General

@ref: 17265

BacDive-ID: 14366

DSM-Number: 23536

keywords: 16S sequence, Bacteria, microaerophile, mesophilic

description: Spiroplasma velocicrescens MQ4 is a microaerophile, mesophilic bacterium that was isolated from gut of wasp Monobia quadridens.

NCBI tax id

• NCBI tax id: 216947
• Matching level: species

strain history

• @ref: 17265
• history: <- J. K. Davis, Dept. Agronomy, Purdue Univ., West Lafayette, USA <- J. G. Tully <- T. B. Clark, USDA, Beltsville; MQ4

doi: 10.13145/bacdive14366.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/mycoplasmatota
• domain: Bacteria
• phylum: Mycoplasmatota
• class: Mollicutes
• order: Mycoplasmatales
• family: Spiroplasmataceae
• genus: Spiroplasma
• species: Spiroplasma velocicrescens
• full scientific name: Spiroplasma velocicrescens Konai et al. 1995

@ref: 17265

domain: Bacteria

phylum: Tenericutes

class: Mollicutes

order: Entomoplasmatales

family: Spiroplasmataceae

genus: Spiroplasma

species: Spiroplasma velocicrescens

full scientific name: Spiroplasma velocicrescens Konai et al. 1995

strain designation: MQ4

type strain: yes

Culture and growth conditions

culture medium

• @ref: 17265
• name: SP4-Z MEDIUM (DSMZ Medium 1076b)
• growth: yes
• link: https://mediadive.dsmz.de/medium/1076b
• composition: Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water

culture temp

• @ref: 17265
• growth: positive
• type: growth
• temperature: 37
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 17265
• oxygen tolerance: microaerophile

Isolation, sampling and environmental information

isolation

• @ref: 17265
• sample type: gut of wasp Monobia quadridens
• host species: Monobia quadridens
• geographic location: Maryland
• country: USA
• origin.country: USA
• continent: North America

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_140336.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_109;96_1346;97_1598;98_4873;99_140336&stattab=map
• Last taxonomy: Spiroplasma
• 16S sequence: AY189311
• Sequence Identity:
• Total samples: 18
• soil counts: 1
• animal counts: 16
• plant counts: 1

Safety information

risk assessment

• @ref: 17265
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 17265
• description: Spiroplasma velocicrescens 16S ribosomal RNA gene, partial sequence
• accession: AY189311
• length: 1515
• database: ena
• NCBI tax ID: 216947

External links

@ref: 17265

culture collection no.: DSM 23536, ATCC 35262

straininfo link

• @ref: 83509
• straininfo: 40955

literature

• topic: Metabolism
• Pubmed-ID: 7727271
• title: Spiroplasma velocicrescens sp. nov., from the vespid wasp Monobia quadridens.
• authors: Konai M, Whitcomb RF, Tully JG, Rose DL, Carle P, Bove JM, Henegar RB, Hackett KJ, Clark TB, Williamson DL
• journal: Int J Syst Bacteriol
• DOI: 10.1099/00207713-45-2-203
• year: 1995
• mesh: Animals, Arginine/metabolism, Base Composition, Cholesterol/metabolism, Culture Media, DNA, Bacterial, Glucose/metabolism, Serotyping, Spiroplasma/chemistry/*classification/physiology/ultrastructure, Temperature, Urea/metabolism, Wasps/*microbiology
• topic2: Phylogeny

Reference