Strain identifier

BacDive ID: 14444

Type strain: Yes

Species: Staphylococcus aureus

Strain history: <- K.H. Schleifer <- R. De la Fuente, MVF 7

NCBI tax ID(s): 1280 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 9024

BacDive-ID: 14444

DSM-Number: 20714

keywords: human pathogen, mesophilic, microaerophile, Bacteria, 16S sequence, genome sequence

description: Staphylococcus aureus DSM 20714 is a microaerophile, mesophilic human pathogen that was isolated from abscess of young sheep.

NCBI tax id

  • NCBI tax id: 1280
  • Matching level: species

strain history: <- K.H. Schleifer <- R. De la Fuente, MVF 7

doi: 10.13145/bacdive14444.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus aureus
  • full scientific name: Staphylococcus aureus Rosenbach 1884 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Staphylococcus aureus subsp. anaerobius

@ref: 9024

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus aureus subsp. anaerobius

full scientific name: Staphylococcus aureus subsp. anaerobius De La Fuente et al. 1985

type strain: yes

Morphology

colony morphology

  • @ref: 9024
  • type of hemolysis: beta
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9024COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://bacmedia.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
9024TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://bacmedia.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
38147MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)

culture temp

@refgrowthtypetemperaturerange
9024positivegrowth37mesophilic
38147positivegrowth37mesophilic
53551positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
9024microaerophile
53551aerobe
53551microaerophile

murein

  • @ref: 9024
  • murein short key: A11.02
  • type: A3alpha L-Lys-Gly5-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837522599arabinose-fermentation
6837532528turanose-fermentation
6837517992sucrose+fermentation
6837517057cellobiose-fermentation
6837516988D-ribose-fermentation
6837516634raffinose-fermentation
6837516899D-mannitol-fermentation
6837527082trehalose-fermentation
6837517716lactose-fermentation
6837517306maltose-fermentation
6837517634D-glucose+fermentation
683754853esculin-hydrolysis
6837518257ornithine-degradation
6837529016arginine+hydrolysis
6837516199urea-hydrolysis

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is resistant: yes
  • resistance conc.: 1.8 µg

metabolite production

  • @ref: 68375
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

  • @ref: 68375
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
9024catalase+1.11.1.6
9024cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375alkaline phosphatase+3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase+3.5.3.6
68375urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    53551C14:00.514
    53551C16:03.316
    53551C18:07.318
    53551C19:00.619
    53551C20:04.920
    53551C13:0 iso0.712.612
    53551C14:0 ISO1.113.618
    53551C15:0 ANTEISO52.314.711
    53551C15:0 ISO814.621
    53551C16:0 iso0.715.626
    53551C17:0 anteiso6.716.722
    53551C17:0 iso2.616.629
    53551C18:1 ω9c1.117.769
    53551C18:2 ω6,9c/C18:0 ANTE4.617.724
    53551C19:0 ANTEISO1.818.729
    53551C19:0 ISO118.633
    53551C20:2 ω6,9c2.219.735
    53551Unidentified0.317.943
    53551Unidentified0.319.947
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
9024-+--++/---------+---+-++----
9024-+--++------------+-++----
9024-+--+++-----------+-+++/----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
9024abscess of young sheep
53551Sheep abscess,young animalMadridSpainESPEurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Caprinae (Sheep/Goat)
#Host Body-Site#Other#Abscess

Safety information

risk assessment

  • @ref: 9024
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus aureus subsp. anaerobius strain DSM 20714 16S ribosomal RNA gene, partial sequenceAY688031464ena72759
9024Staphylococcus aureus subsp. anaerobius gene for 16S rRNA, partial sequence, strain: ATCC 35844D833551476ena72759

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus aureus DSM 20714GCA_002902425scaffoldncbi1280
66792Staphylococcus aureus subsp. anaerobius strain DSM 2071472759.14wgspatric1280
66792Staphylococcus aureus anaerobius DSM 207142917488298draftimg1280

GC content

  • @ref: 9024
  • GC-content: 31.7

External links

@ref: 9024

culture collection no.: DSM 20714, ATCC 35844, CIP 103780, CCUG 37246, CCM 3823, MVF 7

straininfo link

@refpassport
20218http://www.straininfo.net/strains/155219
20218http://www.straininfo.net/strains/111382
20218http://www.straininfo.net/strains/155217

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics20526831The catalase gene differentiates between some strains of Staphylococcus aureus ssp. anaerobius.Musa NO, Eltom K, Gessler F, Bohnel H, Babiker A, El Sanousi SMFolia Microbiol (Praha)10.1007/s12223-010-0030-92010Abscess/epidemiology/microbiology/veterinary, Amino Acid Substitution/genetics, Animals, Bacterial Proteins/*genetics, Catalase/*genetics, Codon, Nonsense, DNA, Bacterial/chemistry/genetics, Disease Outbreaks, Molecular Sequence Data, *Polymorphism, Genetic, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sheep, Sheep Diseases/epidemiology/microbiology, Staphylococcal Infections/epidemiology/microbiology/veterinary, Staphylococcus aureus/*classification/*enzymology/isolation & purificationTranscriptome
Genetics21462863Phenotypic and genotypic properties of Staphylococcus aureus subsp. anaerobius isolated from lymph node abscesses of goats.Szalus-Jordanow O, Kanbar T, Soedarmanto I, Eissa N, Alber J, Lammler C, Zschock M, Weiss R, Kaba J, Frymus TBerl Munch Tierarztl Wochenschr2011Abscess/*microbiology/veterinary, Animals, Base Sequence, Genes, Bacterial/genetics, Genotype, Goat Diseases/*microbiology, Goats, Lymph Nodes/*microbiology, Molecular Sequence Data, *Phenotype, Poland, Sequence Alignment, Staphylococcal Infections/microbiology/*veterinary, Staphylococcus aureus/*genetics/isolation & purification/*metabolismPhylogeny
Phylogeny22976748PFGE and AFLP genotyping of Staphylococcus aureus subsp. anaerobius isolated from goats with Morel's disease.Szalus-Jordanow O, Chrobak D, Pyrgiel M, Lutynska A, Kaba J, Czopowicz M, Witkowski L, Kizerwetter-Swida M, Binek M, Frymus TArch Microbiol10.1007/s00203-012-0844-82012Abscess/microbiology/*veterinary, *Amplified Fragment Length Polymorphism Analysis, Animals, *Electrophoresis, Gel, Pulsed-Field, Genotype, Goat Diseases/*microbiology, Goats, Phylogeny, Poland, Staphylococcal Infections/microbiology/*veterinary, Staphylococcus aureus/*classification/*genetics/isolation & purificationEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9024Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20714)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20714
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38147Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15615
53551Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37246)https://www.ccug.se/strain?id=37246
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68375Automatically annotated from API ID32STA