Strain identifier

BacDive ID: 14804

Type strain: Yes

Species: Streptococcus thoraltensis

Strain Designation: S69

Strain history: CIP <- 1998, LMG <- L. Devriese, Ghent Univ., Ghent, Belgium: strain S69

NCBI tax ID(s): 1123318 (strain), 55085 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 4646

BacDive-ID: 14804

DSM-Number: 12221

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic

description: Streptococcus thoraltensis S69 is a microaerophile, mesophilic bacterium that was isolated from pig intestine.

NCBI tax id

NCBI tax idMatching level
55085species
1123318strain

strain history

@refhistory
4646<- LMG <- Devriese, S69
123533CIP <- 1998, LMG <- L. Devriese, Ghent Univ., Ghent, Belgium: strain S69

doi: 10.13145/bacdive14804.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus thoraltensis
  • full scientific name: Streptococcus thoraltensis Devriese et al. 1997

@ref: 4646

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus thoraltensis

full scientific name: Streptococcus thoraltensis Devriese et al. 1997

strain designation: S69

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.597
6948099.999positive
123533nopositivecoccus-shaped

colony morphology

@refincubation period
46461-2 days
123533

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4646COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
4646TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
39308MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
123533CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
4646positivegrowth37mesophilic
39308positivegrowth37mesophilic
123533positivegrowth25-41
123533nogrowth10psychrophilic
123533nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
4646microaerophile
123533facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.992

halophily

  • @ref: 123533
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

murein

  • @ref: 4646
  • murein short key: A11.20
  • type: A3alpha L-Lys-L-Ser-L-Ala2

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol+builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin+builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
123533esculin+hydrolysis4853
123533hippurate+hydrolysis606565
123533nitrate-reduction17632
123533nitrite-reduction16301
68381arginine+hydrolysis29016
68381D-ribose+builds acid from16988
68381D-mannitol+builds acid from16899
68381sorbitol+builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose+builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose+builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate+hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan+builds acid from27941
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
12353315688acetoin+

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase+3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
123533oxidase-
123533beta-galactosidase+3.2.1.23
123533alcohol dehydrogenase-1.1.1.1
123533catalase-1.11.1.6
123533gamma-glutamyltransferase-2.3.2.2
123533lysine decarboxylase-4.1.1.18
123533ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123533-+---+----++--++----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123533---+++---++++----++-+/-++++++++++++-+++/--++-+--+----

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
4646++-++-++++++++--++----+-++--+---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBE
123533----------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
4646pig intestineBelgiumBelgiumBELEurope
123533Animal, Pig, intestineBelgiumBELEurope1984

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Suidae (Pig,Swine)
#Host Body-Site#Gastrointestinal tract

taxonmaps

  • @ref: 69479
  • File name: preview.99_6873.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_359;97_2290;98_5059;99_6873&stattab=map
  • Last taxonomy: Streptococcus thoraltensis subclade
  • 16S sequence: Y09007
  • Sequence Identity:
  • Total samples: 3375
  • soil counts: 68
  • aquatic counts: 79
  • animal counts: 3179
  • plant counts: 49

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
46461Risk group (German classification)
1235332Risk group (French classification)

Sequence information

16S sequences

  • @ref: 4646
  • description: S.thoraltensis 16S rRNA gene
  • accession: Y09007
  • length: 1519
  • database: ena
  • NCBI tax ID: 55085

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus thoraltensis DSM 12221GCA_000380145scaffoldncbi1123318
66792Streptococcus thoraltensis DSM 122211123318.3wgspatric1123318
66792Streptococcus thoraltensis DSM 122212515154195draftimg1123318

GC content

  • @ref: 4646
  • GC-content: 40
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno95.972no
flagellatedno97.886no
gram-positiveyes92.948no
anaerobicno97.506yes
aerobicno96.224yes
halophileyes91.126no
spore-formingno96.22no
thermophileno99.576yes
glucose-utilyes91.331no
glucose-fermentyes88.51no

External links

@ref: 4646

culture collection no.: DSM 12221, LMG 13593, CIP 105518

straininfo link

  • @ref: 83921
  • straininfo: 10165

literature

  • topic: Phylogeny
  • Pubmed-ID: 9336908
  • title: Streptococcus hyovaginalis sp. nov. and Streptococcus thoraltensis sp. nov., from the genital tract of sows.
  • authors: Devriese LA, Pot B, Vandamme P, Kersters K, Collins MD, Alvarez N, Haesebrouck F, Hommez J
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-47-4-1073
  • year: 1997
  • mesh: Animals, Bacterial Proteins/analysis, DNA, Bacterial/*analysis, Electrophoresis, Polyacrylamide Gel, Female, Genitalia, Female/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/*analysis, Streptococcal Infections/*veterinary, Streptococcus/chemistry/*classification/*genetics/growth & development/physiology, Swine, Swine Diseases
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitle
4646Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12221)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12221
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39308Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17547
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83921Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID10165.1StrainInfo: A central database for resolving microbial strain identifiers
123533Curators of the CIPCollection of Institut Pasteur (CIP 105518)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105518