Strain identifier

BacDive ID: 14909

Type strain: No

Species: Streptomyces albidoflavus

Strain Designation: AZ-2

Strain history: NRRL B-2593 <-- INA 13195/54.

NCBI tax ID(s): 1886 (species)

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General

@ref: 9818

BacDive-ID: 14909

DSM-Number: 40814

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, Gram-positive

description: Streptomyces albidoflavus AZ-2 is a spore-forming, Gram-positive bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 1886
  • Matching level: species

strain history

@refhistory
9818<- H.D. Tresner, AZ-2
67770NRRL B-2593 <-- INA 13195/54.

doi: 10.13145/bacdive14909.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces albidoflavus
  • full scientific name: Streptomyces albidoflavus (Rossi Doria 1891) Waksman and Henrici 1948 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Streptomyces griseus subsp. solvifaciens
    20215Streptomyces limosus
    20215Actinomyces sampsonii
    20215Actinomyces globisporus subsp. caucasicus
    20215Cladothrix odorifera
    20215Streptomyces felleus
    20215Streptomyces sampsonii
    20215Streptomyces odorifer
    20215Streptomyces coelicolor
    20215Streptothrix coelicolor
    20215Streptotrix albidoflava
    20215Streptomyces globisporus subsp. caucasicus
    20215Streptomyces canescens
    20215Streptomyces champavatii

@ref: 9818

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces albidoflavus

full scientific name: Streptomyces albidoflavus (Rossi Doria 1891) Waksman and Henrici 1948 emend. Nouioui et al. 2018

strain designation: AZ-2

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no91.5
6948091.329positive

colony morphology

@refcolony colorincubation periodmedium used
19816Brown beige10-14 daysISP 2
19816Brown beige10-14 daysISP 3
19816Brown beige10-14 daysISP 4
19816Saffron yellow10-14 daysISP 5
19816Brown beige10-14 daysISP 6
19816Sand yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19816yesAerial MyceliumCream (9001)ISP 2
19816yesAerial MyceliumCream (9001)ISP 3
19816yesAerial MyceliumCream (9001)ISP 4
19816yesAerial MyceliumCream (9001)ISP 5
19816yesAerial MyceliumCream (9001)ISP 6
19816yesAerial MyceliumISP 7

multimedia

  • @ref: 9818
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40814.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9818GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19816ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19816ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19816ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19816ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19816ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19816ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9818KOSMOTOGA MEDIUM (DSMZ Medium 284)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium284.pdf

culture temp

@refgrowthtypetemperature
19816positiveoptimum28
9818positivegrowth28
67770positivegrowth28

Physiology and metabolism

spore formation

@refspore formationconfidence
69480yes94.584
69481yes100

metabolite utilization

@refChebi-IDmetaboliteutilization activity
1981662968cellulose+
1981616634raffinose-
1981626546rhamnose-
1981628757fructose+
1981629864mannitol+
1981617268myo-inositol-
1981618222xylose-
1981617992sucrose+
1981622599arabinose-
1981617234glucose+

enzymes

@refvalueactivityec
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19816+++-++--+++-+-+++--

Isolation, sampling and environmental information

isolation

  • @ref: 9818
  • sample type: soil
  • country: USSR
  • continent: Asia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_720.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_91;97_287;98_384;99_720&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: AB249937
  • Sequence Identity:
  • Total samples: 129
  • soil counts: 39
  • aquatic counts: 5
  • animal counts: 85

Safety information

risk assessment

  • @ref: 9818
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
9818Streptomyces globisporus subsp. caucasicus strain NRRL B-2593 16S ribosomal RNA gene, partial sequenceEF1786761419nuccore371721
67770Streptomyces albidoflavus NBRC 100770 gene for 16S rRNA, partial sequenceAB2499371482nuccore1886
124043Streptomyces albidoflavus strain NBRC 100770 16S ribosomal RNA gene, partial sequence.OQ1952531478nuccore1886

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces albidoflavus strain NBRC 1007701886.38wgspatric1886
67770Streptomyces albidoflavus NBRC 100770GCA_004195745contigncbi1886

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes91.329no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no97.894no
69480spore-formingspore-formingAbility to form endo- or exosporesyes94.584no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes88.51no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.5yes
69480flagellatedmotile2+Ability to perform flagellated movementno91.5no

External links

@ref: 9818

culture collection no.: DSM 40814, ATCC 19907, CBS 120.60, INA 13195/54, NRRL B-2593, JCM 9867, CGMCC 4.1968, ISP 5157, NBRC 100770, RIA 319, VKM Ac-1846

straininfo link

  • @ref: 84018
  • straininfo: 37154

Reference

@idauthorscataloguedoi/urltitle
9818Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40814)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40814
19816Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40814.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84018Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID37154.1StrainInfo: A central database for resolving microbial strain identifiers
124043Dr. Isabel Schober, Dr. Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy