Strain identifier

BacDive ID: 14810

Type strain: Yes

Species: Streptococcus sinensis

Strain Designation: HKU4, HKU 4

Strain history: CIP <- 2002, K.Y. Yuen, Hong Kong Univ., Hong Kong, China: strain HKU 4

NCBI tax ID(s): 176090 (species)

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General

@ref: 5657

BacDive-ID: 14810

DSM-Number: 14990

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, coccus-shaped

description: Streptococcus sinensis HKU4 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from infective endocarditis.

NCBI tax id

  • NCBI tax id: 176090
  • Matching level: species

strain history

@refhistory
5657<- K.-Y. Yuen; HKU4
122629CIP <- 2002, K.Y. Yuen, Hong Kong Univ., Hong Kong, China: strain HKU 4

doi: 10.13145/bacdive14810.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus sinensis
  • full scientific name: Streptococcus sinensis Woo et al. 2002

@ref: 5657

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus sinensis

full scientific name: Streptococcus sinensis Woo et al. 2002

strain designation: HKU4, HKU 4

type strain: yes

Morphology

cell morphology

  • @ref: 122629
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 122629
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5657TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
32868MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
122629CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
5657positivegrowth37mesophilic
32868positivegrowth37mesophilic
122629positivegrowth25-41
122629nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122629
  • oxygen tolerance: facultative anaerobe

halophily

  • @ref: 122629
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
122629esculin+hydrolysis4853
122629hippurate+hydrolysis606565
122629nitrate-reduction17632
122629nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose-builds acid from17716
68381trehalose+builds acid from27082
68381raffinose-builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin-
12262915688acetoin-

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
122629oxidase-
122629beta-galactosidase+3.2.1.23
122629alcohol dehydrogenase-1.1.1.1
122629catalase-1.11.1.6
122629gamma-glutamyltransferase-2.3.2.2
122629lysine decarboxylase-4.1.1.18
122629ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122629--++-++----+-+-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122629---------++++--------++++++++-++---+--+---------+/-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
5657-+--------+-+----+------+/-+--+---

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
5657infective endocarditisHong KongHKGAsia
122629Human, BloodChinaCHNAsiaHong Kong

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host Body-Site#Organ#Heart

taxonmaps

  • @ref: 69479
  • File name: preview.99_698.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_4;97_490;98_561;99_698&stattab=map
  • Last taxonomy: Streptococcus sinensis
  • 16S sequence: AF432856
  • Sequence Identity:
  • Total samples: 25117
  • soil counts: 111
  • aquatic counts: 266
  • animal counts: 24664
  • plant counts: 76

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
56572Risk group (German classification)
1226292Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5657
  • description: Streptococcus sinensis strain HKU4 16S ribosomal RNA, partial sequence
  • accession: AF432856
  • length: 1512
  • database: ena
  • NCBI tax ID: 176090

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus sinensis HKU4GCA_000767835contigncbi176090
66792Streptococcus sinensis HKU4176090.4wgspatric176090
66792Streptococcus sinensis HKU42636415646draftimg176090

GC content

  • @ref: 5657
  • GC-content: 53

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno96.736no
gram-positiveyes94.237no
anaerobicno94.001no
aerobicno97.631no
halophileyes77.201no
spore-formingno92.403no
thermophileno99.326yes
glucose-utilyes86.868no
flagellatedno97.001no
glucose-fermentyes88.474no

External links

@ref: 5657

culture collection no.: DSM 14990, LMG 21517, CIP 107480

straininfo link

  • @ref: 83927
  • straininfo: 93894

Reference

@idauthorscataloguedoi/urltitle
5657Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14990)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14990
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32868Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4920
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83927Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID93894.1StrainInfo: A central database for resolving microbial strain identifiers
122629Curators of the CIPCollection of Institut Pasteur (CIP 107480)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107480