General

@ref: 15947

BacDive-ID: 14376

DSM-Number: 21782

keywords: 16S sequence, Bacteria, microaerophile, mesophilic

description: Spiroplasma montanense HYOS-1 is a microaerophile, mesophilic bacterium that was isolated from abdominal viscera of the tabanid fly H. opaca.

NCBI tax id

• NCBI tax id: 216943
• Matching level: species

strain history

• @ref: 15947
• history: <- J. K. Davis {2008} <- J. G. Tully <- R. F. Whitcomb, USDA, Beltsville

doi: 10.13145/bacdive14376.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/mycoplasmatota
• domain: Bacteria
• phylum: Mycoplasmatota
• class: Mollicutes
• order: Mycoplasmatales
• family: Spiroplasmataceae
• genus: Spiroplasma
• species: Spiroplasma montanense
• full scientific name: Spiroplasma montanense Whitcomb et al. 1997

@ref: 15947

domain: Bacteria

phylum: Tenericutes

class: Mollicutes

order: Entomoplasmatales

family: Spiroplasmataceae

genus: Spiroplasma

species: Spiroplasma montanense

full scientific name: Spiroplasma montanense Whitcomb et al. 1997

strain designation: HYOS-1

type strain: yes

Culture and growth conditions

culture medium

• @ref: 15947
• name: SP4-Z MEDIUM (DSMZ Medium 1076b)
• growth: yes
• link: https://mediadive.dsmz.de/medium/1076b
• composition: Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water

culture temp

• @ref: 15947
• growth: positive
• type: growth
• temperature: 30
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 15947
• oxygen tolerance: microaerophile

Isolation, sampling and environmental information

isolation

• @ref: 15947
• sample type: abdominal viscera of the tabanid fly H. opaca
• host species: Hybomitra opaca
• geographic location: Livingston, Montana
• country: USA
• origin.country: USA
• continent: North America

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_8280.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_109;96_1346;97_1598;98_6027;99_8280&stattab=map
• Last taxonomy: Spiroplasma
• 16S sequence: AY189307
• Sequence Identity:
• Total samples: 169
• soil counts: 7
• aquatic counts: 12
• animal counts: 147
• plant counts: 3

Safety information

risk assessment

• @ref: 15947
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 15947
• description: Spiroplasma montanense 16S ribosomal RNA gene, partial sequence
• accession: AY189307
• length: 1514
• database: ena
• NCBI tax ID: 216943

GC content

• @ref: 15947
• GC-content: 28±1
• method: Buoyant density centrifugation (BD)

External links

@ref: 15947

culture collection no.: DSM 21782, ATCC 51745

straininfo link

• @ref: 83519
• straininfo: 43155

literature

• topic: Phylogeny
• Pubmed-ID: 9542070
• title: Revised group classification of the genus Spiroplasma.
• authors: Williamson DL, Whitcomb RF, Tully JG, Gasparich GE, Rose DL, Carle P, Bove JM, Hackett KJ, Adams JR, Henegar RB, Konai M, Chastel C, French FE
• journal: Int J Syst Bacteriol
• DOI: 10.1099/00207713-48-1-1
• year: 1998
• mesh: Animals, Antibodies, Bacterial, Cell Membrane/ultrastructure, Classification, DNA, Bacterial/analysis, Glucose/metabolism, Insecta/*microbiology, Microscopy, Electron, Plants/*microbiology, Serologic Tests, Spiroplasma/chemistry/*classification/immunology
• topic2: Metabolism

Reference