Strain identifier

BacDive ID: 14758

Type strain: Yes

Species: Streptococcus pyogenes

Strain Designation: SF 130, T1, SF 130/13, TI

Strain history: CIP <- 1956, R. Wahl: strain A4t1, Streptococcus pyogenes <- Williams, Public Hlth. Lab., Colindale, London, UK: strain SF 130/13 <- R. Lancefield, typing strain TI <- Griffith: strain SF 130

NCBI tax ID(s): 1123316 (strain), 1314 (species)

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General

@ref: 8915

BacDive-ID: 14758

DSM-Number: 20565

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped

description: Streptococcus pyogenes SF 130 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from scarlet fever.

NCBI tax id

NCBI tax idMatching level
1123316strain
1314species

strain history

@refhistory
8915<- NCTC; NCTC 8198 <- R. E. O. Williams; <- F. Griffith; SF 130 <- R. Lancefield; T1
67770ATCC 12344 <-- R. Lancefield typing strain T1 <-- F. Griffith SF 130.
122086CIP <- 1956, R. Wahl: strain A4t1, Streptococcus pyogenes <- Williams, Public Hlth. Lab., Colindale, London, UK: strain SF 130/13 <- R. Lancefield, typing strain TI <- Griffith: strain SF 130

doi: 10.13145/bacdive14758.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus pyogenes
  • full scientific name: Streptococcus pyogenes Rosenbach 1884 (Approved Lists 1980)

@ref: 8915

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus pyogenes

full scientific name: Streptococcus pyogenes Rosenbach 1884

strain designation: SF 130, T1, SF 130/13, TI

type strain: yes

Morphology

cell morphology

  • @ref: 122086
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@reftype of hemolysishemolysis abilityincubation period
8915beta11-2 days
22952beta1
1220861

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_20565_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_20565_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_20565_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_20565_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_20565_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8915COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8915TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
39803MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
122086CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29
122086CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
8915positivegrowth37mesophilic
39803positivegrowth37mesophilic
67770positivegrowth37mesophilic
122086positivegrowth25-37mesophilic
122086nogrowth10psychrophilic
122086nogrowth41thermophilic
122086nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8915microaerophile
122086facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
22952NaClpositivegrowth6.5 %(w/v)
122086NaClpositivegrowth0-4 %
122086NaClnogrowth6 %
122086NaClnogrowth6.5 %
122086NaClnogrowth8 %
122086NaClnogrowth10 %

murein

  • @ref: 8915
  • murein short key: A11.05
  • type: A3alpha L-Lys-L-Ala2

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
229524853esculin+hydrolysis
2295217306maltose+builds acid from
2295229864mannitol-builds acid from
2295230911sorbitol-builds acid from
2295217992sucrose+builds acid from
2295227082trehalose+builds acid from
1220864853esculin+hydrolysis
122086606565hippurate+hydrolysis
12208617632nitrate-reduction
12208616301nitrite-reduction
6838129016arginine+hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
68381606565hippurate-hydrolysis
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 122086
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 122086
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
22952pyrrolidonyl arylamidase+3.4.19.3
8915catalase-1.11.1.6
8915cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase+3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase+3.5.3.6
122086oxidase-
122086beta-galactosidase+3.2.1.23
122086alcohol dehydrogenase-1.1.1.1
122086catalase-1.11.1.6
122086gamma-glutamyltransferase-2.3.2.2
122086lysine decarboxylase-4.1.1.18
122086ornithine decarboxylase-4.1.1.17
122086urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122086-+---+++--++---+----

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
8915+----+---++-+--+-+-+---+/-++--+---
8915+----+---++-+--+/--+-+----++--+---
8915+----+---++-+--+++-+---+++--+/----
8915+----+---++-+--+-+-+---+-+--+---
8915+----+---++-+----+-+----++--+---
8915+----+---++-+--+++-+---+++--+---
8915+----+---++-+----+-+---+/-++--+---
8915+----+---++-+----+-+----++--+---

Isolation, sampling and environmental information

isolation

@refsample type
8915scarlet fever
67770Scarlet fever
122086Human, Scarlet fever

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host Body-Site#Oral cavity and airways#Mouth

taxonmaps

  • @ref: 69479
  • File name: preview.99_183.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_16;97_142;98_159;99_183&stattab=map
  • Last taxonomy: Streptococcus pyogenes subclade
  • 16S sequence: LC071824
  • Sequence Identity:
  • Total samples: 6558
  • soil counts: 202
  • aquatic counts: 280
  • animal counts: 6027
  • plant counts: 49

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
89152Risk group (German classification)
1220862Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptococcus pyogenes DNA for 16S rRNA, strain ATCC 12344, NCIB 11841, MAFF 910217AB0025211393ena1314
20218Streptococcus pyogenes strain ATCC 12344 16S-23S ribosomal RNA intergenic spacer, complete sequenceAY347559350ena1314
20218Streptococcus pyogenes strain DSM 20565 16S ribosomal RNA gene, partial sequenceHQ012030935ena1314
8915Streptococcus pyogenes gene for 16S rRNA, strain:JCM 5674AB0235751501ena1314
67770Streptococcus pyogenes gene for 16S ribosomal RNA, partial sequence, strain: JCM 5674LC0718241480ena1314

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus pyogenes NCTC8198GCA_002055535completencbi1314
66792Streptococcus pyogenes CCUG 4207GCA_004028355completencbi1314
66792Streptococcus pyogenes CCUG 4207GCA_001982785scaffoldncbi1314
66792Streptococcus pyogenes DSM 20565GCA_000421785contigncbi1123316
66792Streptococcus pyogenes DSM 205651123316.3wgspatric1123316
66792Streptococcus pyogenes strain CCUG 42071314.951completepatric1314
66792Streptococcus pyogenes strain CCUG 42071314.433wgspatric1314
66792Streptococcus pyogenes strain FDAARGOS_7741314.2404completepatric1314
66792Streptococcus pyogenes strain NCTC81981314.470completepatric1314
66792Streptococcus pyogenes DSM 205652523533626draftimg1123316

GC content

  • @ref: 8915
  • GC-content: 38.0

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno98.578no
gram-positiveyes92.731no
anaerobicno97.081yes
aerobicno94.95yes
halophileyes84.574no
spore-formingno95.288no
glucose-utilyes87.641no
thermophileno99.146no
glucose-fermentyes81.275no
motileno96.195no

External links

@ref: 8915

culture collection no.: DSM 20565, ATCC 12344, NCTC 8198, JCM 5674, BCRC 14758, CCUG 12701, CCUG 4207, CECT 985, CGMCC 1.8868, CIP 56.41, NCDO 2381, NCDO 2726, NCIMB 11841, IMET 3002

straininfo link

  • @ref: 83875
  • straininfo: 13950

literature

topicPubmed-IDtitleauthorsjournalyearmeshDOItopic2
Pathogenicity60841[Studies on the mechanism of L-agglutination (author's transl)].Von Fragstein S, Wigand RZ Immunitatsforsch Immunobiol1976Antibodies, Bacterial, Arthritis, Rheumatoid/*immunology, Epitopes, Humans, Immunoglobulin G/analysis, *Latex Fixation Tests, Streptococcus pyogenes/immunology
Pathogenicity3064496Tissue cages for study of experimental streptococcal infection in rabbits. III. Influence of immunization with erythrogenic toxin type A (ET A) and its toxoid on subsequent infection with an ET A producing strain.Knoll H, Holm SE, Gerlach D, Ozegowski JH, Kohler WZentralbl Bakteriol Mikrobiol Hyg A1988Animals, Antibodies, Bacterial/biosynthesis, *Bacterial Proteins, Diffusion Chambers, Culture, Enzyme-Linked Immunosorbent Assay, Exotoxins/*immunology, Fever, Humans, Immunity, Cellular, Immunization, Lymphocyte Activation, *Membrane Proteins, Organ Size, Rabbits, Spleen/pathology, Streptococcal Infections/*immunology, Streptococcus pyogenes/*immunology, Toxoids/*immunology10.1016/s0176-6724(88)80180-9
Enzymology6442817[T-proteins of Streptococcus pyogenes. IV. Isolation of T1-protein using affinity chromatography on immobilized fibrinogen].Schmidt KH, Kohler WZentralbl Bakteriol Mikrobiol Hyg A1984Amino Acids/analysis, Bacterial Proteins/analysis/*isolation & purification, Chromatography, Affinity, Electrophoresis, Polyacrylamide Gel, Fibrinogen, Immunodiffusion, Molecular Weight, Streptococcus pyogenes/*analysis10.1016/s0176-6724(84)80021-8Phylogeny
Enzymology7013363[Studies of the heterogeneity of streptokinases; IV. Evidence for isostreptokinases in Streptococcus pyogenes type 1 (author's transl)].Gerlach D, Kohler WZentralbl Bakteriol A1981Isoelectric Point, Isoenzymes/*analysis/metabolism, Molecular Weight, Species Specificity, Streptococcus pyogenes/*enzymology, Streptokinase/*analysis/metabolismMetabolism
Pathogenicity10685108Antibacterial activity of diospyrin, isodiospyrin and bisisodiospyrin from the root of Diospyros piscatoria (Gurke) (Ebenaceae).Adeniyi BA, Fong HH, Pezzuto JM, Luyengi L, Odelola HAPhytother Res2000Anti-Bacterial Agents/isolation & purification/*pharmacology, Bacillus/drug effects, Microbial Sensitivity Tests, Mycobacterium/drug effects, Naphthoquinones/chemistry/isolation & purification/*pharmacology, Plant Extracts/isolation & purification/*pharmacology, Plant Roots/chemistry, Salmonella/drug effects, Streptococcus/drug effects10.1002/(sici)1099-1573(200003)14:2<112::aid-ptr488>3.0.co;2-tEnzymology
Phenotype12615875A multidrug efflux phenotype mutant of Streptococcus pyogenes.Jones HE, Brenwald NP, Owen KA, Gill MJJ Antimicrob Chemother2003Drug Resistance, Multiple, Bacterial/*genetics, Genes, MDR/genetics, Multidrug Resistance-Associated Proteins/genetics, *Mutation, *Phenotype, Streptococcus pyogenes/drug effects/*genetics10.1093/jac/dkg121Pathogenicity
Enzymology23602054Antibacterial serrulatane diterpenes from the Australian native plant Eremophila microtheca.Barnes EC, Kavanagh AM, Ramu S, Blaskovich MA, Cooper MA, Davis RAPhytochemistry2013Anti-Bacterial Agents/chemistry/isolation & purification/*pharmacology, Australia, Diterpenes/chemistry/isolation & purification/*pharmacology, Dose-Response Relationship, Drug, Eremophila Plant/*chemistry, Microbial Sensitivity Tests, Molecular Structure, Staphylococcus/*drug effects, Structure-Activity Relationship10.1016/j.phytochem.2013.02.021Phylogeny
Metabolism30846984Crucial Role of Nucleic Acid Sensing via Endosomal Toll-Like Receptors for the Defense of Streptococcus pyogenes in vitro and in vivo.Hafner A, Kolbe U, Freund I, Castiglia V, Kovarik P, Poth T, Herster F, Weigand MA, Weber ANR, Dalpke AH, Eigenbrod TFront Immunol2019Biomarkers, Cytokines/metabolism, Endosomes/*metabolism, Host-Pathogen Interactions/*immunology, Humans, Immunity, Cellular, Immunity, Innate, Nitric Oxide/metabolism, Nucleic Acids/immunology, RNA, Bacterial/immunology, Reactive Oxygen Species/metabolism, Streptococcal Infections/*immunology/*metabolism/microbiology, Streptococcus pyogenes/*physiology, Toll-Like Receptors/*metabolism10.3389/fimmu.2019.00198Pathogenicity
Genetics32669560Complete genome sequences of Streptococcus pyogenes type strain reveal 100%-match between PacBio-solo and Illumina-Oxford Nanopore hybrid assemblies.Salva-Serra F, Jaen-Luchoro D, Jakobsson HE, Gonzales-Siles L, Karlsson R, Busquets A, Gomila M, Bennasar-Figueras A, Russell JE, Fazal MA, Alexander S, Moore ERBSci Rep2020Bacterial Typing Techniques, Base Sequence, CRISPR-Cas Systems, *Chromosome Mapping, DNA, Bacterial/*genetics/metabolism, Genome, Bacterial, *High-Throughput Nucleotide Sequencing, Nanopores, Prophages/genetics, Sequence Analysis, DNA, Streptococcus pyogenes/classification/*genetics/virology, Virulence Factors/*genetics/metabolism, Whole Genome Sequencing/*methods10.1038/s41598-020-68249-yMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8915Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20565)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20565
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22952P. Lynn Shewmaker, Alvin C. Camus, Tim Bailiff, Arnold G. Steigerwalt, Roger E. Morey, Maria da Glória S. Carvalho10.1099/ijs.0.64810-0Streptococcus ictaluri sp. nov., isolated from Channel Catfish Ictalurus punctatus broodstockIJSEM 57: 1603-1606 200717625202
39803Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/9388
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83875Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13950.1StrainInfo: A central database for resolving microbial strain identifiers
122086Curators of the CIPCollection of Institut Pasteur (CIP 56.41)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2056.41