Strain identifier

BacDive ID: 14815

Type strain: Yes

Species: Streptococcus orisratti

Strain Designation: A63

Strain history: CIP <- 2001, H. Zhu, New South Wales Univ., Sydney, Australia: strain A63

NCBI tax ID(s): 1123311 (strain), 114652 (species)

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General

@ref: 5935

BacDive-ID: 14815

DSM-Number: 15617

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic

description: Streptococcus orisratti A63 is a microaerophile, mesophilic bacterium that was isolated from tooth surface of laboratory rat.

NCBI tax id

NCBI tax idMatching level
1123311strain
114652species

strain history

@refhistory
5935<- ATCC <- H. Zhu; A63
386602001, H. Zhu, Univ. New South Wales, Sydney, Australia: strain A63
121446CIP <- 2001, H. Zhu, New South Wales Univ., Sydney, Australia: strain A63

doi: 10.13145/bacdive14815.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus orisratti
  • full scientific name: Streptococcus orisratti Zhu et al. 2000

@ref: 5935

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus orisratti

full scientific name: Streptococcus orisratti Zhu et al. 2000

strain designation: A63

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no94.795
69480100positive
121446nopositivecoccus-shaped

colony morphology

  • @ref: 121446
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5935COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
5935BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
38660MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
121446CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
5935positivegrowth37mesophilic
38660positivegrowth37mesophilic
55949positivegrowth37mesophilic
121446positivegrowth25-45
121446nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5935microaerophile
55949microaerophile
121446facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.995

halophily

  • @ref: 121446
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin+builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
121446esculin+hydrolysis4853
121446hippurate+hydrolysis606565
121446nitrate-reduction17632
121446nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose+builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen+builds acid from28087
68381pullulan+builds acid from27941
68381maltose+builds acid from17306
68381melibiose+builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin-
12144615688acetoin-

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
121446oxidase-
121446beta-galactosidase-3.2.1.23
121446alcohol dehydrogenase-1.1.1.1
121446catalase-1.11.1.6
121446gamma-glutamyltransferase-2.3.2.2
121446lysine decarboxylase-4.1.1.18
121446ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121446--++-+----+++--+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121446---------++++--------+/-+++++++++++-+++-+---------+

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
5935-+--+----++++----+-----++++-+---

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinentisolation date
5935tooth surface of laboratory rat
55949Rat,tooth surface of laboratory rat1996
121446Animal, Rat, tooth surfaceSydney, New South WalesAustraliaAUSAustralia and Oceania1994

isolation source categories

Cat1Cat2Cat3
#Engineered#Laboratory
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body-Site#Oral cavity and airways#Tooth

taxonmaps

  • @ref: 69479
  • File name: preview.99_33017.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_3165;97_19545;98_24421;99_33017&stattab=map
  • Last taxonomy: Streptococcus
  • 16S sequence: AF124350
  • Sequence Identity:
  • Total samples: 255
  • soil counts: 4
  • aquatic counts: 8
  • animal counts: 243

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
59351Risk group (German classification)
1214462Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5935
  • description: Streptococcus orisratti 16S ribosomal RNA gene, partial sequence
  • accession: AF124350
  • length: 1337
  • database: ena
  • NCBI tax ID: 114652

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus orisratti DSM 15617GCA_000380105scaffoldncbi1123311
66792Streptococcus orisratti DSM 156171123311.3wgspatric1123311
66792Streptococcus orisratti DSM 156172515154108draftimg1123311

GC content

  • @ref: 5935
  • GC-content: 39.6-43.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno94.326no
flagellatedno95.682no
gram-positiveyes94.144no
anaerobicno92.686yes
aerobicno98.012yes
halophileyes77.728no
spore-formingno95.428no
thermophileno99.687yes
glucose-utilyes88.409no
glucose-fermentyes81.536no

External links

@ref: 5935

culture collection no.: DSM 15617, ATCC 700640, CCUG 43577, CIP 106965

straininfo link

  • @ref: 83932
  • straininfo: 44051

literature

  • topic: Phylogeny
  • Pubmed-ID: 10826787
  • title: A new species of oral Streptococcus isolated from Sprague-Dawley rats, Streptococcus orisratti sp. nov.
  • authors: Zhu H, Willcox MD, Knox KW
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-50-1-55
  • year: 2000
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/analysis/genetics, Fermentation, Molar/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, RNA, Ribosomal, 16S/genetics, Rats, Rats, Sprague-Dawley/*microbiology, Sequence Analysis, DNA, Streptococcus/*classification/isolation & purification/metabolism
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
5935Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15617)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15617
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
38660Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19153
55949Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 43577)https://www.ccug.se/strain?id=43577
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83932Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44051.1StrainInfo: A central database for resolving microbial strain identifiers
121446Curators of the CIPCollection of Institut Pasteur (CIP 106965)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106965