Strain identifier

BacDive ID: 14734

Type strain: Yes

Species: Streptococcus mitis

Strain Designation: NS 51, SK142

Strain history: <- DSM <- NCTC <- M Kilian, SK142 <- J Carlsson, NS 51

NCBI tax ID(s): 246201 (strain), 28037 (species)

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@ref: 4776

BacDive-ID: 14734

DSM-Number: 12643

keywords: microaerophile, Bacteria, 16S sequence, genome sequence, mesophilic, coccus-shaped

description: Streptococcus mitis NS 51 is a microaerophile, mesophilic, coccus-shaped bacterium that was isolated from oral cavity, human.

NCBI tax id

NCBI tax idMatching level

strain history

doi: 10.13145/bacdive14734.20220920.7

Name and taxonomic classification


  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus mitis
  • full scientific name: Streptococcus mitis Andrewes and Horder 1906 (Approved Lists 1980)

@ref: 4776

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus mitis

full scientific name: Streptococcus mitis Andrewes and Horder 1906 emend. Judicial Commission 1993

strain designation: NS 51, SK142

type strain: yes


cell morphology

@refcell shapegram stain

colony morphology

  • @ref: 4776
  • type of hemolysis: alpha
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

4776COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yes COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
4776TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yes TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
37671MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)

culture temp


Physiology and metabolism

oxygen tolerance

@refoxygen tolerance


  • @ref: 4776
  • murein short key: A11
  • type: A1alpha L-Lys-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837028087glycogen-builds acid from
6837028017starch+builds acid from
6837016634raffinose+builds acid from
6837015443inulin-builds acid from
6837027082trehalose-builds acid from
6837017716lactose+builds acid from
6837017924D-sorbitol-builds acid from
6837016899D-mannitol-builds acid from
6837030849L-arabinose-builds acid from
6837016988D-ribose+builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838116634raffinose+builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
6838128087glycogen-builds acid from
6838127941pullulan+builds acid from
6838117306maltose+builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from

metabolite production


metabolite tests



4776cytochrome-c oxidase-
68381pyrrolidonyl arylamidase-
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381arginine dihydrolase-
68370arginine dihydrolase-
68370leucine arylamidase+
68370alkaline phosphatase-
68370pyrrolidonyl arylamidase-





Isolation, sampling and environmental information


@refsample type
4776oral cavity, human
67770Oral cavity
67771From human oral cavity

isolation source categories

#Host Body-Site#Oral cavity and airways

Safety information

risk assessment

  • @ref: 4776
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptococcus mitis strain ATCC 49456 16S ribosomal RNA gene, partial sequence; and 16S-23S ribosomal RNA intergenic spacer, complete sequenceAY347550373ena28037
20218Streptococcus mitis strain GCSS 1303 16S small subunit ribosomal RNA gene, partial sequenceAY4856011469ena28037
20218Streptococcus mitis 16S rRNA geneD384821403ena28037
20218Streptococcus mitis strain ATCC 49456 16S ribosomal RNA gene, partial sequenceDQ3031881446ena28037
20218Streptococcus mitis strain ATCC 49456 16S ribosomal RNA gene, partial sequenceEU1567601269ena28037
20218Streptococcus mitis strain KCTC 13047 16S ribosomal RNA gene, partial sequenceGU0453871449ena28037
20218Streptococcus mitis strain KCTC 13047 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceHM241934460ena28037
20218Streptococcus mitis strain ATCC 49456 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceJN181387513ena28037
4776Streptococcus mitis 16S ribosomal RNA gene, complete sequenceAF0039291520ena28037
67771Streptococcus mitis JCM 12971 gene for 16S ribosomal RNA, partial sequenceLC3838361486ena28037

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus mitis NCTC 12261 NCTC 12261GCA_000148585completencbi246201
66792Streptococcus mitis NCTC12261GCA_900459425contigncbi28037
66792Streptococcus mitis CCUG 31611GCA_002014755scaffoldncbi28037
66792Streptococcus mitis SK142GCA_019929505contigncbi28037
66792Streptococcus mitis NCTC 12261246201.6wgspatric246201
66792Streptococcus mitis strain CCUG 3161128037.260wgspatric28037
66792Streptococcus mitis strain FDAARGOS 1456 strain Not applicable28037.1427completepatric28037
66792Streptococcus mitis strain NCTC1226128037.464wgspatric28037
66792Streptococcus mitis NCTC 12261 GCA_000148585648276735completeimg246201

GC content


External links

@ref: 4776

culture collection no.: DSM 12643, ATCC 49456, JCM 12971, KCTC 13047, NCTC 12261, CCM 7411, CCUG 31611, CCUG 35790, CIP 103335, KCTC 3556, LMG 14557, NBRC 106071, NCIMB 13770

straininfo link



Enzymology9079939Identification and characterization of IS1381, a new insertion sequence in Streptococcus pneumoniae.Sanchez-Beato AR, Garcia E, Lopez R, Garcia JLJ Bacteriol10.1128/jb.179.7.2459-2463.19971997Amino Acid Sequence, Base Sequence, Blotting, Southern, Cloning, Molecular, *DNA Transposable Elements, DNA, Bacterial/*genetics, Molecular Sequence Data, Open Reading Frames, Repetitive Sequences, Nucleic Acid, Restriction Mapping, Sequence Alignment, Sequence Homology, Amino Acid, Serotyping, Species Specificity, Streptococcus pneumoniae/*geneticsGenetics
Pathogenicity9797205Fluoroquinolone resistance mutations in the parC, parE, and gyrA genes of clinical isolates of viridans group streptococci.Gonzalez I, Georgiou M, Alcaide F, Balas D, Linares J, de la Campa AGAntimicrob Agents Chemother10.1128/AAC.42.11.27921998Amino Acid Sequence, Anti-Infective Agents/*pharmacology, Base Sequence, Ciprofloxacin/pharmacology, DNA Gyrase, DNA Topoisomerase IV, DNA Topoisomerases, Type II/drug effects/*genetics, Drug Resistance, Microbial, *Genes, Bacterial, Humans, Molecular Sequence Data, Mutation, Polymerase Chain Reaction, Streptococcus/*drug effects/genetics, Transformation, GeneticGenetics
Pathogenicity11816366Adhesion in vitro of oral streptococci to porcelain, composite resin cement and human enamel.Takatsuka T, Konishi N, Nakabo S, Hashimoto T, Torii Y, Yoshiyama MDent Mater J10.4012/dmj.19.3632000*Bacterial Adhesion, Dental Enamel/microbiology, Dental Porcelain, Humans, Inlays, Resin Cements, Streptococcus/*physiology, Streptococcus oralis/physiology, Streptococcus sanguis/physiology, Streptococcus sobrinus/physiology
Metabolism17045561Physiological and serological variation in Streptococcus mitis biovar 1 from the human oral cavity during the first year of life.Kirchherr JL, Bowden GH, Cole MF, Kawamura Y, Richmond DA, Sheridan MJ, Wirth KAArch Oral Biol10.1016/j.archoralbio.2006.07.0032006Antigens, Bacterial/immunology, Blotting, Western/methods, Cell Wall/immunology/metabolism, Enzyme-Linked Immunosorbent Assay/methods, Female, Fermentation/physiology, Humans, Immunoglobulin A/immunology, Infant, Infant, Newborn, Male, Mouth/*microbiology, Phenotype, Saliva/enzymology, Serine Endopeptidases/immunology, Streptococcus mitis/enzymology/immunology/*metabolism, Streptococcus oralis/immunology/metabolism, alpha-Amylases/metabolismPathogenicity
Phylogeny21199004Isolation and characterization of Streptococcus mitis from blood of child with osteomyelitis.Nomura R, Nakano K, Makela K, Vaara M, Salo E, Alaluusua S, Ooshima TInt J Paediatr Dent10.1111/j.1365-263X.2010.01110.x20103T3 Cells, Animals, Bacterial Adhesion, Child, Colony Count, Microbial, DNA Fingerprinting, DNA, Bacterial/genetics, Dental Plaque/microbiology, Female, Humans, Mice, Osteoblasts/microbiology, Osteomyelitis/blood/*microbiology, Phagocytosis, Streptococcal Infections/blood/*microbiology, Streptococcus mitis/isolation & purification/*pathogenicityPathogenicity
Phylogeny22357776Streptococcus tigurinus sp. nov., isolated from blood of patients with endocarditis, meningitis and spondylodiscitis.Zbinden A, Mueller NJ, Tarr PE, Sproer C, Keller PM, Bloemberg GVInt J Syst Evol Microbiol10.1099/ijs.0.038299-02012Aged, Bacterial Typing Techniques, Base Composition, Blood/microbiology, DNA, Bacterial/genetics, Discitis/*microbiology, Endocarditis/*microbiology, Humans, Meningitis/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptococcus/*classification/genetics/*isolation & purificationPathogenicity
Pathogenicity23578062Inhibition of attachment of oral bacteria to immortalized human gingival fibroblasts (HGF-1) by tea extracts and tea components.Wang Y, Chung FF, Lee SM, Dykes GABMC Res Notes10.1186/1756-0500-6-1432013Actinomyces/drug effects, Administration, Oral, Bacterial Adhesion/*drug effects, Catechin/analogs & derivatives/pharmacology, Cells, Cultured, Fibroblasts/*microbiology, Flavonoids/chemistry, Gallic Acid/pharmacology, Gingiva/cytology, Humans, Phenol/chemistry, Plant Extracts/pharmacology, Stem Cells, Streptococcus/drug effects, Streptococcus mitis/drug effects, Streptococcus mutans/drug effects, Tannins/chemistry, *Tea
Enzymology27118478Copaifera reticulata oleoresin: Chemical characterization and antibacterial properties against oral pathogens.Bardaji DK, da Silva JJ, Bianchi TC, de Souza Eugenio D, de Oliveira PF, Leandro LF, Rogez HL, Venezianni RC, Ambrosio SR, Tavares DC, Bastos JK, Martins CHAnaerobe10.1016/j.anaerobe.2016.04.0172016Anti-Bacterial Agents/chemistry/isolation & purification/*pharmacology, Bridged Bicyclo Compounds/isolation & purification, Cell Line, Cell Survival/drug effects, Dental Caries/microbiology, Fabaceae/*chemistry, Fibroblasts/cytology/drug effects, Humans, Lactobacillus casei/drug effects/growth & development/isolation & purification, Microbial Sensitivity Tests, Monocyclic Sesquiterpenes, Periodontitis/microbiology, Plant Extracts/chemistry/isolation & purification/*pharmacology, Porphyromonas gingivalis/*drug effects/growth & development/isolation & purification, Prevotella nigrescens/*drug effects/growth & development/isolation & purification, Sesquiterpenes/isolation & purification, Streptococcus mutans/drug effects/growth & development/isolation & purification, Streptococcus salivarius/drug effects/growth & development/isolation & purification, Terpenes/isolation & purificationPhylogeny
Metabolism29669961Aciduricity and acid tolerance mechanisms of Streptococcus anginosus.Sasaki M, Kodama Y, Shimoyama Y, Ishikawa T, Kimura SJ Gen Appl Microbiol10.2323/jgam.2017.11.0052018Acids/*metabolism/pharmacology, Arginine/metabolism, Culture Media, Dicyclohexylcarbodiimide/pharmacology, Gene Deletion, Hydrogen-Ion Concentration, Hydrolases/genetics/*metabolism, Microbial Viability/drug effects, Proton-Translocating ATPases/antagonists & inhibitors/genetics/*metabolism, Streptococcus anginosus/*drug effects/enzymology/genetics/*physiologyCultivation
Phylogeny29898666High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes.Salvadori G, Junges R, Amdal HA, Chen T, Morrison DA, Petersen FCBMC Genomics10.1186/s12864-018-4802-y2018Bacterial Proteins/*physiology, *DNA Transformation Competence, *Gene Expression Regulation, Bacterial, Regulon, Signal Transduction/genetics, Species Specificity, Streptococcus mitis/*genetics/metabolism, Streptococcus pneumoniae/genetics, Up-RegulationMetabolism
Phylogeny31028412Streptococcus gwangjuense sp. nov., Isolated from Human Pericoronitis.Park SN, Lim YK, Shin JH, Chang YH, Shin Y, Paek J, Kim H, Kook JKCurr Microbiol10.1007/s00284-019-01687-82019Base Composition, DNA, Bacterial/genetics, Genome, Bacterial/genetics, Humans, Nucleic Acid Hybridization, Pericoronitis/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Streptococcal Infections/*microbiology, Streptococcus/*classification/cytology/genetics/*physiologyGenetics
Phylogeny31332483Streptococcus chosunense sp. nov., Isolated from Human Postoperative Maxillary Cyst.Lim YK, Park SN, Shin JH, Chang YH, Shin Y, Paek J, Kim H, Kook JKCurr Microbiol10.1007/s00284-019-01746-02019Base Composition, Cysts/*microbiology, DNA, Bacterial/chemistry/genetics, Fatty Acids/chemistry, Genome, Bacterial/genetics, Humans, Male, Maxillary Diseases/*microbiology, Middle Aged, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Species Specificity, Streptococcus/chemistry/*classification/genetics/*physiologyGenetics
Metabolism32825526Cyclic Di-adenosine Monophosphate Regulates Metabolism and Growth in the Oral Commensal Streptococcus mitis.Rorvik GH, Liskiewicz KA, Kryuchkov F, Naemi AO, Aasheim HC, Petersen FC, Kuntziger TM, Simm RMicroorganisms10.3390/microorganisms80912692020
34369916Disinfection of Infected Artificial Dental Periapical Lesions with Diode Laser: An In Vitro Study.Bytyqi A, Aliu X, Barani M, Stubljar D, Jukic T, Starc A, Krasniqi SMed Sci Monit Basic Res10.12659/MSMBR.9324922021Anti-Bacterial Agents, Dental Pulp Cavity, *Disinfection, Enterococcus faecalis, *Lasers, Semiconductor, *Mouth Diseases, Root Canal Therapy
34880844Effects of Expression of Streptococcus pneumoniae PspC on the Ability of Streptococcus mitis to Evade Complement-Mediated Immunity.Marshall H, Jose RJ, Kilian M, Petersen FC, Brown JSFront Microbiol10.3389/fmicb.2021.7738772021
Phylogeny35657449Streptococcus vulneris sp. nov., isolated from wound of patient with diabetic foot ulcer (DFU).Chung JH, Wang LT, Chen YS, Lee YS, Lin ST, Chang YC, Wu HC, Dai YJ, Liao WQ, Kao FC, Li JHArch Microbiol10.1007/s00203-022-02981-z2022Bacterial Typing Techniques, DNA, Bacterial/genetics, *Diabetes Mellitus, *Diabetic Foot, Fatty Acids, Humans, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptococcus/geneticsPathogenicity


4776Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12643)
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37671Curators of the CIP
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCM
67771Curators of the KCTC
68370Automatically annotated from API 20STR
68381Automatically annotated from API rID32STR