Strain identifier

BacDive ID: 14444

Type strain: Yes

Species: Staphylococcus aureus

Strain Designation: MVF-7

Strain history: CIP <- 2008, DSMZ <- K.H. Schleifer, Inst. Botanik und Microbiol. Tech. Univ., Munich, Germany <- 1993, G.S. Fernandez, Ciudad Univ., Madrid, Spain <- R. de La Fuente Lopez, Madrid Univ., Spain: strain MVF-7

NCBI tax ID(s): 1280 (species)

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General

@ref: 9024

BacDive-ID: 14444

DSM-Number: 20714

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, human pathogen

description: Staphylococcus aureus MVF-7 is a microaerophile, mesophilic, Gram-positive human pathogen that was isolated from abscess of young sheep.

NCBI tax id

  • NCBI tax id: 1280
  • Matching level: species

strain history

@refhistory
9024<- K.H. Schleifer <- R. De la Fuente, MVF 7
116575CIP <- 2008, DSMZ <- K.H. Schleifer, Inst. Botanik und Microbiol. Tech. Univ., Munich, Germany <- 1993, G.S. Fernandez, Ciudad Univ., Madrid, Spain <- R. de La Fuente Lopez, Madrid Univ., Spain: strain MVF-7

doi: 10.13145/bacdive14444.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus aureus
  • full scientific name: Staphylococcus aureus Rosenbach 1884 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Staphylococcus aureus subsp. anaerobius

@ref: 9024

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus aureus subsp. anaerobius

full scientific name: Staphylococcus aureus subsp. anaerobius De La Fuente et al. 1985

strain designation: MVF-7

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
116575positivecoccus-shapedno

colony morphology

@reftype of hemolysishemolysis abilityincubation period
9024beta11-2 days
116575

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9024COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
9024TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
38147MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116575CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
116575CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
9024positivegrowth37mesophilic
38147positivegrowth37mesophilic
53551positivegrowth37mesophilic
116575positivegrowth37mesophilic
116575nogrowth15psychrophilic
116575nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
9024microaerophile
53551aerobe
53551microaerophile
116575facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.695

murein

  • @ref: 9024
  • murein short key: A11.02
  • type: A3alpha L-Lys-Gly5-6

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
116575nitrate-reduction17632
116575nitrite-reduction16301
68375urea-hydrolysis16199
68375arginine+hydrolysis29016
68375ornithine-degradation18257
68375esculin-hydrolysis4853
68375D-glucose+fermentation17634
68375maltose-fermentation17306
68375lactose-fermentation17716
68375trehalose-fermentation27082
68375D-mannitol-fermentation16899
68375raffinose-fermentation16634
68375D-ribose-fermentation16988
68375cellobiose-fermentation17057
68375sucrose+fermentation17992
68375turanose-fermentation32528
68375arabinose-fermentation22599

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is resistant: yes
  • resistance conc.: 1.8 µg

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinno
11657535581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6837515688acetoin-
11657515688acetoin-

enzymes

@refvalueactivityec
9024catalase+1.11.1.6
9024cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375alkaline phosphatase+3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase+3.5.3.6
68375urease-3.5.1.5
116575oxidase-
116575beta-galactosidase+3.2.1.23
116575alcohol dehydrogenase+1.1.1.1
116575gelatinase+/-
116575DNase+
116575catalase-1.11.1.6
116575coagulase+
116575lecithinase+
116575lysine decarboxylase-4.1.1.18
116575ornithine decarboxylase-4.1.1.17
116575urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    53551C14:00.514
    53551C16:03.316
    53551C18:07.318
    53551C19:00.619
    53551C20:04.920
    53551C13:0 iso0.712.612
    53551C14:0 ISO1.113.618
    53551C15:0 ANTEISO52.314.711
    53551C15:0 ISO814.621
    53551C16:0 iso0.715.626
    53551C17:0 anteiso6.716.722
    53551C17:0 iso2.616.629
    53551C18:1 ω9c1.117.769
    53551C18:2 ω6,9c/C18:0 ANTE4.617.724
    53551C19:0 ANTEISO1.818.729
    53551C19:0 ISO118.633
    53551C20:2 ω6,9c2.219.735
    53551Unidentified0.317.943
    53551Unidentified0.319.947
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116575-+++-+---+++++--+---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116575----+----++++--------+++++++++++--+---+----------

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
9024-+--++/---------+---+-++----
9024-+--++------------+-++----
9024-+--+++-----------+-+++/----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
9024abscess of young sheep
53551Sheep abscess,young animalMadridSpainESPEurope
116575Animal, Young sheep, abscessMadridSpainESPEurope1984

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Caprinae (Sheep/Goat)
#Host Body-Site#Other#Abscess

taxonmaps

  • @ref: 69479
  • File name: preview.99_3.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3;97_3;98_3;99_3&stattab=map
  • Last taxonomy: Staphylococcus
  • 16S sequence: D83355
  • Sequence Identity:
  • Total samples: 23199
  • soil counts: 353
  • aquatic counts: 991
  • animal counts: 21663
  • plant counts: 192

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
9024yesyes2Risk group (German classification)
1165752Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus aureus subsp. anaerobius strain DSM 20714 16S ribosomal RNA gene, partial sequenceAY688031464ena72759
9024Staphylococcus aureus subsp. anaerobius gene for 16S rRNA, partial sequence, strain: ATCC 35844D833551476ena72759

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus aureus DSM 20714GCA_002902425scaffoldncbi1280
66792Staphylococcus aureus subsp. anaerobius strain DSM 2071472759.14wgspatric1280
66792Staphylococcus aureus anaerobius DSM 207142917488298draftimg1280

GC content

  • @ref: 9024
  • GC-content: 31.7

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno91.301no
gram-positiveyes89.346no
anaerobicno99.425no
aerobicyes88.145no
halophileyes93.859no
spore-formingno93.35no
glucose-utilyes88.473no
thermophileno99.66yes
motileno89.349no
glucose-fermentyes84.981no

External links

@ref: 9024

culture collection no.: DSM 20714, ATCC 35844, CIP 103780, CCUG 37246, CCM 3823, MVF 7, GIFU 10707

straininfo link

  • @ref: 83587
  • straininfo: 41328

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics20526831The catalase gene differentiates between some strains of Staphylococcus aureus ssp. anaerobius.Musa NO, Eltom K, Gessler F, Bohnel H, Babiker A, El Sanousi SMFolia Microbiol (Praha)10.1007/s12223-010-0030-92010Abscess/epidemiology/microbiology/veterinary, Amino Acid Substitution/genetics, Animals, Bacterial Proteins/*genetics, Catalase/*genetics, Codon, Nonsense, DNA, Bacterial/chemistry/genetics, Disease Outbreaks, Molecular Sequence Data, *Polymorphism, Genetic, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sheep, Sheep Diseases/epidemiology/microbiology, Staphylococcal Infections/epidemiology/microbiology/veterinary, Staphylococcus aureus/*classification/*enzymology/isolation & purificationTranscriptome
Genetics21462863Phenotypic and genotypic properties of Staphylococcus aureus subsp. anaerobius isolated from lymph node abscesses of goats.Szalus-Jordanow O, Kanbar T, Soedarmanto I, Eissa N, Alber J, Lammler C, Zschock M, Weiss R, Kaba J, Frymus TBerl Munch Tierarztl Wochenschr2011Abscess/*microbiology/veterinary, Animals, Base Sequence, Genes, Bacterial/genetics, Genotype, Goat Diseases/*microbiology, Goats, Lymph Nodes/*microbiology, Molecular Sequence Data, *Phenotype, Poland, Sequence Alignment, Staphylococcal Infections/microbiology/*veterinary, Staphylococcus aureus/*genetics/isolation & purification/*metabolismPhylogeny
Phylogeny22976748PFGE and AFLP genotyping of Staphylococcus aureus subsp. anaerobius isolated from goats with Morel's disease.Szalus-Jordanow O, Chrobak D, Pyrgiel M, Lutynska A, Kaba J, Czopowicz M, Witkowski L, Kizerwetter-Swida M, Binek M, Frymus TArch Microbiol10.1007/s00203-012-0844-82012Abscess/microbiology/*veterinary, *Amplified Fragment Length Polymorphism Analysis, Animals, *Electrophoresis, Gel, Pulsed-Field, Genotype, Goat Diseases/*microbiology, Goats, Phylogeny, Poland, Staphylococcal Infections/microbiology/*veterinary, Staphylococcus aureus/*classification/*genetics/isolation & purificationEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9024Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20714)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20714
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38147Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15615
53551Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37246)https://www.ccug.se/strain?id=37246
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83587Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID41328.1StrainInfo: A central database for resolving microbial strain identifiers
116575Curators of the CIPCollection of Institut Pasteur (CIP 103780)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103780