Strain identifier

BacDive ID: 14722

Type strain: Yes

Species: Streptococcus constellatus

Strain Designation: 4055

Strain history: CIP <- 1988, NCFB <- 1974, L.V. Holdeman: strain VPI 3810 <- A. Prévot, Inst. Pasteur, Paris, France: strain 4055

NCBI tax ID(s): 76860 (species)

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General

@ref: 8925

BacDive-ID: 14722

DSM-Number: 20575

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, coccus-shaped

description: Streptococcus constellatus 4055 is an anaerobe, mesophilic, coccus-shaped bacterium that was isolated from probably from a case of purulent pleurisy.

NCBI tax id

  • NCBI tax id: 76860
  • Matching level: species

strain history

@refhistory
8925<- ATCC <- L. Holdeman, VPI 3810 <- A. Prévot, 4055
67770CCUG 24889 <-- NCTC 11325 <-- ATCC 27823 <-- L. V. Holdeman VPI 3810 <-- A. Prévot 4055.
67771<- CCUG <- NCTC <- ATCC <- LV Holdeman, VPI 3810 <- A Prevot, 4055
118734CIP <- 1988, NCFB <- 1974, L.V. Holdeman: strain VPI 3810 <- A. Prévot, Inst. Pasteur, Paris, France: strain 4055

doi: 10.13145/bacdive14722.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus constellatus
  • full scientific name: Streptococcus constellatus (Prévot 1924) Holdeman and Moore 1974 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Diplococcus constellatus

@ref: 8925

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus constellatus subsp. constellatus

full scientific name: Streptococcus constellatus subsp. constellatus (Prévot 1924) Whiley et al. 1999

strain designation: 4055

type strain: yes

Morphology

cell morphology

@refcell shapemotilitygram stain
67771coccus-shapedno
67771positive
118734coccus-shapednopositive

colony morphology

  • @ref: 8925
  • type of hemolysis: gamma

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8925COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8925PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
40614MEDIUM 38 - for Streptococcus and Gemella HaemolysansyesDistilled water make up to (1000.000 ml);Horse serum (200.000 ml);Glucose (10.000 g);Brain heart infusion agar (52.000 g)
118734CIP Medium 38yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=38
118734CIP Medium 253yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=253

culture temp

@refgrowthtypetemperaturerange
8925positivegrowth37mesophilic
40614positivegrowth37mesophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8925anaerobe
67771anaerobe
118734obligate aerobe

spore formation

  • @ref: 67771
  • spore formation: no

murein

  • @ref: 8925
  • murein short key: A11.05
  • type: A3alpha L-Lys-L-Ala2

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68370glycogen-builds acid from28087
68370starch-builds acid from28017
68370raffinose-builds acid from16634
68370inulin-builds acid from15443
68370lactose-builds acid from17716
68370D-sorbitol-builds acid from17924
68370D-mannitol-builds acid from16899
68370L-arabinose-builds acid from30849
68370D-ribose-builds acid from16988
68370esculin-hydrolysis4853
68370hippurate-hydrolysis606565
118734nitrate-reduction17632
118734nitrite-reduction16301
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose-builds acid from17716
68381trehalose+builds acid from27082
68381raffinose-builds acid from16634
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

@refChebi-IDmetaboliteproduction
6837015688acetoinyes
11873435581indoleno
6838115688acetoinyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
6837015688acetoin+

enzymes

@refvalueactivityec
8925catalase-1.11.1.6
8925cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase+3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68370leucine arylamidase+3.4.11.1
68370alkaline phosphatase+3.1.3.1
68370beta-galactosidase-3.2.1.23
68370beta-glucuronidase-3.2.1.31
68370alpha-galactosidase-3.2.1.22
68370pyrrolidonyl arylamidase-3.4.19.3
68370beta-glucosidase-3.2.1.21
118734oxidase-
118734catalase-1.11.1.6
118734urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118734-+---+----+----+----

API 20STR

@refVPHIPESCPYRAalpha GALbeta GURbeta GALPALLAPADHRIBARAMANSORLACTREINURAFAMDGLYG
8925+------+++/------+/-----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
118734------------------------------------------------+

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANUREbeta GAL
8925+----+----+-+---++------+--+---
8925-----+----+-+---++------+-------
8925-----++---+-+/-+/---++------+/--------

Isolation, sampling and environmental information

isolation

@refsample type
8925probably from a case of purulent pleurisy
48080Probably from case of purulent pleurisy
67770Probably from a case of purulent pleurisy
67771From probably from a case of purulent pleurisy
118734Human, Purulent pleurisy

isolation source categories

Cat1Cat2
#Infection#Inflammation
#Host Body-Site#Oral cavity and airways

taxonmaps

  • @ref: 69479
  • File name: preview.99_1281.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_456;97_522;98_1012;99_1281&stattab=map
  • Last taxonomy: Streptococcus
  • 16S sequence: LC383837
  • Sequence Identity:
  • Total samples: 35400
  • soil counts: 275
  • aquatic counts: 764
  • animal counts: 34119
  • plant counts: 242

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
89252Risk group (German classification)
1187342Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptococcus constellatus gene for 16S ribosomal RNA, partial sequence, strain: ATCC 27823AB3556051399ena76860
20218Streptococcus constellatus strain ATCC27823 16S ribosomal RNA gene, partial sequenceAF1046761558ena76860
20218Streptococcus constellatus subsp. constellatus strain ATCC 27823 16S-23S ribosomal RNA intergenic spacer, complete sequenceAY347546316ena184246
20218S.constellatus 16S ribosomal RNAZ69041517ena76860
20218Streptococcus constellatus 16S-23S ribosomal RNA intergenic spacer and transfer RNA-AlaL36933319ena76860
20218Streptococcus constellatus subsp. constellatus strain NCTC 11325 16S-23S ribosomal RNA intergenic spacer, partial sequenceEU860343396ena184246
20218Streptococcus constellatus subsp. constellatus strain SK53 16S ribosomal RNA gene, partial sequenceJN7871361404ena184246
67771Streptococcus constellatus subsp. constellatus JCM 12994 gene for 16S ribosomal RNA, partial sequenceLC3838371494ena184246

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus constellatus NCTC11325GCA_900459125contigncbi76860
66792Streptococcus constellatus subsp. constellatus ATCC 27823GCA_008633005scaffoldncbi184246
66792Streptococcus constellatus strain FDAARGOS_115676860.28completepatric76860
66792Streptococcus constellatus strain NCTC1132576860.11wgspatric76860
66792Streptococcus constellatus subsp. constellatus strain ATCC 27823184246.4wgspatric184246
66792Streptococcus constellatus subsp. constellatus CCUG 24889GCA_023167545completencbi184246

GC content

@refGC-content
892536.8
6777037-38

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno97.147yes
flagellatedno97.539yes
gram-positiveyes94.68yes
anaerobicno94.049no
halophileyes78.061no
spore-formingno95.134yes
glucose-utilyes89.62no
aerobicno97.483yes
thermophileno99.383yes
glucose-fermentyes86.093no

External links

@ref: 8925

culture collection no.: DSM 20575, ATCC 27823, CIP 103247, CCUG 24889, NCTC 11325, LMG 14507, JCM 12994, KCTC 15405, VPI 3810, NCDO 2226, NCIMB 702226

straininfo link

  • @ref: 83840
  • straininfo: 92729

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity1995029Emended descriptions and recognition of Streptococcus constellatus, Streptococcus intermedius, and Streptococcus anginosus as distinct species.Whiley RA, Beighton DInt J Syst Bacteriol10.1099/00207713-41-1-11991Base Composition, DNA, Bacterial/analysis, Humans, Serotyping, Streptococcus/*classification/cytology/genetics/physiologyPhylogeny
Pathogenicity2111871Serogrouping of oral Streptococcus intermedius.Osano E, Tanaka T, Ozeki M, Makita I, Moriyama TMicrobiol Immunol1990Animals, Antigens, Bacterial/*analysis, Bacterial Typing Techniques, Humans, Immunodiffusion, Male, Mouth Diseases/complications/*microbiology, Rabbits, Streptococcal Infections/complications/*immunology, Streptococcus/*classification/immunology/isolation & purificationPhylogeny
Pathogenicity2610299[Taxonomic studies of the Streptococcus intermedius strains isolated from human oral cavities].Fujii YAichi Gakuin Daigaku Shigakkai Shi1989Humans, Mouth/*microbiology, Streptococcus/*classificationPhylogeny
Phylogeny2632666DNA-DNA hybridization studies and phenotypic characteristics of strains within the 'Streptococcus milleri group'.Whiley RA, Hardie JMJ Gen Microbiol10.1099/00221287-135-10-26231989Bacterial Proteins/isolation & purification, DNA, Bacterial/*genetics, Electrophoresis, Polyacrylamide Gel, Nucleic Acid Hybridization, Phenotype, Sequence Homology, Nucleic Acid, Sodium Dodecyl Sulfate, Species Specificity, Streptococcus/analysis/classification/*geneticsEnzymology
Phylogeny8784576Rapid species identification of "Streptococcus milleri" strains by line blot hybridization: identification of a distinct 16S rRNA population closely related to Streptococcus constellatus.Jacobs JA, Schot CS, Bunschoten AE, Schouls LMJ Clin Microbiol10.1128/JCM.34.7.1717-1721.19961996Bacterial Typing Techniques, Base Sequence, DNA Probes/genetics, Genes, Bacterial, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Polymerase Chain Reaction, RNA, Bacterial/*genetics, RNA, Ribosomal, 16S/*genetics, Species Specificity, Streptococcus/*classification/*genetics/isolation & purificationPathogenicity
Phylogeny10826810DNA-DNA reassociation studies of Streptococcus constellatus with unusual 16S rRNA sequences.Jacobs JA, Schouls LM, Whiley RAInt J Syst Evol Microbiol10.1099/00207713-50-1-2472000DNA, Bacterial/*genetics, Genes, rRNA, *Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/*genetics, Streptococcus/*classification/genetics
Phylogeny31053905Streptococcus periodonticum sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion.Lim YK, Park SN, Shin JH, Chang YH, Shin Y, Paek J, Kim H, Kook JKCurr Microbiol10.1007/s00284-019-01695-82019Bacteria, Anaerobic, Bacterial Proteins/genetics, Base Composition, DNA, Bacterial/genetics, DNA-Directed RNA Polymerases/genetics, Dental Plaque/*microbiology, Fatty Acids/analysis, Genome, Bacterial/genetics, Humans, Periodontitis/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Streptococcus/*classification/genetics/*physiology, Superoxide Dismutase/geneticsGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8925Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20575)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20575
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40614Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15024
48080Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 24889)https://www.ccug.se/strain?id=24889
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68370Automatically annotated from API 20STR
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83840Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92729.1StrainInfo: A central database for resolving microbial strain identifiers
118734Curators of the CIPCollection of Institut Pasteur (CIP 103247)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103247