Strain identifier

BacDive ID: 14795

Type strain: Yes

Species: Streptococcus cristatus

Strain Designation: CR311

Strain history: CIP <- 1999, ATCC <- A.L. Cockendall <- P. Handley: strain CR 311, Streptococcus suis

NCBI tax ID(s): 889201 (strain), 45634 (species)

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General

@ref: 3218

BacDive-ID: 14795

DSM-Number: 8249

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, Gram-positive, coccus-shaped, human pathogen

description: Streptococcus cristatus CR311 is a microaerophile, Gram-positive, coccus-shaped human pathogen that was isolated from periodontal abscess.

NCBI tax id

NCBI tax idMatching level
45634species
889201strain

strain history

@refhistory
3218<- NCTC <- P.Handley, CR311 (Streptococcus sanguis)
67770LMG 16320 <-- CCUG 33481 <-- NCTC 12479 <-- P. Handley; Manchester Univ., UK; CR311 ("Streptococcus sanguis").
118788CIP <- 1999, ATCC <- A.L. Cockendall <- P. Handley: strain CR 311, Streptococcus suis

doi: 10.13145/bacdive14795.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus cristatus
  • full scientific name: Streptococcus cristatus corrig. Handley et al. 1991

  • synonyms

    @refsynonym
    20215Streptococcus crista
    20215Streptococcus oligofermentans

@ref: 3218

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus cristatus

full scientific name: Streptococcus cristatus Handley et al. 1991

strain designation: CR311

type strain: yes

Morphology

cell morphology

  • @ref: 118788
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@refincubation periodhemolysis ability
32182-3 days
1187881

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3218COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
3218TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
39407MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
118788CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperature
3218positivegrowth37
39407positivegrowth37
67770positivegrowth37
118788positivegrowth25-45
118788nogrowth10

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
3218microaerophile
118788facultative anaerobe

spore formation

  • @ref: 69481
  • spore formation: no
  • confidence: 100

halophily

  • @ref: 118788
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

murein

  • @ref: 3218
  • murein short key: A11.05
  • type: A3alpha L-Lys-L-Ala2

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
118788esculin-hydrolysis4853
118788hippurate+hydrolysis606565
118788nitrate-reduction17632
118788nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose-builds acid from17716
68381trehalose+builds acid from27082
68381raffinose-builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside-builds acid from320055
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin-
11878815688acetoin-

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6
118788oxidase-
118788beta-galactosidase+3.2.1.23
118788alcohol dehydrogenase-1.1.1.1
118788catalase-1.11.1.6
118788gamma-glutamyltransferase-2.3.2.2
118788lysine decarboxylase-4.1.1.18
118788ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118788--+++++----+--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
118788+--------++++--------+-++++++/--++-------+-+/-+/-------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
3218--+-------+-+----+---++--+---+/---
3218----------+-+----+---++/---+------
3218--+-------+-+----+---+---+---+--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
3218periodontal abscessUnited KingdomGBREurope
51543Human periodontal abscess
67770Human oral cavity
118788Human, Periodontal abscess

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Oral cavity and airways
#Host Body-Site#Other#Abscess

taxonmaps

  • @ref: 69479
  • File name: preview.99_2187.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_4;97_490;98_1706;99_2187&stattab=map
  • Last taxonomy: Streptococcus cristatus subclade
  • 16S sequence: EU156757
  • Sequence Identity:
  • Total samples: 185321
  • soil counts: 3388
  • aquatic counts: 5410
  • animal counts: 175025
  • plant counts: 1498

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
3218yes, in single cases1Risk group (German classification)
1187882Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptococcus cristatus strain ATCC 51100 16S ribosomal RNA gene, partial sequenceAY5844761469nuccore889201
20218Streptococcus cristatus strain ATCC 51100 16S ribosomal RNA gene, partial sequenceEU1567571329nuccore889201
20218Streptococcus cristatus ATCC 51100 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceJN181382517nuccore889201
20218Streptococcus cristatus strain CCUG 33481 16S-23S ribosomal RNA intergenic spacer, partial sequenceDQ204522252nuccore889201
3218Streptococcus cristatus strain ATCC 51100 16S ribosomal RNA gene, complete sequenceAY1883471533nuccore889201
124043Streptococcus cristatus gene for 16S rRNA, partial sequence.AB0083131403nuccore889201
124043Streptococcus cristatus ATCC 51100 gene for 16S rRNA, partial sequence.LC752409588nuccore889201
124043Streptococcus cristatus ATCC 51100 gene for 16S rRNA, partial sequence.LC752417588nuccore889201

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus cristatus ATCC 51100 NCTC 12479GCA_900475445completencbi889201
66792Streptococcus cristatus ATCC 51100GCA_011612585completencbi889201
66792Streptococcus cristatus ATCC 51100GCA_000222765contigncbi889201
66792Streptococcus cristatus ATCC 51100GCA_000187855scaffoldncbi889201
66792Streptococcus cristatus ATCC 51100889201.3wgspatric889201
66792Streptococcus cristatus ATCC 51100889201.21completepatric889201
66792Streptococcus cristatus ATCC 51100889201.11wgspatric889201
66792Streptococcus cristatus ATCC 51100 strain NCTC12479889201.20completepatric889201
66792Streptococcus cristatus ATCC 51100649990004draftimg889201

GC content

@refGC-contentmethod
321843.0
6777042.6thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes86.129no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no92.435no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no93.407no
69480spore-formingspore-formingAbility to form endo- or exosporesno83.103no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.5yes
69480flagellatedmotile2+Ability to perform flagellated movementno89.5no

External links

@ref: 3218

culture collection no.: DSM 8249, ATCC 51100, NCTC 12479, CCUG 33481, LMG 16320, JCM 31122, CIP 105954

straininfo link

  • @ref: 83912
  • straininfo: 8020

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1742199Streptococcus crista sp. nov., a viridans streptococcus with tufted fibrils, isolated from the human oral cavity and throat.Handley P, Coykendall A, Beighton D, Hardie JM, Whiley RAInt J Syst Bacteriol10.1099/00207713-41-4-5431991DNA, Bacterial, Humans, Mouth/*microbiology, Pharynx/*microbiology, Phenotype, Sequence Homology, Nucleic Acid, Streptococcus/classification/genetics/*isolation & purification/ultrastructurePathogenicity
Pathogenicity15836513Association of a novel high molecular weight, serine-rich protein (SrpA) with fibril-mediated adhesion of the oral biofilm bacterium Streptococcus cristatus.Handley PS, Correia FF, Russell K, Rosan B, DiRienzo JMOral Microbiol Immunol10.1111/j.1399-302X.2004.00190.x2005Amino Acid Sequence, Bacterial Adhesion/genetics/physiology, Bacterial Proteins/*genetics/metabolism, Biofilms, Chromosome Mapping, Dental Plaque/microbiology/ultrastructure, Fimbriae, Bacterial/*genetics/ultrastructure, Humans, Molecular Sequence Data, Serine/chemistry, Species Specificity, Streptococcus/*genetics/physiology/ultrastructureGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3218Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8249)https://www.dsmz.de/collection/catalogue/details/culture/DSM-8249
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39407Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18030
51543Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33481)https://www.ccug.se/strain?id=33481
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83912Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8020.1StrainInfo: A central database for resolving microbial strain identifiers
118788Curators of the CIPCollection of Institut Pasteur (CIP 105954)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105954
124043Dr. Isabel Schober, Dr. Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy