Strain identifier

BacDive ID: 14403

Type strain: Yes

Species: Sporolactobacillus kofuensis

Strain Designation: M-19

Strain history: CIP <- 1997, JCM <- F. Yanagida, Yamanashi Univ., Japan <- O. Nakayama: strain M-19

NCBI tax ID(s): 269672 (species)

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General

@ref: 7088

BacDive-ID: 14403

DSM-Number: 17615

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, motile, rod-shaped

description: Sporolactobacillus kofuensis M-19 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 269672
  • Matching level: species

strain history

@refhistory
7088<- RIKEN BRC-JCM, Japan; JCM 3419 <- F. Yanagida; <- O. Nakayama; M-19
120458CIP <- 1997, JCM <- F. Yanagida, Yamanashi Univ., Japan <- O. Nakayama: strain M-19

doi: 10.13145/bacdive14403.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Sporolactobacillaceae
  • genus: Sporolactobacillus
  • species: Sporolactobacillus kofuensis
  • full scientific name: Sporolactobacillus kofuensis Yanagida et al. 1997

@ref: 7088

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Sporolactobacillaceae

genus: Sporolactobacillus

species: Sporolactobacillus kofuensis

full scientific name: Sporolactobacillus kofuensis Yanagida et al. 1997

strain designation: M-19

type strain: yes

Morphology

cell morphology

  • @ref: 120458
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7088GYP GLUCOSE-YEAST-PEPTONE MEDIUM (DSMZ Medium 852)yeshttps://mediadive.dsmz.de/medium/852Name: GYP GLUCOSE-YEAST-PEPTONE MEDIUM (DSMZ Medium 852) Composition: Glucose 20.0 g/l Yeast extract 10.0 g/l Peptone 10.0 g/l Na-acetate 10.0 g/l Agar 10.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x 4 H2O 0.01 g/l FeSO4 x 7 H2O 0.01 g/l NaCl 0.01 g/l Distilled water
39264MEDIUM 217 - for SporolactobacillusyesDistilled water make up to (1000.000 ml);Sodium chloride (0.100 g);Manganese II sulphate monohydrate (0.160 g);Magnesium sulphate heptahydrate (2.000 g);Glucose (20.000 g);Yeast extract (10.000 g);Peptone (10.000 g);Sodium acetate (10.000 g);Iron (III) su
7088R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
120458CIP Medium 260yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=260
120458CIP Medium 217yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=217

culture temp

@refgrowthtypetemperaturerange
7088positivegrowth30mesophilic
39264positivegrowth30mesophilic
120458positivegrowth25-30mesophilic
120458nogrowth15psychrophilic
120458nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
7088microaerophile
120458facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
120458NaClpositivegrowth0 %
120458NaClnogrowth2 %
120458NaClnogrowth4 %
120458NaClnogrowth6 %
120458NaClnogrowth8 %
120458NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin+builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120458esculin-hydrolysis4853
120458nitrate-reduction17632
120458nitrite-reduction16301
120458nitrate+respiration17632

antibiotic resistance

  • @ref: 120458
  • ChEBI: 6909
  • metabolite: metronidazole
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

enzymes

@refvalueactivityec
120458oxidase-
120458beta-galactosidase-3.2.1.23
120458gelatinase-
120458amylase-
120458DNase-
120458caseinase-3.4.21.50
120458catalase-1.11.1.6
120458tween esterase-
120458lecithinase-
120458lipase-
120458urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120458--++-+----++---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120458----------+++----++-+/-+-----+--+++-+++--+-+------+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7088soilKofu, YamanashiJapanJPNAsia
120458Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_34211.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_414;96_3495;97_4284;98_5485;99_34211&stattab=map
  • Last taxonomy: Sporolactobacillus
  • 16S sequence: AB374517
  • Sequence Identity:
  • Total samples: 108
  • soil counts: 31
  • aquatic counts: 8
  • animal counts: 56
  • plant counts: 13

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
70881Risk group (German classification)
1204581Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Sporolactobacillus kofuensis genes for 16S rRNA, ITS1, 23S rRNA, partial and complete sequence, strain: JCM 3419AB231793459ena269672
20218Sporolactobacillus kofuensis gene for 16S ribosomal RNA, partial sequenceAB3745171503ena269672
20218Sporolactobacillus kofuensis gene for 16S rRNA, partial sequenceAB3716351476ena269672

GC content

  • @ref: 7088
  • GC-content: 43

External links

@ref: 7088

culture collection no.: DSM 17615, JCM 3419, CIP 105320

straininfo link

  • @ref: 83547
  • straininfo: 12435

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7088Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17615)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17615
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39264Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17327
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83547Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12435.1StrainInfo: A central database for resolving microbial strain identifiers
120458Curators of the CIPCollection of Institut Pasteur (CIP 105320)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105320