Strain identifier

BacDive ID: 14861

Type strain: Yes

Species: Kitasatospora gansuensis

Strain Designation: 2050-015

Strain history: I. Groth 2050-015 (=HKI 0314) <-- Ch. Weigel.

NCBI tax ID(s): 258050 (species)

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General

@ref: 12090

BacDive-ID: 14861

DSM-Number: 44786

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Kitasatospora gansuensis 2050-015 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from forest soil.

NCBI tax id

  • NCBI tax id: 258050
  • Matching level: species

strain history

@refhistory
12090<- I. Groth, HKI; 2050-015 <- Ch. Weigel
67770I. Groth 2050-015 (=HKI 0314) <-- Ch. Weigel.

doi: 10.13145/bacdive14861.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Kitasatospora
  • species: Kitasatospora gansuensis
  • full scientific name: Kitasatospora gansuensis Groth et al. 2004

@ref: 12090

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Kitasatospora

species: Kitasatospora gansuensis

full scientific name: Kitasatospora gansuensis Groth et al. 2004

strain designation: 2050-015

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
31218positiveno
69480positive100

colony morphology

@refcolony colorincubation periodmedium used
19704Beige10-14 daysISP 2
19704Beige10-14 daysISP 3
19704Beige10-14 daysISP 4
19704Beige10-14 daysISP 5
19704Golden yellow10-14 daysISP 6
19704Beige10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19704yesAerial MyceliumCreamISP 2
19704yesAerial MyceliumCreamISP 3
19704yesAerial MyceliumCreamISP 4
19704yesAerial MyceliumCreamISP 5
19704noISP 6
19704yesAerial MyceliumCreamISP 7

pigmentation

  • @ref: 31218
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12090ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
19704ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19704ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19704ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19704ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19704ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19704ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
12090GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
12090STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
12090positivegrowth28mesophilic
19704positiveoptimum28mesophilic
31218positivegrowth06-32
31218positiveoptimum26.5mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
31218positivegrowth5-9.5alkaliphile
31218positiveoptimum7.25

Physiology and metabolism

oxygen tolerance

  • @ref: 31218
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
31218yes
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentration
31218NaClpositivegrowth0-2.5 %
31218NaClpositiveoptimum1.25 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1970417234glucose+
1970422599arabinose+
1970417992sucrose+
1970418222xylose-
1970417268myo-inositol-
1970429864mannitol-
1970428757fructose+
1970426546rhamnose-
1970416634raffinose-
1970462968cellulose-
3121822599arabinose+carbon source
3121828757fructose+carbon source
3121817234glucose+carbon source
3121817992sucrose+carbon source
3121818222xylose+carbon source
3121817632nitrate+reduction

metabolite production

  • @ref: 31218
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

enzymes

@refvalueactivityec
31218acid phosphatase+3.1.3.2
31218alkaline phosphatase+3.1.3.1
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19704+++-+--+-++-+--+---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
12090forest soilLianhua Shan reservat, prov. GansuChinaCHNAsia
67770Soil in Lianhua Shan ReservationGansu ProvinceChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_4885.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_148;97_159;98_177;99_4885&stattab=map
  • Last taxonomy: Kitasatospora
  • 16S sequence: AY442265
  • Sequence Identity:
  • Total samples: 828
  • soil counts: 781
  • aquatic counts: 9
  • animal counts: 33
  • plant counts: 5

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
120901Risk group (German classification)
197041Risk group (German classification)

Sequence information

16S sequences

  • @ref: 12090
  • description: Kitasatospora gansuensis strain HKI 0314 16S ribosomal RNA gene, partial sequence
  • accession: AY442265
  • length: 1429
  • database: ena
  • NCBI tax ID: 258050

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Kitasatospora gansuensis DSM 44786GCA_014203705contigncbi258050
66792Kitasatospora gansuensis strain DSM 44786258050.3wgspatric258050
66792Kitasatospora gansuensis DSM 447862863349733draftimg258050

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno93.146yes
gram-positiveyes92.035no
anaerobicno99.602no
aerobicyes91.826yes
halophileno93.164yes
spore-formingyes93.269no
glucose-utilyes90.809yes
flagellatedno97.376yes
thermophileno99.288yes
glucose-fermentno88.809no

External links

@ref: 12090

culture collection no.: DSM 44786, HKI 0314, NCIMB 13974, JCM 13003, IAM 15269, NBRC 101835

straininfo link

  • @ref: 83974
  • straininfo: 134432

literature

  • topic: Phylogeny
  • Pubmed-ID: 15545445
  • title: Five novel Kitasatospora species from soil: Kitasatospora arboriphila sp. nov., K. gansuensis sp. nov., K. nipponensis sp. nov., K. paranensis sp. nov. and K. terrestris sp. nov.
  • authors: Groth I, Rodriguez C, Schutze B, Schmitz P, Leistner E, Goodfellow M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63070-0
  • year: 2004
  • mesh: Anti-Bacterial Agents/pharmacology, Bacterial Typing Techniques, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis/isolation & purification, Genes, rRNA/genetics, Hydrogen-Ion Concentration, Japan, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Saline Solution, Hypertonic, Sequence Analysis, DNA, *Soil Microbiology, Streptomycetaceae/*classification/cytology/*isolation & purification/physiology, Temperature
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
12090Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44786)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44786
19704Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44786.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31218Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2754428776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83974Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID134432.1StrainInfo: A central database for resolving microbial strain identifiers