Strain identifier

BacDive ID: 14516

Type strain: Yes

Species: Staphylococcus carnosus

Strain Designation: 361

Strain history: CIP <- 1989, DSM, Staphylococcus carnosus <- K.H. Schleifer, Germany

NCBI tax ID(s): 1281 (species)

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General

@ref: 8743

BacDive-ID: 14516

DSM-Number: 20501

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive

description: Staphylococcus carnosus 361 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from dry sausage.

NCBI tax id

  • NCBI tax id: 1281
  • Matching level: species

strain history

@refhistory
8743<- K.H. Schleifer, 361
117190CIP <- 1989, DSM, Staphylococcus carnosus <- K.H. Schleifer, Germany

doi: 10.13145/bacdive14516.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus carnosus
  • full scientific name: Staphylococcus carnosus Schleifer and Fischer 1982

  • synonyms

    • @ref: 20215
    • synonym: Staphylococcus carnosus subsp. utilis

@ref: 8743

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus carnosus

full scientific name: Staphylococcus carnosus Schleifer and Fischer 1982

strain designation: 361

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
117190positivecoccus-shapedno

colony morphology

@reftype of hemolysisincubation period
8743gamma1-2 days
117190

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8743COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8743TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34236MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
117190CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
8743positivegrowth37mesophilic
34236positivegrowth37mesophilic
117190positivegrowth22-45

Physiology and metabolism

oxygen tolerance

  • @ref: 117190
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
69481no93
69480no99.463

murein

  • @ref: 8743
  • murein short key: A11.02
  • type: A3alpha L-Lys-Gly5-6

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
117190hippurate-hydrolysis606565
117190nitrate+reduction17632
117190nitrite-reduction16301
68375urea-hydrolysis16199
68375ornithine-degradation18257
68375D-glucose+fermentation17634
68375D-fructose+fermentation15824
68375D-mannose+fermentation16024
68375maltose-fermentation17306
68375lactose+fermentation17716
68375D-mannitol+fermentation16899
68375raffinose-fermentation16634
68375D-ribose-fermentation16988
68375cellobiose-fermentation17057
68375nitrate+reduction17632
68375sucrose-fermentation17992
68375turanose-fermentation32528
68375arabinose-fermentation22599

metabolite production

  • @ref: 117190
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 117190
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
8743catalase+1.11.1.6
8743cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase-3.2.1.31
68375L-arginine arylamidase-
68375beta-galactosidase+3.2.1.23
68375ornithine decarboxylase-4.1.1.17
68375urease-3.5.1.5
117190oxidase-
117190beta-galactosidase+3.2.1.23
117190alcohol dehydrogenase+1.1.1.1
117190gelatinase-
117190amylase-
117190DNase-
117190caseinase-3.4.21.50
117190catalase+1.11.1.6
117190coagulase-
117190tween esterase-
117190gamma-glutamyltransferase-2.3.2.2
117190lecithinase-
117190lipase-
117190lysine decarboxylase-4.1.1.18
117190ornithine decarboxylase-4.1.1.17
117190tryptophan deaminase-
117190urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117190-+++------++-+-+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117190+/----+/-----+/-+++----++--+/-------+/---+-----------------

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8743-+-++++-+++---+++-+++-+---
8743-+--+++-+++---+-+-+++-+/----
8743-+--+++-+++---+-+-+++-----
8743-+--+++-+++---+++-+++-+---
8743----+++-+++---+-+-+/--+-+---
8743-+--+++-++/-+---+-+-+++/------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117190++-++------+-------+-----------+-+--+---+------------------++--------------+----------+---+++++----

Isolation, sampling and environmental information

isolation

@refsample typesampling date
8743dry sausage
46290Dry sausage1983-10-07
117190Food, Dry sausage

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Meat

taxonmaps

  • @ref: 69479
  • File name: preview.99_1206.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3;97_3;98_955;99_1206&stattab=map
  • Last taxonomy: Staphylococcus
  • 16S sequence: AB009934
  • Sequence Identity:
  • Total samples: 22504
  • soil counts: 1443
  • aquatic counts: 1299
  • animal counts: 19130
  • plant counts: 632

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
87431Risk group (German classification)
1171901Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus carnosus 16S ribosomal RNA gene, partial sequenceAF041356913ena1281
20218Staphylococcus carnosus subsp. carnosus strain DSM 20501 16S ribosomal RNA gene, partial sequenceAY688041464ena147448
20218S.carnosus gene for 16S ribosomal RNAZ26891771ena1281
8743Staphylococcus carnosus rrn gene for 16S ribosomal RNAAB0099341493ena1281

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus carnosus strain FDAARGOS_8831281.24completepatric1281
66792Staphylococcus carnosus subsp. carnosus strain DSM 20501147448.20wgspatric1281

GC content

  • @ref: 8743
  • GC-content: 36.0

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno93no
motileno87.908no
gram-positiveyes89.975no
anaerobicno99.123no
aerobicyes90.438no
halophileyes93.404no
spore-formingno92.354no
thermophileno99.83yes
glucose-utilyes89.21no
flagellatedno89.259no
glucose-fermentyes80.898no

External links

@ref: 8743

culture collection no.: DSM 20501, ATCC 51365, CCUG 15605, CIP 103274, GIFU 10766

straininfo link

  • @ref: 83647
  • straininfo: 46150

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10425749Complex genomic and phenotypic characterization of the related species Staphylococcus carnosus and Staphylococcus piscifermentans.Pantucek R, Sedlacek I, Doskar J, Rosypal SInt J Syst Bacteriol10.1099/00207713-49-3-9411999*Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics/isolation & purification, DNA, Ribosomal/genetics, Deoxyribonuclease EcoRI/metabolism, Electrophoresis, Gel, Pulsed-Field, Genotype, Phenotype, Polymerase Chain Reaction/methods, RNA, Ribosomal/genetics, Restriction Mapping, Species Specificity, Staphylococcus/*classification/*geneticsEnzymology
Pathogenicity29446685Inactivation of Salmonella and Surrogate Bacteria on Cashews and Macadamia Nuts Exposed to Commercial Propylene Oxide Processing Conditions.Saunders T, Wu J, Williams RC, Huang H, Ponder MAJ Food Prot10.4315/0362-028X.JFP-17-2522018Anacardium/*microbiology, Epoxy Compounds/*pharmacology, Macadamia/*microbiology, Nuts/*microbiology, Salmonella/*drug effects

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8743Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20501)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20501
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34236Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15053
46290Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 15605)https://www.ccug.se/strain?id=15605
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83647Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46150.1StrainInfo: A central database for resolving microbial strain identifiers
117190Curators of the CIPCollection of Institut Pasteur (CIP 103274)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103274