyces bacillaris
    20215Streptomyces griseobrunneus

@ref: 9162

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces bacillaris

full scientific name: Streptomyces bacillaris (Krassilnikov 1958) Pridham 1970

strain designation: WC3068

type strain: no

Morphology

cell morphology

  • @ref: 116696
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 116696
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9162GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
38261MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
116696CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperature
18478positiveoptimum28
9162positivegrowth28
38261positivegrowth30
67770positivegrowth28
116696positivegrowth25-41
116696nogrowth10
116696nogrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 116696
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
116696NaClpositivegrowth0-4 %
116696NaClnogrowth6 %
116696NaClnogrowth8 %
116696NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1847817234glucose+
1847822599arabinose-
1847817992sucrose-
1847818222xylose-
1847817268myo-inositol-
1847829864mannitol+
1847828757fructose+
1847826546rhamnose-
1847816634raffinose-
1847862968cellulose+
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis
11669616947citrate-carbon source
1166964853esculin+hydrolysis
116696606565hippurate+hydrolysis
11669617632nitrate+reduction
11669616301nitrite-reduction
11669635020tributyrin+hydrolysis
11669617632nitrate+respiration

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
11669635581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
11669615688acetoin-
11669617234glucose-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
116696oxidase-
116696beta-galactosidase+3.2.1.23
116696alcohol dehydrogenase-1.1.1.1
116696gelatinase+
116696amylase+
116696DNase+
116696caseinase+3.4.21.50
116696catalase+1.11.1.6
116696tween esterase-
116696gamma-glutamyltransferase+2.3.2.2
116696lecithinase-
116696lipase-
116696lysine decarboxylase-4.1.1.18
116696ornithine decarboxylase-4.1.1.17
116696phenylalanine ammonia-lyase-4.3.1.24
116696tryptophan deaminase-
116696urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382alpha-mannosidase-3.2.1.24

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18478+++++++++++-+-+++-+
116696++--++-++-+---++----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18478+++++-+--++

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116696----+/------+/-----------+/---+/-----------+/-+/-------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116696+++++----+++---+++++-+----+----+-+---------------++--+-----+++----------++---+-++---+-+-++++-++-+--

Isolation, sampling and environmental information

isolation

@refsample type
9162sewage
116696Environment, Sewage

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Wastewater

taxonmaps

  • @ref: 69479
  • File name: preview.99_210.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_102;97_109;98_120;99_210&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: AY999888
  • Sequence Identity:
  • Total samples: 112
  • soil counts: 103
  • aquatic counts: 1
  • animal counts: 6
  • plant counts: 2

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
91621Risk group (German classification)
184781German classification
1166961Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces griseobrunneus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4380D44095120nuccore66894
20218Streptomyces griseobrunneus strain DSM 40066 16S ribosomal RNA gene, partial sequenceGU383167781nuccore66894
9162Streptomyces griseobrunneus gene for 16S rRNA, partial sequence, strain: NBRC 12775AB2499121453nuccore66894
67770Streptomyces griseobrunneus strain AS 4.1838 16S ribosomal RNA gene, partial sequenceAY9998881437nuccore66894

GC content

  • @ref: 67770
  • GC-content: 72
  • method: fluorimetric

External links

@ref: 9162

culture collection no.: DSM 40066, ATCC 19762, CBS 500.68, CCUG 11105, ETH 31437, ETH 31581, IFO 12775, IMET 42052, IMRU 3068, ISP 5066, JCM 4380, KCC S-0380, NBRC 12775, NRRL B-2095, RIA 1042, Waksman 218, BCRC 13674, CGMCC 4.1838, HAMBI 1015, KCCM 40612, NCIMB 12975, VKM Ac-753, VTT E-072672, CIP 108231, CBS 498.68

straininfo link

  • @ref: 84149
  • straininfo: 362270

literature

  • topic: Enzymology
  • Pubmed-ID: 3714878
  • title: Construction and characterization of new cloning vectors derived from Streptomyces griseobrunneus plasmid pBT1 and containing amikacin and sulfomycin resistance genes.
  • authors: Nakanishi N, Oshida T, Yano S, Takeda K, Yamaguchi T, Ito Y
  • journal: Plasmid
  • DOI: 10.1016/0147-619x(86)90040-5
  • year: 1986
  • mesh: Amikacin/biosynthesis/*pharmacology, *Anti-Bacterial Agents, Cloning, Molecular, DNA, Bacterial/analysis, Drug Resistance, Microbial, Genes, Bacterial, *Genetic Vectors, Kanamycin/*analogs & derivatives, Nucleic Acid Hybridization, Peptide Biosynthesis, Peptides/pharmacology, *Plasmids, Streptomyces/drug effects/*genetics/metabolism, Thiostrepton/pharmacology
  • topic2: Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9162Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40066)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40066
18478Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40066.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38261Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5831
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
84149Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID362270.1StrainInfo: A central database for resolving microbial strain identifiers
116696Curators of the CIPCollection of Institut Pasteur (CIP 108231)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108231