Strain identifier
BacDive ID: 14378
Type strain: 
Species: Spiroplasma platyhelix
Strain Designation: PALS-1
Strain history: <- J. K. Davis, Dept. Agronomy, Purdue Univ., West Lafayette, USA <- J. G. Tully <- K. J. Hackett, USDA
NCBI tax ID(s): 1276218 (strain), 301585 (species)
version 8.1 (current version)
General
@ref: 16413
BacDive-ID: 14378
DSM-Number: 22601
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic
description: Spiroplasma platyhelix PALS-1 is a microaerophile, mesophilic bacterium that was isolated from gut of the dragonfly Pachydiplax longipennis.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 1276218 | strain |
| 301585 | species |
strain history
- @ref: 16413
- history: <- J. K. Davis, Dept. Agronomy, Purdue Univ., West Lafayette, USA <- J. G. Tully <- K. J. Hackett, USDA
doi: 10.13145/bacdive14378.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/mycoplasmatota
- domain: Bacteria
- phylum: Mycoplasmatota
- class: Mollicutes
- order: Mycoplasmatales
- family: Spiroplasmataceae
- genus: Spiroplasma
- species: Spiroplasma platyhelix
- full scientific name: Spiroplasma platyhelix Williamson et al. 1997
@ref: 16413
domain: Bacteria
phylum: Tenericutes
class: Mollicutes
order: Entomoplasmatales
family: Spiroplasmataceae
genus: Spiroplasma
species: Spiroplasma platyhelix
full scientific name: Spiroplasma platyhelix Williamson et al. 1997
strain designation: PALS-1
type strain: yes
Morphology
cell morphology
- @ref: 69480
- motility: no
- confidence: 93.62
Culture and growth conditions
culture medium
- @ref: 16413
- name: SP4-Z MEDIUM (DSMZ Medium 1076b)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1076b
- composition: Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water
culture temp
- @ref: 16413
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 16413
- oxygen tolerance: microaerophile
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.999 |
Isolation, sampling and environmental information
isolation
- @ref: 16413
- sample type: gut of the dragonfly Pachydiplax longipennis
- host species: Pachydiplax longipennis
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Arthropoda | #Insecta |
| #Host Body-Site | #Gastrointestinal tract | #Stomach |
taxonmaps
- @ref: 69479
- File name: preview.99_64138.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4828;96_27909;97_35140;98_45363;99_64138&stattab=map
- Last taxonomy: Spiroplasma platyhelix subclade
- 16S sequence: GU993266
- Sequence Identity:
- Total samples: 290
- soil counts: 60
- aquatic counts: 23
- animal counts: 202
- plant counts: 5
Safety information
risk assessment
- @ref: 16413
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Spiroplasma platyhelix strain ATCC 51748 16S ribosomal RNA gene, partial sequence | GU993266 | 1477 | ena | 1276218 |
| 16413 | Spiroplasma platyhelix strain PALS-1 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | DQ860101 | 2216 | ena | 1276218 |
| 16413 | Spiroplasma platyhelix strain PALS-1 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | AY800347 | 877 | ena | 1276218 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Spiroplasma platyhelix PALS-1 | GCA_012163225 | contig | ncbi | 1276218 |
| 66792 | Spiroplasma platyhelix PALS-1 | 1276218.3 | wgs | patric | 1276218 |
| 66792 | Spiroplasma platyhelix PALS-1 | GCA_021496725 | complete | ncbi | 1276218 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| flagellated | no | 97.76 | no |
| gram-positive | no | 50 | no |
| anaerobic | no | 94.089 | no |
| aerobic | no | 96.148 | yes |
| halophile | yes | 61.127 | no |
| spore-forming | no | 98.34 | no |
| motile | no | 92.169 | no |
| glucose-ferment | yes | 72.988 | no |
| thermophile | no | 94.706 | yes |
| glucose-util | yes | 87.09 | no |
External links
@ref: 16413
culture collection no.: DSM 22601, ATCC 51748
straininfo link
- @ref: 83521
- straininfo: 43158
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Genetics | 9226909 | Spiroplasma platyhelix sp. nov., a new mollicute with unusual morphology and genome size from the dragonfly Pachydiplax longipennis. | Williamson DL, Adams JR, Whitcomb RF, Tully JG, Carle P, Konai M, Bove JM, Henegar RB | Int J Syst Bacteriol | 10.1099/00207713-47-3-763 | 1997 | Animals, Antigens, Bacterial/analysis, Cell Division, Cross Reactions, DNA, Bacterial/analysis, Genome, Bacterial, Insecta/*microbiology, Microscopy, Electron, Spiroplasma/*genetics/*immunology/ultrastructure, Sterols/metabolism | Phylogeny |
| Phylogeny | 9542070 | Revised group classification of the genus Spiroplasma. | Williamson DL, Whitcomb RF, Tully JG, Gasparich GE, Rose DL, Carle P, Bove JM, Hackett KJ, Adams JR, Henegar RB, Konai M, Chastel C, French FE | Int J Syst Bacteriol | 10.1099/00207713-48-1-1 | 1998 | Animals, Antibodies, Bacterial, Cell Membrane/ultrastructure, Classification, DNA, Bacterial/analysis, Glucose/metabolism, Insecta/*microbiology, Microscopy, Electron, Plants/*microbiology, Serologic Tests, Spiroplasma/chemistry/*classification/immunology | Metabolism |
| Genetics | 33214290 | Draft Genome Sequence of Spiroplasma platyhelix ATCC 51748, Isolated from a Dragonfly. | Green EA, Klassen JL | Microbiol Resour Announc | 10.1128/MRA.00422-20 | 2020 | Phylogeny |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 16413 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22601) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-22601 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 83521 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID43158.1 | StrainInfo: A central database for resolving microbial strain identifiers |